We narrowed to 18,420 results for: REV

1. pEasyG2_zeo

   Plasmid

   #184913

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEasyG2_nat

   Plasmid

   #184914

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEasyG3_zeo

   Plasmid

   #184917

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET151-Vps4(101-437)-Hcp1 K321A/R325A

   Plasmid

   #89287

   Purpose

   Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Tags

   6xHis-V5-TEV and Hcp1

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100, mutated Lys-321 and Ar…

   Promoter

   T7

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. M7138

   Plasmid

   #219774

   Purpose

   MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of ORCA3 promoter and terminator

   Depositor

   Karen Sarkisyan

   Article

   Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

   Insert

   pNOS-HygR-ocsT | pORCA3-nnLuz-ORCA3_T
   

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   pNOS-HygR-ocsT | pORCA3-nnLuz-ORCA3_T

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. M7691

   Plasmid

   #219775

   Purpose

   MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of WRKY70 promoter and terminator

   Depositor

   Karen Sarkisyan

   Article

   Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

   Insert

   pNOS-HygR-ocsT | pWRKY70-nnLuz-WRKY70_T
   

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   pNOS-HygR-ocsT | pWRKY70-nnLuz-WRKY70_T

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNK3558

   Plasmid

   #219776

   Purpose

   MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of p35S promoter and act2 terminator

   Depositor

   Karen Sarkisyan

   Article

   Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

   Insert

   pNOS-HygR-ocsT | p35S-nnLuz-act2
   

   Use

   Synthetic Biology

   Expression

   Plant

   Promoter

   pNOS-HygR-ocsT | p35S-nnLuz-act2

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCas3-APOBEC1

   Plasmid

   #229536

   Purpose

   pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   rAPOBEC1
   Cas3
   Uracil glycosylase inhibitor
   

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15

   Plasmid

   #207054

   Purpose

   Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

   Depositor

   Zhiping Xie

   Article

   He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

   Insert

   ATG8
   

   Tags

   3xVSV

   Expression

   Yeast

   Mutation

   L55W T56A V61W R65A

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4.0-TERT_-100_-79

    Plasmid

    #193785

    Purpose

    luciferase reporter for duplicated TERT promoter

    Depositor

    Joseph Costello

    Article

    Barger et al Nat Commun. 2022 Sep 16;13(1):5430. doi:

    Insert

    TERT promoter (TERT Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    TERT

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2

    Plasmid

    #185346

    Purpose

    Membrane anchoring of UBE2G2. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    Cue1p/Ube2G2 fusion (internal MYC tag)
    

    Expression

    Mammalian

    Available Since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)-Cue1pTM-MYC-UBE2G2-FLAG

    Plasmid

    #185347

    Purpose

    Membrane anchoring of UBE2G2 and co-IP. Encodes transmembrane domain of S. Cerevisiae Cue1p fused to MYC-UBE2G2 with C-terminal FLAG tag

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    Cue1p/Ube2G2 fusion (internal MYC tag)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIALD2HMGr

    Plasmid

    #185867

    Purpose

    Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CPH3486 (YCp LEU2 Rpb1 F1492A)

    Plasmid

    #91808

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with a F1492A mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    changed phenylalanine 1492 to alanine

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CPH3487 (YCp LEU2 Rpb1 S1493A)

    Plasmid

    #91809

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with a S1493A mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    changed serine 1493 to alanine

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CPH3488 (YCp LEU2 Rpb1 P1494A)

    Plasmid

    #91810

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with a P1494A mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    changed proline 1494 to alanine

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CPH3508 (YCp LEU2 Rpb1 Y1473A)

    Plasmid

    #91811

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with a Y1473A mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    changed tyrosine 1473 to alanine

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CPH3509 (YCp LEU2 Rpb1 T1471A)

    Plasmid

    #91812

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with a T1471A mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    changed threonine 1471 to alanine

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CPH3511 (YCp LEU2 Rpb1 P1472A)

    Plasmid

    #91813

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with a P1472A mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    changed proline 1472 to alanine

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CPH3567 (pET151_Spt6 1247-1451 R1282H)

    Plasmid

    #91816

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed arginine 1282 to histidine; deleted resid…

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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