8,914 results
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Plasmid#66464PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only
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pMIH-TOMM20-Halo
Plasmid#111626PurposeFusion protein used to visualise mitochondria upon the addition of Halo specific dye, has hygromycin selectionDepositorAvailable SinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
TfR-sfGFP-myc tag-SpyCatcher003
Plasmid#133451PurposeExpresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detectionDepositorInsertTfR-sfGFP-myc tag-SpyCatcher003
TagsGSSGS and myc tagExpressionMammalianMutationContains C20 and A23 mutations that improve plasm…PromoterCMVAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
Flag-NRF1
Plasmid#34707DepositorAvailable SinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
TFEB promoter-luciferase reporter
Plasmid#66801PurposeReporter for activity of TFEB gene promoterDepositorInsertTFEB promoter (Tfeb Mouse)
UseLuciferaseAvailable SinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
CAV1-mCherry
Plasmid#27705DepositorAvailable SinceJuly 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human Erbb2-IRES-GFP
Plasmid#91888PurposeRetroviral expression of human Erbb2DepositorAvailable SinceJuly 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
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PB_tre_dCas9_VP160
Plasmid#126031PurposePiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusionDepositorInsertsdCas9 fusion with VP160
Hygromycin resistance
UseCRISPR; PiggybacExpressionMammalianMutationD10A, H840A (catalytically inactive Cas9)PromoterEF1 alpha and TREAvailable SinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22A
Plasmid#91133PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRExpressionPlantAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP_CD47_LU
Plasmid#65474PurposeGFP tagged CD47 with its own long 3'UTRDepositorInsertsSignal peptide of CD47
GFP
CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).
ExpressionMammalianAvailable SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
Pyronic/pcDNA3.1(-)
Plasmid#51308PurposePyronic, FRET sensor that allow quantitative measurement of pyruvate transport, pyruvate production and mitochondrial pyruvate consumption in intact individual cells at high temporal resolution.DepositorInsertPyronic
ExpressionMammalianPromoterCMVAvailable SinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherry-CNA35
Plasmid#61607Purposebacterial expression of N-terminal fusion protein of CNA35 with mCherryDepositorInsertmCherry and CNA35
TagsHis tagExpressionBacterialPromoterT7Available SinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV Luciferase PGK-hygro
Plasmid#18782DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLuciferase and RetroviralExpressionMammalianAvailable SinceJuly 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
mCherry-BP1-2 pLPC-Puro
Plasmid#19835DepositorInsertFragment of p53-Binding Protein 1 (TP53BP1 Human)
UseRetroviralTagsmCherryMutationFragment of human 53BP1 aa 1220 - 1711Available SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C-terminal GST
Plasmid#128025PurposeMammalian expression vector with C terminal GST tagDepositorTypeEmpty backboneTagsGSTExpressionMammalianAvailable SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO hChR2(H134R)-EYFP
Plasmid#55639PurposeFlp-Dependent ChR2-EYFPDepositorHas ServiceAAV RetrogradeInserthChR2(H134R)-EYFP
UseAAVTagsEYFPExpressionMammalianPromoterEf1aAvailable SinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.Cre.WPRE.hGH
Plasmid#105550PurposeAAV expression of Cre from GFAP promoterDepositorHas ServiceAAV PHP.eB and AAV5InsertCre
UseAAV and Cre/LoxExpressionMammalianPromoterGFAPAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG32
Plasmid#35122DepositorInserthphMX4
UseYeast replicatingAvailable SinceApril 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8f-WPRE
Plasmid#162376PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8f
UseAAVTags6xHisExpressionMammalianMutationQ315LPromoterSynapsinAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only