We narrowed to 17,776 results for: REV

1. miniTol2 x EF1a_EKAREN4 + PGK-puro

   Plasmid

   #167824

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. miniTol2 x EF1a_EKAREN4 + PGK-blast

   Plasmid

   #167825

   Purpose

   Optimized EKAREV FRET biosensor sensor for ERK

   Depositor

   Hugo Snippert

   Article

   Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

   Insert

   EKAREN4
   

   Use

   Tags

   nls localization motif

   Expression

   Mammalian

   Mutation

   K424P, K426W

   Promoter

   EF1a

   Available since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pZE21/UBP1/ClpS_V65I

   Plasmid

   #98567

   Purpose

   Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon

   Depositor

   George Church

   Article

   Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

   Inserts

   Truncated ubiquitin cleavase, codon optimized
   Mutated ATP-dependent Clp protease adapter protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains the V65I mutation that increases discrim…

   Promoter

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

   Plasmid

   #91820

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   10xHis

   Expression

   Yeast

   Mutation

   5xGly linker, PreScission Protease site (LEVLFQGP…

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCag SE (Self-excising) FlpO-2A-Cre EV

   Plasmid

   #130986

   Purpose

   episomal expression of FlpO and Cre recombinases, self excising

   Depositor

   Joshua Breunig

   Article

   Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

   Insert

   FlpO-2A-Cre
   

   Use

   Cre/Lox and Unspecified; Episomal expression vect…

   Tags

   Expression

   Mammalian

   Mutation

   protamine intron added to FlpO to prevent bacteri…

   Promoter

   CMV/Chick β-actin (CAG)

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS426-HsFECH

   Plasmid

   #174524

   Purpose

   Expresses human ferrochelatase (FECH) in yeast

   Depositor

   Oleh Khalimonchuk

   Article

   Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

   Insert

   FECH with yeast HEM15 promoter and terminator (FECH Budding Yeast, Human, Synthetic)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Lacks original mitochondrial targeting sequence (…

   Promoter

   HEM15 (yeast, 257 bp)

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJOP-HTT-HR65Q

   Plasmid

   #92249

   Purpose

   HTT donor DNA with 65Q for homologous recombination-mediated gene modification

   Depositor

   Mahmoud Pouladi

   Article

   Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

   Insert

   HTT donor DNA (HTT Human)
   

   Use

   Precisionx hr targeting vector

   Tags

   Expression

   Mutation

   Barcode of wobble bases to prevent TALEN cleaving…

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJOP-HTT-HR45Q

   Plasmid

   #92248

   Purpose

   HTT donor DNA with 45Q for homologous recombination-mediated gene modification

   Depositor

   Mahmoud Pouladi

   Article

   Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

   Insert

   HTT donor DNA (HTT Human)
   

   Use

   Precisionx hr targeting vector

   Tags

   Expression

   Mutation

   Barcode of wobble bases to prevent TALEN cleaving…

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJOP-HTT-HR81Q

   Plasmid

   #92250

   Purpose

   HTT donor DNA with 81Q for homologous recombination-mediated gene modification

   Depositor

   Mahmoud Pouladi

   Article

   Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

   Insert

   HTT donor DNA (HTT Human)
   

   Use

   Precisionx hr targeting vector

   Tags

   Expression

   Mutation

   Barcode of wobble bases to prevent TALEN cleaving…

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJOP-HTT-HR30Q

    Plasmid

    #92247

    Purpose

    HTT donor DNA with 30Q for homologous recombination-mediated gene modification

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    HTT donor DNA (HTT Human)
    

    Use

    Precisionx hr targeting vector

    Tags

    Expression

    Mutation

    Barcode of wobble bases to prevent TALEN cleaving…

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUC57-BBclone1

    Plasmid

    #137069

    Purpose

    E. coli plasmid that contains the expression optimized genes for BB0405, BB0406, P66, BBA57, HtrA, RevA and BB0238

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Inserts

    BB0405 (BB_0405 Borrelia burgdorferi B31)
    BB0406
    P66
    BBA57
    HtrA
    RevA (revA Borrelia burgdorferi B31)
    BB0238
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    wild-type, full-length (signal-peptide-containing…

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2+HyPer7-NES

    Plasmid

    #136467

    Purpose

    Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Nuclear export signal from the HIV Rev protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJH143

    Plasmid

    #68382

    Purpose

    reverse IMCE donor plasmid

    Depositor

    Trevor Charles

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pET29b-Cwc25

    Plasmid

    #111341

    Purpose

    his tagged Cwc25

    Depositor

    Christine Guthrie

    Insert

    CWC25
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28B-Cwc25

    Plasmid

    #111340

    Purpose

    his tagged Cwc25

    Depositor

    Christine Guthrie

    Insert

    CWC25
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pOME0294M

    Plasmid

    #182623

    Purpose

    KSHV anti-RTA_3Kb_Reverse

    Depositor

    Dirk Dittmer

    Insert

    KSHV anti-RTA_3Kb_Reverse
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion at 2853

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS316-Cwc25

    Plasmid

    #111343

    Purpose

    Cwc25 in pRS316(Ura) vector

    Depositor

    Christine Guthrie

    Insert

    CWC25
    

    Use

    Tags

    none

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYBS187

    Plasmid

    #18901

    Depositor

    Elaine Elion

    Insert

    Cdc28 (CDC28 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pAG25-1199 cDNA

    Plasmid

    #160379

    Purpose

    N1199 narnavirus cDNA

    Depositor

    John McCusker

    Insert

    N1199
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    NA

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOME0292M

    Plasmid

    #182621

    Purpose

    PAN-RNA_RevHuman herpesvirus 8 strain JSC-1 clone BAC16, complete genome.

    Depositor

    Dirk Dittmer

    Insert

    Pan_RNA_Rev
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge