We narrowed to 10,458 results for: T7

1. pCellFree_G03 SMAD2

   Plasmid

   #67095

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   SMAD2 (SMAD2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pLz74_Rubisco_CsoS2_Npeptide_fusion

   Plasmid

   #140840

   Purpose

   Same as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallization

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   Form IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS
   

   Use

   Tags

   CsoS2 N-peptide consensus to CbbL C-terminus and …

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. B. thetaiotaomicron mRNA toehold switch sensor

   Plasmid

   #110707

   Purpose

   Toehold switch sensor to detect a species specific mRNA with GFP output

   Depositor

   James Collins

   Article

   Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

   Insert

   B. thetaiotaomicron species specific toehold switch sensor
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-FLAG-eSpCas9

   Plasmid

   #126769

   Purpose

   Expression of increased fidelity eSpCas9 in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, MBP, and NLS

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. kank4_pJet1.2

   Plasmid

   #121982

   Purpose

   for generating kank4 riboprobes for WISH in zebrafish

   Depositor

   GuangJun Zhang

   Article

   Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

   Insert

   kank4 (kank4 Zebrafish)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA6-rbN1 S77A

   Plasmid

   #32945

   Depositor

   Edward Weinman

   Article

   Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

   Insert

   SLc9a3r1 (SLC9A3R1 rabbit)
   

   Use

   Tags

   T7 tag, his, and partial FLAG

   Expression

   Mammalian

   Mutation

   Change Serine77 to Alanine

   Promoter

   CMV

   Available since

   Feb. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCellFree_G03 TYK2

   Plasmid

   #67133

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TYK2 (TYK2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   A435S

   Promoter

   T7

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

8. TAL3080

   Plasmid

   #41254

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   ZebrafishCommunity-gata2b-Left (gata2b Zebrafish)
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

9. TAL3081

   Plasmid

   #41255

   Depositor

   Keith Joung

   Article

   Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

   Insert

   ZebrafishCommunity-gata2b-Right (gata2b Zebrafish)
   

   Use

   t7

   Tags

   3X Flag and WT FOKI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

10. TAL3105

    Plasmid

    #41279

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-irf6-Right (irf6 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

11. TAL3104

    Plasmid

    #41278

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-irf6-Left (irf6 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

12. TAL3071

    Plasmid

    #41245

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-ets1a-Right (ets1 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

13. TAL3070

    Plasmid

    #41244

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-ets1a-Left (ets1 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

14. TAL2473

    Plasmid

    #36854

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    CCND1-TALEN_Right (Ccnd1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

15. TAL2304

    Plasmid

    #36733

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    CCND1-TALEN_Left (Ccnd1 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

16. drd4a_R (TAL 3007)

    Plasmid

    #35999

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    TAL array targeting drd4a (drd4a Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TAL3403

    Plasmid

    #47122

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-apoeb-right (apoeb Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

18. TAL3402

    Plasmid

    #47121

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-apoeb-left (apoeb Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCellFree_G03 GTF2H4

    Plasmid

    #67094

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2H4 (GTF2H4 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCellFree_G03 MAPK13

    Plasmid

    #67077

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    MAPK13 (MAPK13 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pETM6-Pc4CL-PhCHS-CmCHI

    Plasmid

    #73397

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Pc4CL
    PhCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28b-NmCas9-Flag D16A+H588A

    Plasmid

    #109033

    Purpose

    D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Use

    Tags

    Flag Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pANY1

    Plasmid

    #110949

    Purpose

    Universal mini-vector for efficient cloning of DNA and expression of protein in E. coli.

    Depositor

    Yingfeng An

    Article

    Liu et al Biochem Biophys Res Commun. 2018 Mar 18;

    Insert

    ccd B cassette
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA6-rbN1 S77D

    Plasmid

    #32947

    Depositor

    Edward Weinman

    Article

    Weinman et al Am J Physiol Renal Physiol. 2009 Dec;297

    Insert

    slc9a3r1 (SLC9A3R1 rabbit)
    

    Use

    Tags

    T7 tag, his, and partial FLAG

    Expression

    Mammalian

    Mutation

    Serine 77 mutated to Aspartic Acid

    Promoter

    CMV

    Available since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Cwc25-pRSETA

    Plasmid

    #61043

    Purpose

    Encodes the yeast Cwc25 protein for overexpression and purification using a C-terminal hexahistidine tag. A single cysteine residue is encoded after the Cwc25 sequence for fluorescent labeling.

    Depositor

    Nils Walter

    Article

    Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

    Insert

    Cwc25
    

    Use

    Tags

    TEV site, Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

26. Ubiquitin (1-76; L71Y)

    Plasmid

    #110756

    Purpose

    Bacterial expression of human Ubiquitin (1-76; L71Y)

    Depositor

    David Komander

    Article

    Gladkova et al EMBO J. 2017 Dec 15;36(24):3555-3572. do

    Insert

    UBB (UBB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    L71Y

    Promoter

    T7

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TAL3420

    Plasmid

    #47139

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-def6a-left (def6a Zebrafish)
    

    Use

    T7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

28. HOXD13 (chicken) HIS-tag pET

    Plasmid

    #8555

    Depositor

    Corey Largman

    Article

    Shen et al J Biol Chem 1997 Mar 28;272(13):8198-206

    Insert

    HOXD13 (HOXD13 )
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    contains 10 nt 5' to ATG start; makes prote…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

29. TAL3219

    Plasmid

    #42682

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-atg5-right (atg5 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

30. TAL3217

    Plasmid

    #42680

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-atg12-right (atg12 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

31. TAL3218

    Plasmid

    #42681

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-atg5-left (atg5 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

32. TAL3216

    Plasmid

    #42679

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-atg12-left (atg12 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

33. pKV006

    Plasmid

    #64764

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, invariable V5 tag, Hygro selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFvL106

    Plasmid

    #64751

    Purpose

    Recombination-Induced Tag Exchange (RITE) Cassette, HA to a T7 epitope tag, URA selection

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    RITE cassette
    

    Use

    Rite cassette

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. TAL3315

    Plasmid

    #42778

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-meis2a-right (meis2.2 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

36. TAL3314

    Plasmid

    #42777

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-meis2a-left (meis2.2 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

37. pCellFree_G03 ATF3

    Plasmid

    #67057

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    ATF3 (ATF3 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    M2I

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

38. TAL3035

    Plasmid

    #41209

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-bsx-Right (bsx Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

39. TAL3043

    Plasmid

    #41217

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-cebpd-Right (cebpd Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

40. TAL3042

    Plasmid

    #41216

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-cebpd-Left (cebpd Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only