We narrowed to 9,370 results for: bli

1. p413TEF1-ATR1(pYL573)

   Plasmid

   #178288

   Purpose

   Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   ATR1
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

   Plasmid

   #174091

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Promoter

   pCMV and T7

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Hs.KRAS4b S17N

   Plasmid

   #83156

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S17N mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   S17N

   Available Since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. ACE2 g4 + Cas9

   Plasmid

   #153014

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

   Depositor

   Jason Sheltzer

   Insert

   Cas9 + ACE2 gRNA (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   TagBFP2

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Hs.KRAS4b Q61L

   Plasmid

   #83134

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Mutation

   Q61L

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ERAP1

   Plasmid

   #39174

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   ERAP1 (ERAP1 Human)
   

   Use

   Baculovirus expression

   Tags

   TEV-His10-Flag

   Promoter

   polyhedrin

   Available Since

   June 7, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pdx1-mTQ2-P2A-FlpO-PA

   Plasmid

   #67279

   Purpose

   Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context

   Depositor

   Jannik Elverløv-Jakobsen

   Inserts

   mTQ2=mturquoise2 blue fluorescent gene
   FlpO recombinase
   

   Tags

   linked to FlpO through an P2A ribosomal skipping …

   Expression

   Mammalian

   Promoter

   pdx

   Available Since

   July 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. CasRX_Pac

   Plasmid

   #194803

   Purpose

   CasRX vector for gene knockdown in Giardia. Insert gRNA using Bbs1 restriction site. Puromycin resistance cassette.

   Depositor

   Alexander Paredez

   Article

   Shih et al Proc Natl Acad Sci U S A. 2022 May 31;11

   Insert

   CasRX
   

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. ACE2 g2

   Plasmid

   #153012

   Purpose

   A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry

   Depositor

   Jason Sheltzer

   Insert

   ACE2 gRNA (ACE2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. C1QBP

    Plasmid

    #32863

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    C1QBP (C1QBP Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    amino acids E89-Q282 only

    Available Since

    Jan. 6, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pQTEV-RAB17

    Plasmid

    #34812

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    RAB17 (RAB17 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAG426-GAL-Alpha-synuclein-A53T

    Plasmid

    #181722

    Purpose

    Galactose inducible expression of Alpha-synuclein-A53T

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Expression

    Yeast

    Mutation

    A53T

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid

    #212122

    Purpose

    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    

    Tags

    His6 and SUMO

    Expression

    Bacterial

    Promoter

    araC and lpp

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBACgus N-His/GST/LRRK2 1867-2186

    Plasmid

    #40403

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    GST-Tev and HisTev

    Mutation

    WT aa1867-2186

    Promoter

    polH

    Available Since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. Hs.KRAS4b G13C

    Plasmid

    #83145

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13C mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G13C

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MECR

    Plasmid

    #38850

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MECR (Mecr Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

17. HSD17B4

    Plasmid

    #39156

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HSD17B4 (HSD17B4 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    codon-optimized

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

18. CD44I-c013

    Plasmid

    #175450

    Purpose

    Protein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220.

    Depositor

    Opher Gileadi

    Insert

    CD44 (CD44 Human)
    

    Tags

    His6

    Expression

    Mammalian

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Hs.KRAS4b Q61R

    Plasmid

    #83135

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61R

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO-Tet-On-HDAC1-shRNA2

    Plasmid

    #90241

    Purpose

    Lentivirus for expression of shRNA2 against human HDAC1 (Dox-inducible)

    Depositor

    Roland Friedel

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    Lentiviral

    Available Since

    May 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. XLone-Puro SARS-CoV2 N P2A eGFP-NLS

    Plasmid

    #154399

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFP

    Depositor

    Xiaoping Bao

    Insert

    SARS-CoV2 N Protein (N SARS-CoV2)
    

    Use

    Piggybac

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAG416-GAL-FUS

    Plasmid

    #181711

    Purpose

    Galactose inducible expression of FUS

    Depositor

    Alejandro Chavez

    Insert

    FUS (FUS Human)
    

    Expression

    Yeast

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. huntingtin_V2095-V3138_NbioCflag

