164,577 results

1. pCMV enAsCas12f-HKRA-sgRNA_v7

   Plasmid

   #208357

   Purpose

   Expression of enAsCas12f-HKRA and sgRNA_v7 in mammalian cells

   Depositor

   Atsushi Hoshino

   Article

   Hino et al Cell. 2023 Sep 22:S0092-8674(23)00963-7.

   Insert

   enAsCas12f-HKRA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   I123H/D195K/D208R/V232A

   Promoter

   CMV

   Available Since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGP-AAV-syn-iGABASnFR2-WPRE

   Plasmid

   #218874

   Purpose

   AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Has Service

   AAV1 and AAV5

   Insert

   iGABASnFR2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   S99A F102Y F104Y L178S

   Promoter

   Synapsin

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. hLRP1-pEZT-BM

   Plasmid

   #177985

   Purpose

   Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells

   Depositor

   Lora Hooper

   Article

   Bang et al Science. 2021 Sep 17;373(6561):eabf9232.

   Insert

   LDL receptor related protein 1 (LRP1 Human)
   

   Use

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21a-Streptavidin-Alive

   Plasmid

   #20860

   Purpose

   Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

   Depositor

   Alice Ting

   Article

   Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

   Insert

   Streptavidin-Alive
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Wt gene

   Promoter

   Available Since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pK18sB

   Plasmid

   #177838

   Purpose

   Plasmid for gene replacement using kanamycin/sucrose selection/counterselection. Derived from pK18mobsacB but reduced in size and lacking oriT required for conjugation.

   Depositor

   Christopher Johnson, Gregg Beckham

   Article

   Jayakody et al Energy Environ. Sci., 2018,11, 1625-1638

   Type

   Empty backbone

   Use

   Bacterial gene replacement

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-VPR-c- Myc_NLS-mCherry-WPRE

   Plasmid

   #176269

   Purpose

   Expression of dCasMINI-V4-VPR-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   dCasMINI-V4-VPR-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Oct. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pATetO 6XHis-Phusion

   Plasmid

   #206210

   Purpose

   Inducible expression of DNA polymerase

   Depositor

   Andrew Ellington

   Article

   Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

   Insert

   Phusion DNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+) NAPstar3b

   Plasmid

   #232736

   Purpose

   Expresses NADPH/NADP+ biosensor NAPstar3b in mammalian cells.

   Depositor

   Bruce Morgan

   Article

   Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

   Insert

   NAPstar3b
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAttBF3Hyg

   Plasmid

   #244885

   Purpose

   Hyg resistant attB phiC31 recombination-mediation insertion (RMI) vector for C. elegans

   Depositor

   Michael Nonet

   Article

   Beck et al MicroPubl Biol. 2025 Sep 2;2025:10.17912

   Type

   Empty backbone

   Use

   Phic31 recombinase mediated insertion vector

   Tags

   Expression

   Worm

   Mutation

   Promoter

   none

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid

    #220800

    Purpose

    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

    Depositor

    Elizabeth Kirby

    Article

    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

    Insert

    Mars1 (Mars1 Mouse)
    

    Use

    Lentiviral

    Tags

    2xFLAG and T2A-mCherry

    Expression

    Mutation

    mutation in MetRS to change aa 274 from L to G

    Promoter

    EF1a

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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