We narrowed to 8,371 results for: BLI;

1. MAGI1

   Plasmid

   #38882

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MAGI1 (MAGI1 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   C890S; codon-optimized

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

2. MAGI1

   Plasmid

   #38883

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MAGI1 (MAGI1 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   C1189S; codon-optimized

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

3. MAGI1

   Plasmid

   #38901

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MAGI1 (MAGI1 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   C677S, C709S; codon-optimized

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. DMTF1

   Plasmid

   #36895

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   DMTF1 (DMTF1 Human)
   

   Use

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   contains Myb-like 1 domain, residues 220-274

   Promoter

   Available since

   June 19, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pTipi2.1_Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa

   Plasmid

   #221360

   Purpose

   Mammalian expression of Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa; to be used with Anti-V5 [IPI-SV5-Pk1] heavy chain (Plasmid 221359) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-V5 [IPI-SV5-Pk1] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. FUW-tetO-NFIA

   Plasmid

   #141403

   Purpose

   Inducible Expression of NFIA

   Depositor

   Lorenz Studer

   Article

   Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

   Insert

   NFIA (NFIA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. eGFP-hCOL1A1

   Plasmid

   #140110

   Purpose

   Expresses fluorescently-tagged human Type I procollagen α1 chain with eGFP replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

   Depositor

   Sergey Leikin

   Insert

   Type I procollagen α1 (COL1A1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   COL1A1 exons 2-3 replaced by fluorescent tag, K20…

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTipi2.1_Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit HC

   Plasmid

   #221363

   Purpose

   Mammalian expression of Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit HC; to be used with Anti-His [IPI-HAP1/5.13.22] light chain (Plasmid 221364) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pTipi2.1_Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa

   Plasmid

   #221364

   Purpose

   Mammalian expression of Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa; to be used with Anti-His [IPI-HAP1/5.13.22] heavy chain (Plasmid 221363) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-His [IPI-HAP1/5.13.22] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. AICSDP-47: CTNNB1-mEGFP

    Plasmid

    #109119

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human CTNNB1

    Depositor

    Allen Institute for Cell Science

    Insert

    CTNNB1 Homology Arms with mEGFP-linker (CTNNB1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    May 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pOTTC1740 - pAAV SYN1 DIO HA-hM4D(Gi)

    Plasmid

    #125146

    Purpose

    An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter

    Depositor

    Christopher Richie

    Insert

    HA-tagged hM4D(Gi) (CHRM4 Human)
    

    Use

    AAV and Cre/Lox

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    SYN1

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJMP1358

    Plasmid

    #119277

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DT960

    Plasmid

    #80412

    Purpose

    Expresses 960 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. DT480

    Plasmid

    #80413

    Purpose

    Expresses 480 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTipi2.1_Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa

    Plasmid

    #221356

    Purpose

    Mammalian expression of Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa; to be used with Anti-Strep [IPI-C23.21] heavy chain (Plasmid 221355) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pCDH-EF1-FHC-POLQ

    Plasmid

    #64875

    Purpose

    lentiviral expression of human POLQ

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Aug. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDONR223_LacZ

    Plasmid

    #25893

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    LacZ
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLJM1 EGFP-Tau

    Plasmid

    #108868

    Purpose

    Expresses EGFP linked to human Tau

    Depositor

    Gabriele Kaminski Schierle

    Insert

    Tau (MAPT Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    human four-repeat tau lacking the N-terminal sequ…

    Promoter

    CMV

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTipi2.1_Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa

    Plasmid

    #221358

    Purpose

    Mammalian expression of Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa; to be used with Anti-DYKDDDDK [IPI-4E11/M1] heavy chain (Plasmid 221357) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-DYKDDDDK [IPI-4E11/M1] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pDONR223_BFP

    Plasmid

    #25891

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    BFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Tandem p40p35

    Plasmid

    #108665

    Purpose

    Expression of bioactive mouse IL-12 as a tandem dimer of p40 and p35 linked with a Gly-Ser linker

    Depositor

    Nevil Singh

    Insert

    interleukin-12 (Il12a, Il12b Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    IL-12 dimers have a Gly-Ser linker

    Promoter

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCMV-Spike-VLP

    Plasmid

    #173922

    Purpose

    Expresses virus-like particles based upon the original SARS-Cov-2

    Depositor

    Andreas Stuermer

    Inserts

    SARS-2 Spike Protein (S SARS-COV-2, Human, Synthetic)
    SARS-Cov-2 E protein (E SARS-COV-2, Human, Synthetic)
    SARS-Cov-2 M Protein (M SARS-COV-2, Human, Synthetic)
    

    Use

    Tags

    no tags, native protein

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    22bp IRES, CMV, and IE2 Promoter/Enhancer from Mo…

    Available since

    April 11, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. loxP-G418-LoxP-TurboID-Rab11 HR

    Plasmid

    #230026

    Purpose

    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with TurboID and a V5 epitope tag. Contains a G418 resistance cassette for selection of edited cells

    Depositor

    Francesca Bottanelli

    Insert

    genomic sequence Rab11 locus (RAB11A Human)
    

    Use

    CRISPR

    Tags

    Turbo-ID, V5

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. K44A dynamin 1 pEGFP

    Plasmid

    #34681

    Depositor

    Sandra Schmid

    Article

    Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

    Insert

    dynamin 1ba (DNM1L Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDONR221_EGFP

    Plasmid

    #25899

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTipi2.1_Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa

    Plasmid

    #221366

    Purpose

    Mammalian expression of Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa; to be used with Anti-Rho IPI-[1D4] heavy chain (Plasmid 221365) to make the antibody.

    Depositor

    Institute for Protein Innovation (IPI)

    Insert

    Anti-Rho [IPI-1D4] Mouse/Rabbit LC kappa
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. AAVS1-tdTomato

    Plasmid

    #159275

    Purpose

    Human AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Proc Natl Acad Sci U S A. 2018 Apr 10;11

    Insert

    tdTomato
    

    Use

    AAV; S1 knockin donor vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

    Plasmid

    #174081

    Purpose

    Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

    Depositor

    Ryan Mehl

    Article

    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

    Insert

    NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
    

    Use

    Tags

    NES-Flag

    Expression

    Mammalian

    Mutation

    L270G, Y271L, N311G, C313A

    Promoter

    CMV

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid

    #185914

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HaloTag-beta2-adrenergic receptor

    Plasmid

    #66994

    Purpose

    Beta-2-adrenergic receptor tagged with HaloTag on amino terminus

    Depositor

    Catherine Berlot

    Insert

    HaloTag-beta2-adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    Human Growth hormone Signal Sequence-HaloTag

    Expression

    Mammalian

    Mutation

    The signal sequence from human growth hormone was…

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Lenti:FlipGFP-T2A-mCherry

    Plasmid

    #223198

    Purpose

    Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

    Depositor

    Yeh-Hsing Lao

    Article

    Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

    Insert

    FlipGFP-T2A-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLX303

    Plasmid

    #25897

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AICSDP-27:SLC25A17-mEGFP

    Plasmid

    #101784

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SLC25A17

    Depositor

    Allen Institute for Cell Science

    Insert

    SLC25A17 Homology Arms with linker-mEGFP (SLC25A17 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Oct. 31, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pcDNAintron-XKR8-n3xFLAG

    Plasmid

    #215693

    Purpose

    Produces the XKR8 lipid scramblase with an n-terminal 3xFLAG tag

    Depositor

    Melinda Brindley

    Article

    Acciani et al J Virol. 2021 Sep 27;95(20):e0116521. do

    Insert

    XKR8-n3xFLAG (XKR8 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pQTEV-ASF1A

    Plasmid

    #31591

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AICSDP-28:TUBA1B-mTagRFP-T

    Plasmid

    #101785

    Purpose

    Homology arms and mTagRFPT-linker sequence for N-terminus (second exon) tagging of human TUBA1B

    Depositor

    Allen Institute for Cell Science

    Insert

    TUBA1B Homology Arms with mTagRFPT-linker (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    mTagRFPT-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Oct. 31, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pJMP1237

    Plasmid

    #119262

    Purpose

    for cloning new sgRNAs

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AICSDP-41: ATP2A2-mEGFP

    Plasmid

    #114405

    Purpose

    Homology arms and mEGFP-linker sequence for N-terminus tagging of human ATP2A2

    Depositor

    Allen Institute for Cell Science

    Insert

    ATP2A2 Homology Arms with mEGFP-linker (ATP2A2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available since

    Sept. 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pEC3006 (pSpy1C-PZn_ffluc)

    Plasmid

    #218515

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PZn (PczcD)

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. Lenti-UbC-FKRP-EF1a-BSD

    Plasmid

    #205150

    Purpose

    Express UbC-driven FKRP and EF-1a driven BSD

    Depositor

    Monkol Lek

    Insert

    Fukutin-related protein (FKRP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge