We narrowed to 9,591 results for: EPO

1. pFBOH-MHL.RING1B

   Plasmid

   #224712

   Purpose

   Expresses N-terminal hexahistidine-tagged human RING1B (RNF2) in insect cells.

   Depositor

   Chen Davidovich

   Article

   Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

   Insert

   RING1b (RNF2 Human)
   

   Use

   Tags

   6x histidine tag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Oct. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pFBOH-MHL.BMI1

   Plasmid

   #224711

   Purpose

   Expresses N-terminal hexahistidine-tagged human BMI1 (PCGF4) in insect cells.

   Depositor

   Chen Davidovich

   Article

   Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

   Insert

   BMI1 (BMI1 Human)
   

   Use

   Tags

   6x histidine tag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCL025 AAVS1-9xTetO-RSV-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #188743

   Purpose

   Donor vector for integrating RSV promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Surface marker and mCitrine reporter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCL026 AAVS1-9xTetO-hPGK-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #188744

   Purpose

   Donor vector for integrating PGK promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Surface marker and mCitrine reporter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #188745

   Purpose

   Donor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Surface marker and mCitrine reporter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   nonTATAchr21

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCL053 AAVS1-9xTetO-nTATAchrX-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

   Plasmid

   #188746

   Purpose

   Donor vector for integrating nonTATAchrX promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   Surface marker and mCitrine reporter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   nonTATAchrX

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PCRbluntII-pHES7-STOPgRNA

   Plasmid

   #204349

   Purpose

   gRNA to target pig HES7 stop codon

   Depositor

   Li-Fang Chu

   Article

   Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

   Insert

   Pig HES7-STOP-gRNA (HES7 Sus domesticus (pig))
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-noSV40-EC1.45-human_min1-opossum_min2

   Plasmid

   #173966

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   Human minimal enhancer region 1 (min1) and opossum minimal enhancer region 2 (min2) from SOX9 enhancer cluster EC1.45
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   See Depositor Comments Below

   Promoter

   SV40 promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. RG6-BAP1_E7-D192D

   Plasmid

   #154024

   Purpose

   Bichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 7 c.576C>T, p.D192D (Synonymous Mutation)

   Depositor

   Samuel Peña-Llopis

   Article

   Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

   Insert

   BAP1 Exon 7 Fused to DsRed1 and eGFP (BAP1 Human)
   

   Use

   Splicing reporter

   Tags

   DsRed1, FLAG, SV40 NLS, and eGFP

   Expression

   Mammalian

   Mutation

   BAP1 c.576C>T, p.D192D

   Promoter

   Available since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. RG6-BAP1_E11-G312G

    Plasmid

    #154026

    Purpose

    Bichromatic Fluorescent Splicing Reporter Minigene for Mutant BAP1 Exon 11 c.936T>G, p.G312G (Synonymous Mutation)

    Depositor

    Samuel Peña-Llopis

    Article

    Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

    Insert

    BAP1 Exon 11 Fused to DsRed1 and eGFP (BAP1 Human)
    

    Use

    Splicing reporter

    Tags

    DsRed1, FLAG, SV40 NLS, and eGFP

    Expression

    Mammalian

    Mutation

    BAP1 c.936T>G, p.G312G

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCJ200

    Plasmid

    #162673

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C and a sequence Tyr75:Phe104 to introduce a 6th disulfide bond as in AoFaeB-2.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    S136C and a sequence Y75-F104 to introduce a 6th…

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCJ210

    Plasmid

    #162683

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S136C, G489C, S530C, and a sequence Tyr75:Phe104 to introduce a 6th and 7th disulfide bond as in AoFaeB-2.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    S136C, G489C, S530C, and a sequence Y75-F104 to i…

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCJ220

    Plasmid

    #162686

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224W and C529S mutations from PETase active site.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with lid deletion and C224W and C529S mutations
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaG251-T472, C224W, C529S; codon optimized for…

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCJ221

    Plasmid

    #162687

    Purpose

    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating lid deletion (Gly251:Thr472) and C224H and C529F mutations from PETase active site.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with lid deletion and C224H and C529F mutations
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    deltaG251-T472, C224H, C529F; codon optimized for…

    Promoter

    T7/lac

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmTHC_PGKneoLox2DTA.2

    Plasmid

    #112624

    Purpose

    H2BmTeal_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-mTFP1 (H2BC3 Human, Anthozoa)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    mTeal (mTFP1)

    Expression

    Mutation

    Promoter

    No Promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEHC_PGKneoLox2DTA.2

    Plasmid

    #112623

    Purpose

    H2BEmerald_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

    Depositor

    Stefan Heller

    Article

    Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

    Inserts

    H2B-Emerald (H2BC3 Aequorea victoria, Human)
    Hygrophosphotransferase
    Cre-ERT2 fusion protein
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Emerald

    Expression

    Mutation

    Promoter

    No Promoter

    Available since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMVtrunc beta-actinh7TAA-msfGFPΔN7ΔC11-SSSSactin

    Plasmid

    #231549

    Purpose

    Mammalian expression of human β-actin fused intramolecularly to N- and C-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    ACTB (ACTB Human)
    

    Use

    Tags

    msfGFP (intramolecular)

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtrunc

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMVtrunc msfGFPΔC10-beta-actin

    Plasmid

    #231548

    Purpose

    Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    ACTB (ACTB Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtrunc

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Ai162 (TIT2L-GC6s-ICL-tTA2) targeting vector

    Plasmid

    #114433

    Purpose

    Target a Cre-dependent GCaMP6s cassette and a tTA2 cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    GCaMP6s, tTA2
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    TRE2, CAG

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

    Plasmid

    #112669

    Purpose

    Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.

    Depositor

    Catherine Verfaillie

    Article

    Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

    Insert

    tdT
    

    Use

    AAV; Donor plasmid for recombinase-mediated casse…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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