164,826 results

1. Ai9

   Plasmid

   #22799

   Purpose

   Rosa26 targeting vector, Cre-dependent tdtomato expression. See article for more colors.

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

   Insert

   CAG-Floxed tdTomato
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC18R6K-mini-Tn7T-Gm

   Plasmid

   #65022

   Purpose

   GmR mini-Tn7 vector, R6K replicon

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 17, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pPtGE34

   Plasmid

   #107932

   Purpose

   Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

   Depositor

   David Edgell

   Article

   Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

   Inserts

   OriT
   40SRPS8 Promoter
   ShBle
   40SRPS8 Terminator
   Cen6-ArsH4-His3
   

   Use

   CRISPR and Synthetic Biology; Episomal vector for…

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pD649-HAsp-KIR2DL4-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156662

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   KIR2DL4 (KIR2DL4 Human)
   

   Use

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG424-10X-tRNA

   Plasmid

   #70125

   Purpose

   Yeast high-copy TRP1 plasmid expression 10 intronless S. cerevisiae introns

   Depositor

   Jay Hesselberth

   Article

   Cherry et al RNA. 2018 Mar;24(3):313-323. doi: 10.126

   Insert

   10X-tRNA block
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   None – SUP4 pol III promoter provided by insert

   Available Since

   April 21, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pAAV-Ef1a-mCherry

   Plasmid

   #114470

   Purpose

   mCherry Control

   Depositor

   Karl Deisseroth

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNCS mStrawberry

   Plasmid

   #91768

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)

   Depositor

   Roger Tsien, Erik Rodriguez

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Insert

   monomeric Strawberry
   

   Use

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCXB-EBNA1

   Plasmid

   #41857

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   EBNA-1 (EBNA-1 human herpesvirus 4)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-SAM-Dest

   Plasmid

   #102563

   Purpose

   Piggybac transposon DEST vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex. It is used for insertion of multiple U6-sgRNA expression cassettes.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   N/A

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CMV RasV12 Neo (w108-1)

    Plasmid

    #22259

    Depositor

    Eric Campeau

    Insert

    HRAS G12V (HRAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    G12V mutant

    Promoter

    Available Since

    Jan. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. PNCA-GFP

    Plasmid

    #67789

    Purpose

    Use together with pCMV-intron and VSV-G to package MMLV reporter virus

    Depositor

    Stephen Goff

    Article

    Ooi et al Epigenetics Chromatin. 2010 Sep 24;3(1):

    Insert

    MMLV-LTR-GFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pPtPBR11

    Plasmid

    #80386

    Purpose

    Episome vector for Phaeodactylum tricornutum (pBR322-based)

    Depositor

    Philip Weyman

    Type

    Empty backbone

    Use

    Synthetic Biology; : episomal vector for phaeodac…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. CA-Stat3-t2A-mCherry

    Plasmid

    #127540

    Purpose

    Expresses constitutively active (CA) mouse Stat3 and mCherry via t2A linker.

    Depositor

    Jennifer Mitchell

    Article

    Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A662C and N664C. Residues changed to Cys renders …

    Promoter

    CMV

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. GPR87-DuET

    Plasmid

    #213293

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR87 (GPR87 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221_SLC6A8

    Plasmid

    #131915

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC6A8 (SLC6A8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 15, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. Nuclear Myosin I YFP

    Plasmid

    #60241

    Purpose

    Expresses nuclear isoform of myosin IC attached to YFP and an NLS

    Depositor

    Primal de Lanerolle

    Article

    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

    Insert

    Nuclear Myosin 1 (Myo1c Mouse)
    

    Use

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX307-CRBN-3F-BirA

    Plasmid

    #220146

    Purpose

    Expresses CRBN-BirA for CRBN substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    Cereblon (CRBN Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pICH47742::p35S-PE2max-NC::tEURb7

    Plasmid

    #227693

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available Since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. hLRP1-pEZT-BM

    Plasmid

    #177985

    Purpose

    Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells

    Depositor

    Lora Hooper

    Article

    Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

    Insert

    LDL receptor related protein 1 (LRP1 Human)
    

    Use

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK5-EGFP-Tau

    Plasmid

    #46904

    Purpose

    expresses EGFP tagged Tau in mammalian cells

    Depositor

    Karen Ashe

    Article

    Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    This WT htau construct contains human four-repeat…

    Promoter

    CMV

    Available Since

    July 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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