We narrowed to 9,370 results for: bli

1. 4IKP

   Plasmid

   #60080

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CARM1:JMC049-A06:C226836 (CARM1 Human)
   

   Use

   Baculovirus expression

   Tags

   6xHis

   Mutation

   See comments

   Promoter

   polyhedrin

   Available Since

   Oct. 22, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pCAGGS-N2c(G)

   Plasmid

   #73481

   Purpose

   N2c Glycoprotein expression vector

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   N2c(G)
   

   Expression

   Mammalian

   Available Since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pAAV-hSyn-GluA1-scFv-mNeon-KDEL

   Plasmid

   #230056

   Purpose

   AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA1 single chain variable fragment (scFv)
   

   Use

   AAV

   Tags

   ER retrieval motif (KDEL) and mNeon

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. DDX3X in pENTR

   Plasmid

   #216572

   Purpose

   gateway cloning donor vector containing DDX3X

   Depositor

   Christopher Loewen, Douglas Allan

   Insert

   DDX3X (DDX3X Human)
   

   Use

   Gateway entry plasmid

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-DIO-HA-rM3D(Gs)-IRES-mCitrine

   Plasmid

   #50456

   Purpose

   Double floxed Gs-coupled rM3D-IRES-mCitrine under the control of human synapsin promoter

   Depositor

   Bryan Roth

   Insert

   rM3D-IRES-mCitrine (CHRM3 Human)
   

   Use

   AAV

   Tags

   HA

   Mutation

   See supplemental documents for DREADD mutations

   Promoter

   human Synapsin 1

   Available Since

   April 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB660-CYCD3;1

   Plasmid

   #140751

   Purpose

   Inducing plant cells to enter mitosis

   Depositor

   Bo Liu

   Article

   Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

   Insert

   Cyclin D3;1 (CYCD3;1 Mustard Weed)
   

   Tags

   TagRFP

   Expression

   Plant

   Promoter

   CaMV 35 promoter

   Available Since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

   Plasmid

   #66708

   Purpose

   expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

   Depositor

   Charles Gersbach

   Insert

   S.Pyogenes VP64-dCas9-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   VP64

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-4T1-14-3-3 gamma GST

   Plasmid

   #13280

   Depositor

   Michael Yaffe

   Article

   Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

   Insert

   14-3-3 gamma (Ywhag Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

9. Hs.KRAS4b G12S

   Plasmid

   #83144

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12S mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   G12S

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG426-GAL-Alpha-synuclein-E46K

    Plasmid

    #181723

    Purpose

    Galactose inducible expression of Alpha-synuclein-E46K

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Expression

    Yeast

    Mutation

    E46K

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. JMC044-B12

    Plasmid

    #58752

    Purpose

    bacterial expression of PDB 4NW3

    Depositor

    Cheryl Arrowsmith

    Insert

    PDB:4NW3 (KMT2A Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Bacterial expression for structure determination;…

    Promoter

    T7

    Available Since

    Aug. 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. Hs.NRAS G12V

    Plasmid

    #83174

    Purpose

    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), G12V mutation

    Depositor

    Dominic Esposito

    Insert

    neuroblastoma RAS viral oncogene homolog (NRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12V

    Available Since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDF1-mAtf4-HT-puro

    Plasmid

    #101795

    Purpose

    Lentiviral vector containing C-terminally Halo-tag tagged mouse ATF4

    Depositor

    Seiichi Oyadomari

    Insert

    Activating transcription factor 4 (Atf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Halo tag

    Expression

    Mammalian

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. ALDH18A1

    Plasmid

    #38947

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ALDH18A1 (ALDH18A1 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. MS2_SC2_S_Delta

    Plasmid

    #175950

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    Delta (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pAAV-hSyn-GluA1-scFv-Halo

    Plasmid

    #230055

    Purpose

    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Use

    AAV

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. PTPN11 in pENTR

    Plasmid

    #216604

    Purpose

    gateway cloning donor vector containing PTPN11

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    Gateway entry plasmid

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-TREtight-mTagBFP2-N2cG

    Plasmid

    #192838

    Purpose

    plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

    Depositor

    Ian Wickersham

    Insert

    mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TREtight

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PE-SUMO CRYAB

    Plasmid

    #217670

    Purpose

    For expression of SUMO tagged human alpha B crystallin

    Depositor

    Larry David

    Insert

    CRYAB (CRYAB Human)
    

    Tags

    6XHis and Smt3

    Expression

    Bacterial

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRE-T2-IRES-His6-Ubiquitin

    Plasmid

    #107107

    Purpose

    Designed to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.

