Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,474 results for: BLI;

Clear keywords
Showing: 7001 - 7040 of 9474 results

  1. CAMK2G

    Plasmid
    #38894
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CAMK2G (CAMK2G Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. p-dCas9-p300-Hygro

    Plasmid
    #104411
    Purpose
    transient expression of dCas9-p300 fusion protein
    Depositor
    Stephan Beck
    Insert
    p300 (EP300 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104411-plasmid-map-sequence-id-204503

    Enlarge

  5. pAG426-GAL-Alpha-synuclein-H50Q

    Plasmid
    #181724
    Purpose
    Galactose inducible expression of Alpha-synuclein-H50Q
    Depositor
    Alejandro Chavez
    Insert
    Alpha-synuclein (SNCA Human)
    Expression
    Yeast
    Mutation
    H50Q
    Available Since
    April 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    181724-plasmid-map-sequence-id-431239

    Enlarge

  6. pAAV2-CAGIG-LAMP1-mGL

    Plasmid
    #222394
    Purpose
    To express in mammalian cells, the lysosome-associated membrane glycoprotein 1 (LAMP1) fused with the FP protein mGreenLantern. This plasmid used to label lysosomes in transfected and infected cells.
    Depositor
    Pantelis Tsoulfas
    Insert
    LAMP1 and mGreenlantern (LAMP1 Homo Sapiens, Synthetic, Human)
    Use
    AAV
    Tags
    LAMP1 and mGreenLantern
    Expression
    Mammalian
    Promoter
    CAG or CAGIG
    Available Since
    Sept. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    222394-plasmid-map-sequence-id-446519

    Enlarge

  7. DOT1L

    Plasmid
    #36196
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (GeneScript Synthesized) (DOT1L Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains amino acid residues 1-420
    Available Since
    Aug. 8, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. DOT1L

    Plasmid
    #40736
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DOT1L (DOT1L Human)
    Tags
    6xHis TEV
    Expression
    Bacterial
    Available Since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. pLKO.1-blast shKGA

    Plasmid
    #110420
    Purpose
    shKGA, silence glutaminase KGA isoform, blasticidin selection.
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    U6 (RNA Pol III)
    Available Since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110420-plasmid-map-sequence-id-213695

    Enlarge

  10. CD44Z-c011

    Plasmid
    #175455
    Purpose
    Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Tags
    His6
    Expression
    Mammalian
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175455-plasmid-map-sequence-id-353216

    Enlarge

  11. Hs.KRAS4b G12R

    Plasmid
    #83143
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12R mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    G12R
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83143-plasmid-map-sequence-id-158106

    Enlarge

  13. pBabe-Kras G12V-T35S

    Plasmid
    #58900
    Purpose
    Expresses human Kras with G12V and T35S mutations
    Depositor
    Channing Der
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    G12V and T35S mutations (effector binding mutant)
    Available Since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  15. Hs.KRAS4b S17N

    Plasmid
    #83156
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S17N mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    S17N
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83156-plasmid-map-sequence-id-158119

    Enlarge

  16. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

    Plasmid
    #174091
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Promoter
    pCMV and T7
    Available Since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174091-plasmid-map-sequence-id-341917

    Enlarge

  17. pdx1-mTQ2-P2A-FlpO-PA

    Plasmid
    #67279
    Purpose
    Pancreas specific expression of the FlpO recombinase controlled by the pdx1 promoter . FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Depositor
    Jannik Elverløv-Jakobsen
    Inserts
    mTQ2=mturquoise2 blue fluorescent gene
    FlpO recombinase
    Tags
    linked to FlpO through an P2A ribosomal skipping …
    Expression
    Mammalian
    Promoter
    pdx
    Available Since
    July 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    67279-plasmid-map-sequence-id-115888

    Enlarge

  18. ERAP1

    Plasmid
    #39174
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ERAP1 (ERAP1 Human)
    Use
    Baculovirus expression
    Tags
    TEV-His10-Flag
    Promoter
    polyhedrin
    Available Since
    June 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. pTP-AAV-ChATen-mCherry

    Plasmid
    #193741
    Purpose
    This AAV vector contains a ChAT enhancer that drives mCherry expression in spinal cord skeletal motor neurons. Specificity of vector not characterized outside the mouse spinal cord.
    Depositor
    Hynek Wichterle
    Insert
    ChAT enhancer, mini promoter, synthetic intron, mCherry
    Use
    AAV and Mouse Targeting
    Expression
    Mammalian
    Promoter
    1000 bp ChAT enhancer with a 32 bp minimal promot…
    Available Since
    Oct. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    193741-plasmid-map-sequence-id-450307

    Enlarge

  21. ACE2 g4 + Cas9

    Plasmid
    #153014
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    Lentiviral
    Tags
    TagBFP2
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153014-plasmid-map-sequence-id-298677

    Enlarge

  22. Hs.KRAS4b Q61L

    Plasmid
    #83134
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    Q61L
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83134-plasmid-map-sequence-id-158097

    Enlarge

  23. ACE2 g2

    Plasmid
    #153012
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    ACE2 gRNA (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153012-plasmid-map-sequence-id-298678

    Enlarge

  24. C1QBP

    Plasmid
    #32863
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    C1QBP (C1QBP Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    amino acids E89-Q282 only
    Available Since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  27. HSD17B4

    Plasmid
    #39156
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSD17B4 (HSD17B4 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. Hs.KRAS4b G13C

    Plasmid
    #83145
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G13C mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    G13C
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83145-plasmid-map-sequence-id-158108

    Enlarge

  30. XLone-Puro SARS-CoV2 N P2A eGFP-NLS

    Plasmid
    #154399
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154399-plasmid-map-sequence-id-304590

    Enlarge

  33. Hs.KRAS4a G12V

    Plasmid
    #83169
    Purpose
    Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12V mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    G12V
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83169-plasmid-map-sequence-id-158132

    Enlarge

  34. CD44I-c013

    Plasmid
    #175450
    Purpose
    Protein expression/secretion in mammalian cells. CD44 Link module (exons 2-5), A20-P220.
    Depositor
    Opher Gileadi
    Insert
    CD44 (CD44 Human)
    Tags
    His6
    Expression
    Mammalian
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175450-plasmid-map-sequence-id-353226

    Enlarge

  35. PTPN11

    Plasmid
    #38965
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPN11 (PTPN11 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  37. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid
    #212122
    Purpose
    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.
    Depositor
    Ryan Mehl
    Inserts
    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    Tags
    His6 and SUMO
    Expression
    Bacterial
    Promoter
    araC and lpp
    Available Since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    212122-plasmid-map-sequence-id-444187

    Enlarge

  38. Hs.KRAS4b Q61R

    Plasmid
    #83135
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway entry clone
    Mutation
    Q61R
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83135-plasmid-map-sequence-id-158098

    Enlarge

  39. MECR

    Plasmid
    #38850
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MECR (Mecr Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 7001 - 7040 of 9474 results