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6xHis-SNAP-PI(3,4,5)P3 Probe

Plasmid

#211514

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-3,4,5-Trisphosphate (PI(3,4,5)P3) from bacteria

Depositor

Hannes Maib, David Murray

Publication

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

BTK1-PH

Tags

6xhis-SNAP

Expression

Bacterial

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pIW601-KmCRISPR

Plasmid

#98907

Purpose

K. marxianus CRISPR Plasmid for sgRNA cloning

Depositor

Ian Wheeldon

Publication

Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

Inserts

Codon optimized Cas9
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Tags

SV40

Expression

Yeast

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5-FRT-GFP-mCherry-3pGW

Plasmid

#53965

Purpose

Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

Depositor

Saeed Tavazoie

Publication

Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

Inserts

GFP
mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pW34

Plasmid

#158210

Purpose

LuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites.

Depositor

Yasuo Yoshikuni

Publication

Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

Insert

LuxABCDE

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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lenti-mTagBFP2

Plasmid

#216126

Purpose

Lentiviral vector for expressing mTagBFP2.

Depositor

Stefan Kubicek

Publication

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-mTagBFP2-WPRE

Use

Lentiviral

Expression

Mammalian

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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PLVX_SPTA1_IGF2BP1_IRES_PURO

Plasmid

#100016

Purpose

To over-express IGF2BP1 in Adult Erythroblasts using tissue specific promoter with IRES for puromycin selection.

Depositor

Jeffery Miller

Publication

de Vasconcellos et al Proc Natl Acad Sci U S A. 2017 Jul 11;11

Insert

Insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SPTA1 erythroid specific promoter

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p6345 MSCV-CMV-Flag-HA-Brd4 1-722

Plasmid

#31352

Depositor

Peter Howley

Publication

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4 1-722 (BRD4 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

*see comments

Available since

Oct. 24, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-link-yETSapphire-CaUra3

Plasmid

#44650

Depositor

Kurt Thorn

Insert

Turbo Sapphire

Expression

Yeast

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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JNK-KTR-mStrawberry reporter

Plasmid

#158689

Purpose

This plasmid is a JNK pathway reporter. It has CMV promoter driving the expression of JNK-KTR fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Publication

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pAIP hIL4 co

Plasmid

#74169

Purpose

Lentiviral vector for expression of human codon optimized IL4 with puromycin resistance.

Depositor

Jeremy Luban

Publication

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

IL4 (IL4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pSGNluc

Plasmid

#186705

Purpose

A high-sensitivity bi-directional reporter to monitor NF-κB activity in cell culture and zebrafish in real time

Depositor

Baubak Bajoghli, Maria Leptin

Publication

Kuri et al J Cell Sci. 2017 Feb 1;130(3):648-657. d

Inserts

GFP
luciferase

Available since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pBIG2ab

Plasmid

#80616

Purpose

pBIG2ab - Step2 vector ab (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Publication

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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PCT62

Plasmid

#170391

Purpose

expresses three sgRNA cassettes

Depositor

Jonathan Weissman

Publication

Quinn et al Science. 2021 Feb 26;371(6532). pii: sci

Insert

PCT62-targetsite

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B Flag BimEL

Plasmid

#23090

Depositor

Roger Davis

Publication

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bim EL (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pPac Sp1

Plasmid

#12095

Depositor

Robert Tjian

Publication

Courey et al Cell. 1988 Dec 2. 55(5):887-98.

Insert

Sp1 (SP1 Human)

Expression

Insect

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-LACTB-dAPEX2

Plasmid

#117179

Purpose

Plasmid name in publication: pAAV-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Publication

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

LACTB-dAPEX2

Use

AAV

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MGAT-SEP-mRuby3

Plasmid

#200937

Purpose

cis/medial golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

Depositor

Nir Hacohen

Publication

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Inserts

MGAT2 (MGAT2 Human)
SEP-mRuby3

Use

Lentiviral

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHACK-GAL4>QF2

Plasmid

#80275

Purpose

GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.

Depositor

Christopher Potter

Publication

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Expression

Insect

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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F-MED26

Plasmid

#15367

Depositor

Joan Conaway, Ronald Conaway

Publication

Sato et al Mol Cell. 2004 Jun 4. 14(5):685-91.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MED26 (MED26 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
p5E-hPGK

Plasmid

#82579

Purpose

5' entry vector containing human phosphoglycerate kinase (hPGK) promoter for ubiquitous expression

Depositor

Kryn Stankunas

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

Human PGK promoter

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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