We narrowed to 15,050 results for: pET

1. MZB131

   Plasmid

   #217839

   Purpose

   Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged AP3D1 adaptin appendage (920-1153) for pulldown assays

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   AP3D1 (AP3D1 Human)
   

   Tags

   10x His, HaloTag, and SUMO (SMT3)

   Expression

   Bacterial

   Mutation

   Deleted residues 1-919

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MZB150

   Plasmid

   #217840

   Purpose

   Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (52-252) of Cytohesin-2 (CYTH2/ARNO) for rapid nucleotide exchange and activation of Arf GTPases

   Depositor

   Richard Baker

   Article

   Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   ARNO (CYTH2 Human)
   

   Tags

   10x His and SUMO (SMT3)

   Expression

   Bacterial

   Mutation

   Deleted residues 1-51 and 253-400

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. small ubiquitin-like modifier 1 (SUMO1)

   Plasmid

   #221052

   Purpose

   Expression of small ubiquitin-like modifier 1 (SUMO1) protein under T7 promoter

   Depositor

   Michael Rosen

   Article

   Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

   Insert

   SUMO 1 (SUMO1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACB108

   Plasmid

   #222207

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACB109

   Plasmid

   #222208

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA (K10N)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed Lys10 to Asn

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACB110

   Plasmid

   #222209

   Purpose

   For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tag

   Depositor

   Gregg Beckham

   Article

   Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

   Insert

   fghA (G258D)
   

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   Changed Gly258 to Aspartate

   Promoter

   T7

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. GB1-ΔN-CHMP4C(G154C)

   Plasmid

   #190783

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, G154C)

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-CHMP4C(121-233, G154C) (CHMP4C Human)
   

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   G154C

   Available Since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. GB1-ΔN-CHMP4C(M165C)

   Plasmid

   #190784

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, M165C)

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-CHMP4C(121-233, M165C) (CHMP4C Human)
   

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   M165C

   Available Since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GB1-ΔN-CHMP4C(S191C)

   Plasmid

   #180027

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-CHMP4C(121-233, S191C).

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

   Insert

   GB1-His6-TEV-CHMP4C(121-233, S191C) (CHMP4C Human)
   

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   S191C

   Available Since

   June 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBtPIPLC_N168C His

    Plasmid

    #173809

    Purpose

    Expression and purification of the mature form of Bacillus thuringiensis phosphaatidyl inositol specific phospholipase C (PI-PLC), UniProt ID P08954 from E. coli BL21 CodonPlus (DE3) RIL cells

    Depositor

    Anne Gershenson, Mary F Roberts

    Article

    He et al J Biol Chem. 2015 Jul 31;290(31):19334-4

    Insert

    Bacillus thuringiensis 1-phosphatidylinositol phosphodiesterase
    

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Asn168Cys for labeling

    Promoter

    T7

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3R112C/T180CHFm

    Plasmid

    #85091

    Purpose

    Expresses PCNA3 variant (R112C/T180C) fused to heme-FMN domain of P450 BM3 variant (A74G) in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    The R112C/T180C variant of PCNA3 fused to Heme-FMN domain of P450 BM3
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C in PCNA3, A74G in heme-FMN domain of …

    Promoter

    T7 promoter

    Available Since

    Dec. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. HASP_HUMAN_D0

    Plasmid

    #79730

    Purpose

    This plasmid encodes the kinase domain of HASP. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    HASP
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. KCC2D_HUMAN_D0

    Plasmid

    #79734

    Purpose

    This plasmid encodes the kinase domain of KCC2D. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KCC2D
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

14. AAPK2_HUMAN_D0

    Plasmid

    #79738

    Purpose

    This plasmid encodes the kinase domain of AAPK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    AAPK2
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. KCC4_HUMAN_D0

    Plasmid

    #79744

    Purpose

    This plasmid encodes the kinase domain of KCC4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KCC4 (SLC12A7 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. KCC1G_HUMAN_D0

    Plasmid

    #79745

    Purpose

    This plasmid encodes the kinase domain of KCC1G. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KCC1G
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. KCC2A_HUMAN_D0

    Plasmid

    #79746

    Purpose

    This plasmid encodes the kinase domain of KCC2A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KCC2A
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. KC1G2_HUMAN_D0

    Plasmid

    #79716

    Purpose

    This plasmid encodes the kinase domain of KC1G2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    KC1G2
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

19. STK10_HUMAN_D0

    Plasmid

    #79722

    Purpose

    This plasmid encodes the kinase domain of STK10. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    STK10 (STK10 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. MK03_HUMAN_D0

    Plasmid

    #79688

    Purpose

    This plasmid encodes the kinase domain of MK03. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MK03
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

21. PAK4_HUMAN_D0

    Plasmid

    #79698

    Purpose

    This plasmid encodes the kinase domain of PAK4. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    PAK4 (PAK4 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

22. PAK6_HUMAN_D0

    Plasmid

    #79703

    Purpose

    This plasmid encodes the kinase domain of PAK6. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    Nicholas Levinson, John Chodera, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    PAK6 (PAK6 Human)
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

23. RP1B

    Plasmid

    #26563

    Depositor

    Wolfgang Peti

    Article

    Peti et al Protein Expr Purif. 2007 Jan . 51(1):1-1

    Type

    Empty backbone

    Tags

    His6, TEV cleavage site, and Thio-6

    Expression

    Bacterial

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

24. pCIneo-hCHD7

    Plasmid

    #89458

    Purpose

    constitutive expression of human CHD7 in mammalian cells

    Depositor

    Peter Scacheri

    Insert

    CHD7 (CHD7 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. pMTP170 (pBAD33_CRISPR(Tn6900)-entry)

    Plasmid

    #164263

    Purpose

    Entry vector for cloning user-defined spacers in the Tn6900 CRISPR array.

    Depositor

    Joseph Peters

    Article

    Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    Arabinose

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCIneo-hCHD7-Kozak ATG 3' 3x Flag-bGH polyA

    Plasmid

    #89459

    Purpose

    constitutive expression of human CHD7 in mammalian cells

    Depositor

    Peter Scacheri

    Insert

    CHD7 (CHD7 Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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27. pCIneo-hCHD7-Kozak ATG 3' HA-bGH polyA

    Plasmid

    #89460

    Purpose

    constitutive expression of human CHD7 in mammalian cells

    Depositor

    Peter Scacheri

    Insert

    CHD7 (CHD7 Human)
    

    Tags

    hinge + HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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28. pMTP112 (pMS26_miniTn6900(KanR))

    Plasmid

    #164260

    Purpose

    Delivery vector to insert the miniTn6900(KanR) element into the E. coli chromosome at the neutral Tn7 attachment site inside a miniTn7 element.

    Depositor

    Joseph Peters

    Article

    Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

    Insert

    miniTn6900 element
    

    Use

    Bacterial delivery vector for tn7 att site

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. pJMP1039

    Plasmid

    #119239

    Purpose

    expresses Tn7 transposase

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    tnsABCD
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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30. pJMP1054

    Plasmid

    #119241

    Purpose

    construction intermediate

    Depositor

    Oren Rosenberg, Jason Peters, Carol Gross

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Dec. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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