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xCIAR_pcDNA5/FRT/TO
Plasmid#86504PurposeExpresses inactive control CIAR (xCIAR) in mammalian cells. Used to generate Flp-In stables.DepositorInsertxCIAR (SOS1 Human)
UseFrtTagsExpressionMammalianMutationF929A and T968L in SOScatPromoterCMV (tet operator)Available sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-FLAG-SLC38A9.1 Y71A
Plasmid#71865Purposelentiviral stable expressionDepositorInsertSLC38A9.1 (SLC38A9 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationcodon optimized, Tyrosine 71 mutated to AlaninePromoterCMVAvailable sinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLPC-NMYC-mTRF1deltaBLM
Plasmid#64163PurposeRetroviral vector expressing mouse TRF1 lacking BLM-binding motifs with N-terminal Myc tagDepositorInsertmTRF1 (Terf1 Mouse)
UseRetroviralTagsMycExpressionMammalianMutationDeleted amino acids 313-316 and 339-342 (both BLM…PromoterCMVAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
YF-AIP
Plasmid#61525PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with EYFP and AIPDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP and EYFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
CF-AIP
Plasmid#61526PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with ECFP and AIPDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP and ECFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQCXIB-FLAG-hDIXDC1-L-S592A
Plasmid#61225PurposeRetrovirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseRetroviralTagsFLAGExpressionMutationChanged Serine 592 to AlaninePromoterAvailable sinceMarch 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-TER+-shDIXDC1-Ms-58
Plasmid#61236PurposeEntry clone encoding shRNA to mouse DIXDC1DepositorInsertDixdc1 (Dixdc1 Mouse)
UseEntr cloneTagsExpressionMutationPromoterH1/TOAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-TER+-shDIXDC1-Ms-57
Plasmid#61235PurposeEntry clone encoding shRNA to mouse DIXDC1DepositorInsertDixdc1 (Dixdc1 Mouse)
UseEntr cloneTagsExpressionMutationPromoterH1/TOAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Hygro-FLAG-hDIXDC1 S592A
Plasmid#61214PurposeRetrovirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseRetroviralTagsFLAGExpressionMutationChanged Serine 592 to AlaninePromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLentiX2-PURO-shDIXDC1-Ms-58
Plasmid#61233PurposeLentivirus expressing shRNA to mouse DIXDC1DepositorInsertDixdc1 (Dixdc1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Blast-FLAG-hDIXDC1 S592A
Plasmid#61221PurposeLentivirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseLentiviralTagsFLAGExpressionMutationChanged Serine 592 to AlaninePromoterPGKAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV/TO-Puro-FLAG-hDIXDC1 S592A
Plasmid#61219PurposeLentivirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseLentiviralTagsFLAGExpressionMutationChanged Serine 592 to AlaninePromoterCMV/TOAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWZLblasti-HA-MEK5 CBM-E
Plasmid#53169PurposeExpresses HA tagged human MEK5 L280AH281AM323AQ332A from blasticidin resistance retroviral vectorDepositorInsertMEK5 (MAP2K5 Human)
UseRetroviralTagsHAExpressionMammalianMutationL280A/H281A/M323A/Q332APromoterAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
SLP2a-TIR-GFP
Plasmid#52738PurposeRetroviral vector expresses the lipid binding domain of SLP2a fused to the TIR of hTIRAP and GFP. Also contains IRES-hCD2 as selectable markerDepositorInsertToll/IL-1 Receptor adaptor protein (TIRAP Human)
UseRetroviralTagsC2A domain of Murine SLP2a and GFPExpressionMammalianMutationDeleted amino acids 1-85PromoterAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
TRS6E1b-luc(-732/-721) mutant 4
Plasmid#45387DepositorInsert6 copies of hPAI-1 promoter -732/-721 four point mutation (SERPINE1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationmutated from agacaaggttgt to acactaggatgaPromoterAvailable sinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-PH-ARNO2G-I303Ex2
Plasmid#116868PurposePIP3 biosensorDepositorInsertCYTH2 (CYTH2 Human)
UseTagsEGFP and X.leavis map2k1.L(32-44)ExpressionMammalianMutationamind acids 252-399, isoleucine 303 changed to gl…PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
ITSN-mcherry-sspB
Plasmid#85220PurposeRole of Cdc-42 selective GEF domain from ITSN in immune cell migrationDepositorInsertsUseTagsNAExpressionMammalianMutationR73QPromoterCMVAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA
Plasmid#154867PurposeFlp-dependent hM4D(Gi) DREADD-mCherryDepositorHas ServiceAAV Retrograde and AAV8InserthM4D(Gi)-mCherry
UseAAV; Flp-frtTagsmCherryExpressionMammalianMutationPromoterHuman Synapsin-1Available sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
EF.STAT3C.Ubc.GFP
Plasmid#24983Purpose3rd generation lentiviral expression of constitutively active Stat3DepositorInsertSTAT3 (STAT3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationA662C, N664C (constitutively active)PromoterAvailable sinceMay 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pGolt-mCherry / pGolt3-mCherry
Plasmid#73297PurposeVisualization of Dendritic Golgi SatelliteDepositorInsertsER export signal of Scap
ER export signal of Scap
TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
UseTagsER export and mCherryExpressionMammalianMutationPromoterCMV immediate early promoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FLAG-GFP-OSBP
Plasmid#134659PurposeFlag and GFP-tagged OSBPDepositorInsertOSBP (OSBP Human)
UseLentiviralTagsFLAG and GFPExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ALK6 wt
Plasmid#80882Purposemammalian expression of ALK6 WTDepositorInsertALK6 (Bmpr1b Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-BTC-ScNeo
Plasmid#209898PurposeTo monitor the status of Betacellulin, the plasmid encodes a recombinant BTC fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertBTC-ScNeo (BTC Human)
UseLentiviralTagsmNeonGreen and mScarletExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-NRG1-ScNeo
Plasmid#209900PurposeTo monitor the status of NRG1, the plasmid encodes a recombinant NRG1 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertNRG1-ScNeo (NRG1 Human)
UseLentiviralTagsmNeonGreen and mScarletExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL403
Plasmid#119953PurposeExpresses GPPS and LimS, for the production of GPP and (S)-limonene, in Escherichia coli.DepositorInsertsgpps
limS
UseTagsExpressionBacterialMutationN-terminal truncation of 56 amino acid residues (…PromoterAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLPC-MYC-hTRF1
Plasmid#64164PurposeRetroviral vector expressing human TRF1 with N-terminal MYC tagDepositorInserthTRF1 (TERF1 Human)
UseRetroviralTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
CIBN-GFP-Sec61B
Plasmid#104177PurposeExpresses fusion of CIB1 (1-170) with GFP and Sec61BDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFPC2-Gephyrin P1
Plasmid#68815Purposeexpression of fluorescent tagged Geph in mammalian cellsDepositorInsertGephyrin (Gphn Rat)
UseTagsEGFPExpressionMammalianMutationsilent mutations on internal EcoRI site. Gephyri…PromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR3-FLAG-Gephyrin P1
Plasmid#68816PurposeFlag tagged Geph mammalian expressionDepositorInsertGephyrin (Gphn Rat)
UseTags3xFLAGExpressionMammalianMutationsilent mutations on internal EcoRI site. Gephyrin…PromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only
