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pccGFP-MalE-neg-2E11

Plasmid

#239084

Purpose

Negative control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of LRIG3

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Valine 827 to Isoleucine

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pccGFP-MalE-neg-2F12

Plasmid

#239085

Purpose

Negative control for liquid MalE complementation assay

Depositor

Alessandro Senes

Article

Anderson et al bioRxiv 2025.04.22.650048

Insert

transmembrane domain of LRIG3

Tags

MBP and ToxR

Expression

Bacterial

Mutation

Changed Valine 815 to Isoleucine

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-luxNeon

Plasmid

#229942

Purpose

Bacterial expression of a de novo designed, FRET-based neoLux1.2-mNeonGreen fusion bioluminescent probe (max. Em=518nm)

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

luxNeon

Tags

His-tag

Expression

Bacterial

Promoter

araBAD

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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JD73

Plasmid

#229482

Purpose

Adenoviral VA RNA I & II genes

Depositor

Nerea Zabaleta

Article

Doshi et al Mol Ther Methods Clin Dev. 2024 Nov 13;3

Insert

VA RNA I & II

Use

AAV and Adenoviral

Expression

Mammalian

Promoter

wt promoter

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEC2796 (pSpy0C4)

Plasmid

#220280

Purpose

integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombination

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-1-ZmUbi1-6

Plasmid

#197714

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

ZmUbi1 promoter

Expression

Plant

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pMC-1-PvUbi1-6

Plasmid

#197705

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

PvUbi1 promoter

Expression

Plant

Available Since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP13

Plasmid

#207584

Purpose

TolQR expression vector for purification (TolQ-TolR-TEV-Strep_His)

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

TolQ-TolR-TEV-Strep_His

Expression

Bacterial

Available Since

Dec. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pJS110

Plasmid

#207591

Purpose

GFP-AIA H22A expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-AIA H22A

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJMG1

Plasmid

#207594

Purpose

GFP-TonB deltaPII expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TonB deltaPII

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJV0016

Plasmid

#205133

Purpose

Expression of 2xPX-GFP in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

tandem 2xPX domain of SnxA

Use

Dictyostelium expression

Tags

EGFP

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-3-Reverse

Plasmid

#197803

Purpose

Level 1 Module 3 destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-5-Esp3I

Plasmid

#197836

Purpose

Level 1 module 5 with Esp3I expansion point

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-5-Reverse

Plasmid

#197807

Purpose

Level 1 Module 5 destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-2T-Reverse

Plasmid

#197800

Purpose

Level 1 Module 2T destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-7T-Reverse

Plasmid

#197810

Purpose

Level 1 Module 7T destination vector (reverse orientation MoClo insert)

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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BASIC_SEVA_69.11

Plasmid

#194214

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 26, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pMC-6-Integrase13-9

Plasmid

#197728

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

Integrase13 (tomato optimized)

Expression

Plant

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-L2-Donor-Esp3I

Plasmid

#197841

Purpose

Donor vector to restore L2 destination via Esp3I

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pJMC-3-Esp3I

Plasmid

#197832

Purpose

Level 1 module 3 with Esp3I expansion point

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

None

Use

Unspecified

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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