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Showing: 3401 - 3420 of 11520 results

  9. pBRα-σ70 D581G

    Plasmid
    #53732
    Purpose
    Plpp/PlacUV5- directed synthesis of the alpha NTD fused to E. coli σ70 region 4. Used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    RNAP alpha NTD (aa 1-248) fused to E. coli sigma 70 (aa 528-613)
    Expression
    Bacterial
    Mutation
    The sigma 70 moiety carries the D581G substitution
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pSW149

    Plasmid
    #122248
    Purpose
    pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.
    Depositor
    Sarah Walker
    Article
    Liu et al Methods. 2019 Mar 26. pii: S1046-2023(18
    Insert
    TIF4631 (TIF4631 Budding Yeast)
    Tags
    Intein-Chitin-binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    122248-plasmid-map-sequence-id-245052

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Showing: 3401 - 3420 of 11520 results