    Plasmid

    #194765

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cells

    Depositor

    Cheryl Arrowsmith

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    Avi-tag (biotin acceptor peptide) and Flag-tag

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV and P10

    Available Since

    July 26, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pQTEV-STX6

    Plasmid

    #31581

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    syntaxin 6 (STX6 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pQE80L TriCatcher-ELP

    Plasmid

    #112630

    Purpose

    Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

    Depositor

    Mark Howarth

    Article

    Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

    Insert

    TriCatcher-ELP
    

    Tags

    His6 and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PTPN11

    Plasmid

    #38965

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    PTPN11 (PTPN11 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

27. Hs.KRAS4a G12V

    Plasmid

    #83169

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12V mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12V

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO-Tet-On-HDAC1-shRNA1

    Plasmid

    #90242

    Purpose

    Lentivirus for expression of shRNA1 against human HDAC1 (Dox-inducible)

    Depositor

    Roland Friedel

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    Lentiviral

    Available Since

    May 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pQTEV-PEPD

    Plasmid

    #31328

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    peptidase D (PEPD Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET3C-ARL16(CO)-His6

    Plasmid

    #216485

    Purpose

    Used for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.

    Depositor

    Richard Kahn

    Insert

    ADP-ribosylation factor-like 16 (ARL16 Human)
    

    Tags

    includes GSGSGSHHHHHH (His6) tag at C-terminus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. BASIC_SEVA_66.11

    Plasmid

    #194213

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 26, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. Hs.KRAS4a G12C

    Plasmid

    #83167

    Purpose

    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12C mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    G12C

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pOTTC533 - pAAV SYN1 eGFP-2A-mKv1.2

    Plasmid

    #75295

    Purpose

    An AAV packaging vector that expresses eGFP and Kv1.2 under the control of a neuronal promoter.

    Depositor

    Brandon Harvey

    Insert

    eGFP-2A-mKv1.2 (Kcna2 Mouse, Synthetic)
    

    Use

    AAV

    Promoter

    human SYN1

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Lamin A (R527P)-mRFP

    Plasmid

    #124272

    Purpose

    Lamin A mutant (R527P), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.

    Depositor

    Eric Schirmer

    Insert

    LMNA (R527P) (LMNA Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    LMNA (R527P)

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pExo1

    Plasmid

    #53357

    Purpose

    Expresses beta-lactamase for the degradation of beta-lactam antibiotics and tags it with hlyAs (transporter gene) in preparation for secretion from the cell.

    Depositor

    Lingchong You

    Article

    Pai et al Proc Natl Acad Sci U S A. 2012 Nov 27;10

    Inserts

    BlaMs
    hlyAs
    

    Promoter

    p(lac/ara-1)

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. HSP90B1

    Plasmid

    #39076

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    HSP90B1 (HSP90B1 Human)
    

    Tags

    TEV-His6-Flag

    Expression

    Bacterial

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

37. p-dCas9-DNMT3a-Hygro

    Plasmid

    #104403

    Purpose

    transient expression of dCas9-DNMT3a fusion protein

    Depositor

    Stephan Beck

    Insert

    DNMT3a (DNMT3A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Lamin A (R482Q)-mRFP

    Plasmid

    #124273

    Purpose

    Lamin A mutant (R482Q), C-terminally tagged with mRFP. Mutation associated with FPLD lipodystrophy.

    Depositor

    Eric Schirmer

    Insert

    LMNA (R482Q) (LMNA Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    LMNA (R482Q)

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. BRAF - A12G in pENTR

    Plasmid

    #216606

    Purpose

    gateway cloning donor vector containing BRAF ASD mutation

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    BRAF (BRAF Human)
    

    Use

    Gateway entry plasmid

    Mutation

    A12G

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pET28-H12TEV-hEIF5B-1~1220(FL)

    Plasmid

    #208636

    Purpose

    for E. coli expression of human eIF5B (ENST00000289371)

    Depositor

    Quanfu Ma

    Insert

    human eIF5B (ENST00000289371) (EIF5B Human)
    

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Contain SNP rs1376443, protein sequence not chang…

    Promoter

    T7

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge