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Plasmid#232137Purposezebrafish dgat1a anti-sense in situ probe, linearize with NotI, SP6 polymeraseDepositorInsertdgat1a (dgat1a Zebrafish)
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO-mogat2 in situ probe
Plasmid#232139Purposezebrafish mogat2 anti-sense in situ probe, linearize with NotI, SP6 polymeraseDepositorInsertmogat2 (mogat2 Zebrafish)
UseIn vitro transcriptionTagsExpressionMutationPromoterSP6/T7Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB215
Plasmid#221767PurposeBacterial expression of human Arf6 (Q67L) with C-terminal 'self-cleaving' CPD-10xHis tagDepositorInsertArf6 (ARF6 Human)
UseTagsCPD-10xHisExpressionBacterialMutationQ67LPromoterT7 PromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB218
Plasmid#221769PurposeBacterial expression of human Arf4 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tagDepositorInsertArf4 (ARF4 Human)
UseTagsCPD-10xHisExpressionBacterialMutationQ71LPromoterT7 PromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB219
Plasmid#221770PurposeBacterial expression of human Arf5 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tagDepositorInsertArf5 (ARF5 Human)
UseTagsCPD-10xHisExpressionBacterialMutationQ71LPromoterT7 PromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB220
Plasmid#221771PurposeBacterial expression of human Arl1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tagDepositorInsertArl1 (ARL1 Human)
UseTagsCPDExpressionBacterialMutationQ71LPromoterT7 PromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1:ChrA-C63S-C66S
Plasmid#225082PurposeExpresses precursor peptide ChrA-C63S/C66S in E.coliDepositorInsertChrA-C63S/C66S
UseTagsHis6ExpressionBacterialMutationC63S/C66SPromoterT7Available sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVF18
Plasmid#229456PurposeExpresses IraD (aa17-130) with His-tagDepositorInsertIraD (aa 17-130 only)
UseTags6xHisExpressionBacterialMutationaa 17-130 onlyPromoterT7Available sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG
Plasmid#200792PurposeMammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAGDepositorInsertKIF5A (Kif5a Mouse)
UseTagsCLIPf and SNAPf, HisTag, FLAGExpressionMammalianMutationΔ954-1027PromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG
Plasmid#200798PurposeMammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAGDepositorInsertKIF5A (Kif5a Mouse)
UseTagsCLIPf and SNAPf, HisTag, FLAGExpressionMammalianMutation411-680PromoterCMV & T7Available sinceDec. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM59
Plasmid#227655PurposepETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Δ78–81)DepositorUseTagsH6-PreScission and PreScission-StrepExpressionBacterialMutation1-179 and amino acids 2-239, Δ78–81PromoterT7Available sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarL YdfI Flag
Plasmid#225650PurposeExpresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.DepositorInsertNarL-YdfI (narL Synthetic)
UseCell free expressionTagsFLAGExpressionBacterialMutationNarL with native DNA-binding domain replaced with…PromoterT7Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-Cas9-FCPF
Plasmid#220132PurposeExpresses Cas9-FCPF in E.coliDepositorInsertCas9-FCPF (cas9 S. pyogenes)
UseTagsHis and NLSExpressionBacterialMutationPromoterT7Available sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
iNicSnFR12
Plasmid#227142PurposeGenetically encoded fluorescent biosensor for nicotine detectionDepositorInsertiNicSnFR12
UseTags6xHis-HA and MycExpressionBacterialMutationPromoterT7Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
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WDV
Plasmid#226983PurposeExpresses the HUH endonuclease WDV, used as a no ligand control for DNA tension sensor experimentsDepositorInsertWDV (repA )
UseTags6xHis and SUMOExpressionBacterialMutationPromoterT7Available sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB65
Plasmid#226319PurposeE. coli protein expression for protein purificationDepositorInsert6xHis- (VTG to AAA MR1-6)-strep
UseTags6xHis and StrepExpressionBacterialMutationN-terminal domain deleted, C-terminal domain dele…PromoterT7Available sinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-1:ChrH-ChrI
Plasmid#225083PurposeExpresses multinuclear non-heme iron dependent oxidative enzyme ChrH and partner protein ChrI in E.coliDepositorInsertChrH
UseTagsExpressionBacterialMutationWTPromoterT7Available sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-yhaM-RFP
Plasmid#207651PurposeOverexpression of native L-cysteine desulfidase and RFP in E. coli MG1655DepositorInsertyhaM
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2
Plasmid#221082PurposePlasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.DepositorInsertACC-msfGFP[r5M]_ACC-mEBFP2
UseSynthetic BiologyTagsExpressionMutationPromoterCMV, T7Available sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-Cm-CspB
Plasmid#215400PurposeCm NCPPB382 cspB codon-optimized for E. coli recombinant protein expression (6xHis-CspB)DepositorInsertcspB
UseTags6xHis TagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-Cm-CspA2
Plasmid#215399PurposeCm NCPPB382 cspA2 codon-optimized for E. coli recombinant protein expression (6xHis-CspA2)DepositorInsertcspA2
UseTags6xHis TagExpressionBacterialMutationPromoterT7 promoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Rac1/13C-FKBP-MBP
Plasmid#214295PurposeExpress split-Rac1 fragment fused with FKBP and MBPDepositorInsertSplit-Rac1 fragment fused with FKBP (RAC1 Human)
UseTagsMaltose-binding protein (MBP)ExpressionBacterialMutationRac1-Q61LPromoterT7Available sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-Gal4-p53
Plasmid#171076PurposeExpresses Gal4-p53 fusion protein in bacteriaDepositorInsertGal4 DBD (NEWENTRY Budding Yeast)
UseTagsHis and p53(1-85)ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FKBP-empty
Plasmid#221332PurposeProtein overexpression in bacteriaDepositorInsert6xHis-3xFKBP empty scaffold
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-RSET-iGABASnFR2(no bind)-6xHis
Plasmid#218887PurposeBacterial expression of improved GABA sensor control (no change in fluorescence)DepositorInsertiGABASnFR2(no bind)
UseTags6xHisExpressionBacterialMutationS99A F102G R168PPromoterT7Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet_OPTN-thrombin-GST
Plasmid#216843PurposeFor expression in E. coli of OPTN (Internal ref. SMC1789)DepositorInsertOptineurin (OPTN Human)
UseTagsGSTExpressionBacterialMutationPromoterT7 promoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
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PDE4B-Sensor
Plasmid#208929Purposein vitro JNK-Sensor for luminescent detection of JNK-activityDepositorInsertPDE4B docking sequence+Phospho site and Smbit (PDE4B Human)
UseTagsHISx6, MBP, and SmbitExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP-pTac-himar-apraR
Plasmid#199995PurposeLanding Pad with T7 RNAP circuit variant T15. pIP1443 seed promoter. pTac-himar transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. For more information look below.DepositorInsertLanding Pads
UseTagsExpressionBacterialMutationhimar frameshiftPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-Topo-kcnk5a
Plasmid#208988PurposeRiboprobe generationDepositorInsertkcnk5a (kcnk5a Zebrafish)
UseCloningTagsExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-Topo-kcnk9
Plasmid#208992PurposeRiboprobe generationDepositorInsertkcnk9 (kcnk9 Zebrafish)
UseCloningTagsExpressionMutationWithout STOP codonPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-15b-aδNb072
Plasmid#204627PurposeExpresses the anti-IgD nanobody 072 (aδNb072) in the bacterial periplasm. aδNb072 is an orthosteric inhibitor of human anti-Phl p 7 antibodies of clone 102.1F10.DepositorInsertaδNb072
UseTagsHis6 and TEV protease cleavage siteExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only