We narrowed to 4,380 results for: 1-Oct;

1. pCis_7+8+10

   Plasmid

   #60588

   Purpose

   a 3-integrase plasmid

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Cis_7+8+10
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. GB4653

   Plasmid

   #215267

   Purpose

   TU for the constitutive expression of the flippase (FLP) recombinase with a nopaline synthase (Nos) promoter

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   pNos:FLP:tNos
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Jan. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. GB4650

   Plasmid

   #215265

   Purpose

   TU for the copper regulated expression of the PhiC31 recombinase.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   CBS:miniDFR:PhiC31:tNos
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. GB4832

   Plasmid

   #215276

   Purpose

   Module for the constitutive expression of HISPS, H3H, EGFP and p23.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   HISPS-H3H-EGFP-p23
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. GB4652

   Plasmid

   #215266

   Purpose

   TU for the copper regulated expression of the flippase (FLP) recombinase.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   CBS:miniDFR:FLP:TNOS
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. GB4666

   Plasmid

   #215271

   Purpose

   pUPD2 for the Arabidopsis thaliana ABA-responsive MAPKKK18 promoter.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   promMAPKKK18
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. GB1203

   Plasmid

   #215369

   Purpose

   TU for the expression of the silencing suppressor P19 driven by the 35s promoter

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   35s:P19:tNos
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLT237

   Plasmid

   #174300

   Purpose

   Replicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   PolC homology arm
   

   Expression

   Bacterial

   Mutation

   changed Cysteine 669 to Tyrosine

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDGO186N-KS2

   Plasmid

   #174302

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Expression

   Bacterial

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGO186N-KS3

    Plasmid

    #174303

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pB-puro-mU6-RtcAshRNA1

    Plasmid

    #126613

    Purpose

    Expresses shRNA against human RtcA under mouse U6 promoter

    Depositor

    Xiaozhong Wang

    Article

    Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

    Insert

    RtcA shRNA-1
    

    Use

    RNAi

    Promoter

    mouse U6 promoter

    Available Since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFL-SV40-PACT 5'-UTR

    Plasmid

    #115351

    Purpose

    firefly luciferase (Fluc) reporter containing the 5’-UTR of PACT

    Depositor

    Ming-Chih Lai

    Article

    Lai et al FEBS J. 2016 Jan;283(1):88-101. doi: 10.

    Insert

    PACT 5'-UTR (PRKRA Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUW-SOKM

    Plasmid

    #20325

    Purpose

    Lentiviral plasmid expressing mouse Sox2, Oct4, Klf4 and cMyc for iPS cell generation

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Sox2-P2A-Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastbac-Flag-BRCA1

    Plasmid

    #223228

    Purpose

    Insect expression construct for Flag-tagged BRCA1 protein production and purification

    Depositor

    Weixing Zhao

    Article

    Wang et al Mol Cell. 2023 Oct 19;83(20):3679-3691.e

    Insert

    Breast Cancer 1 (BRCA1 Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-Skp2

    Plasmid

    #89142

    Purpose

    mammalian expression of human Skp2 with N-terminal FLAG tag

    Depositor

    Elizabeth Wilson

    Article

    Su et al Mol Cell Endocrinol. 2017 Jan 5;439:1-9.

    Insert

    Skp2 (SKP2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. nLuc-ΔECD-PTHR1

    Plasmid

    #190011

    Purpose

    Expresses the Nanoluciferase fused to the human parathyroid hormone receptor type 1 that is missing the extracellular domain

    Depositor

    Sam Gellman

    Article

    Yu et al ACS Chem Biol. 2022 Nov 18;17(11):3148-3

    Insert

    nLuc-ΔECD-PTHR1 (PTH1R Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-Ufm1 WT

    Plasmid

    #133865

    Purpose

    Expression of human wild type UFM1

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    Ubiquitin-fold modifier 1 (UFM1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Flag-Jarid2-polyA

    Plasmid

    #223811

    Purpose

    IVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter.

    Depositor

    Azusa Inoue

    Article

    Matsuwaka et al Nat Cell Biol. 2025 Jan;27(1):28-38. doi

    Insert

    Jarid2 (Jarid2 Mouse)
    

    Use

    In vitro transcription

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pACYC-T7

    Plasmid

    #41187

    Depositor

    Dan Bolon

    Article

    Wayne et al J Biol Chem. 2007 Nov 30;282(48):35386-9

    Insert

    S. cerevisiae Hsp82
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET11a-dH2A

    Plasmid

    #105500

    Purpose

    Expression of Drosophila histone H2A

    Depositor

    James Kadonaga

    Article

    Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

    Insert

    dH2A
    

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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