    Depositor

    Michael E. Wright

    Insert

    human ubiquitin UBB (UBB Human)
    

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    Tet

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. psd44-G13QL

    Plasmid

    #46828

    Purpose

    expression of mutant G protein alpha 13 in mammalian cells

    Depositor

    Agnese Mariotti

    Insert

    GNA13 (GNA13 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Q226L (The mutation reduces GTPase activity resul…

    Promoter

    PGK promoter

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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22. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid

    #201151

    Purpose

    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

    Depositor

    Moshe Szyf

    Inserts

    SadCas9-KRAB
    gRNA-TRE
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Mutation

    gRNA sequence: ATCAGTGATAGAGAACGTATG

    Available Since

    July 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBAD_IPF2.0

    Plasmid

    #163124

    Purpose

    A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

    Depositor

    Julie Biteen

    Article

    Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

    Insert

    IFP2.0
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CAMK2G

    Plasmid

    #38894

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CAMK2G (CAMK2G Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pRSET-His-APEX2

    Plasmid

    #201471

    Purpose

    Recombinant soluble APEX2 for SLAPSHOT labeling

    Depositor

    Yuh-Nung Jan

    Article

    Tuomivaara et al Commun Biol. 2024 Aug 29;7(1):1060. doi:

    Insert

    APEX2
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV2-CAGIG-LAMP1-mGL

    Plasmid

    #222394

    Purpose

    To express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.

    Depositor

    Pantelis Tsoulfas

    Insert

    LAMP1 and mGreenlantern (LAMP1 Homo Sapiens, Human, Synthetic)
    

    Use

    AAV

    Tags

    LAMP1 and mGreenLantern

    Expression

    Mammalian

    Promoter

    CAG or CAGIG

    Available Since

    Sept. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

    Plasmid

    #159912

    Purpose

    Mutagenesis of Kcnd3

    Depositor

    Larry Zweifel

    Insert

    Kcnd3 (Kcnd3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

    Plasmid

    #105715

    Purpose

    In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.

    Depositor

    Rylan Larsen

    Has Service

    AAV5

    Insert

    synaptophysin-GCaMP6s
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    Ef1a, Human elongation factor-1 alpha

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAG426-GAL-Alpha-synuclein-H50Q

    Plasmid

    #181724

    Purpose

    Galactose inducible expression of Alpha-synuclein-H50Q

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Expression

    Yeast

    Mutation

    H50Q

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pScDD2_ERdd

    Plasmid

    #109047

    Purpose

    destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).

    Depositor

    Thomas Wandless

    Insert

    ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
    

    Tags

    yeast enhanced GFP with 8 amino acids TSGRGEGQ li…

    Expression

    Yeast

    Mutation

    3 mutations : E380G, R434G, N532S

    Promoter

    SOD1

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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31. CD44Z-c011

    Plasmid

    #175455

    Purpose

    Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Tags

    His6

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLKO.1-blast shKGA

    Plasmid

    #110420

    Purpose

    shKGA, silence glutaminase KGA isoform, blasticidin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 (RNA Pol III)

    Available Since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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33. pTP-AAV-ChATen-mCherry

    Plasmid

    #193741

    Purpose

    This AAV vector contains a ChAT enhancer that drives mCherry expression in spinal cord skeletal motor neurons. Specificity of vector not characterized outside the mouse spinal cord.

    Depositor

    Hynek Wichterle

    Insert

    ChAT enhancer, mini promoter, synthetic intron, mCherry
    

    Use

    AAV and Mouse Targeting

    Expression

    Mammalian

    Promoter

    1000 bp ChAT enhancer with a 32 bp minimal promot…

    Available Since

    Oct. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pEC2735 (pSpy0C2)

    Plasmid

    #220279

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p-dCas9-p300-Hygro

    Plasmid

    #104411

    Purpose

    transient expression of dCas9-p300 fusion protein

    Depositor

    Stephan Beck

    Insert

    p300 (EP300 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. saposin B^W

    Plasmid

    #161759

    Purpose

    Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

    Depositor

    Sam Gellman

    Article

    Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

    Insert

    saposin B R38_W
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    changed Arginine 38 to Tryptophan

    Promoter

    T7

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. DOT1L

    Plasmid

    #36196

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DOT1L (GeneScript Synthesized) (DOT1L Human)
    

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acid residues 1-420

    Available Since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

38. Hs.KRAS4b G12R

    Plasmid

    #83143

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12R mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12R

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. DOT1L

    Plasmid

    #40736

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    DOT1L (DOT1L Human)
    

    Tags

    6xHis TEV

    Expression

    Bacterial

    Available Since

    Oct. 18, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

40. pBabe-Kras G12V-T35S

    Plasmid

    #58900

    Purpose

    Expresses human Kras with G12V and T35S mutations

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    G12V and T35S mutations (effector binding mutant)

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only