We narrowed to 5,803 results for: SUP

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pLKO.1P shABCB7_2

Plasmid

#102969

Purpose

Suppression of ABCB7

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shABCB7_2 (ABCB7 Human)

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shABCB7_1

Plasmid

#102968

Purpose

Suppression of ABCB7

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shABCB7_1 (ABCB7 Human)

Use

Lentiviral and RNAi

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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spa-GCaMP6s

Plasmid

#67556

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6s

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
BAS292 C terminal SF-GFP kanR

Plasmid

#74087

Purpose

S.pombe recoded super-folder GFP in universal C-terminal tagging plasmid, G418 resistance

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

SF-GFP recoded for S.pombe

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159672

Purpose

Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

βTub-NLS-hSpCas9-T2A-GFP

Tags

T2A-GFP

Expression

Insect

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRJPaph-mTq2

Plasmid

#67031

Purpose

Plasmid for Paph-mTurqoise2 integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
mTurqoise2

Use

Conjugative bacterial vector

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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HA-VHL L188V-pBabe-puro

Plasmid

#19241

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Leucin 188 to Valin

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP[D134TAG+N150TAA]

Plasmid

#164582

Purpose

superfolder GFP with D134TAG and N150TAA mutations

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Insert

sfGFP-134TAG+150TAA

Tags

6xHis

Expression

Bacterial

Mutation

D134TAG + N150TAA Mutations

Promoter

T7

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y98H-pBabe-puro

Plasmid

#19235

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tyrosin 98 to Histidin

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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HA-VHL 1-115-pBabe-puro

Plasmid

#19243

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

deleted amino acids 116 - 213 (end)

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pEN-R2-mCsfGFP-L3

Plasmid

#178602

Purpose

It can be used to study protein turnover in living plant cells of Arabidopsis (Arabidopsis thaliana) and Nicotiana benthamiana.

Depositor

Frederica Theodoulou

Article

Zhang et al Plant Physiol. 2019 Jun;180(2):718-731.

Insert

Tandem fluorescent protein timers (tFTs)

Use

Entry vector for gateway cloning

Tags

mCherry superfolder GFP

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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ninaE-dsocs

Plasmid

#26857

Depositor

Craig Montell

Article

Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

Insert

Slow Termination of Phototransduction (stops Fly)

Tags

myc

Expression

Insect

Mutation

This STOPS construct contains a mutated/truncated…

Available Since

April 5, 2011

Availability

Academic Institutions and Nonprofits only
pSK260

Plasmid

#129252

Purpose

dual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporter

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35 NM yTRAP sensor reporting on mKate2

Expression

Yeast

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBEG R2-iCreT2ALuc-L3 #BGV208

Plasmid

#48992

Purpose

Supplies Cre recombinase and Luciferase and IRES with flanked by AttR2 and AttL3 sites for Gateway cloning recombination reaction. (This corresponds to a module 3 plasmid).

Depositor

David Dankort

Article

Geiling et al PLoS One. 2013 Oct 11;8(10):e76279. doi:

Inserts

cre recombinase
Luciferase

Use

Luciferase; Gateway cloning vector

Tags

IRES (weak)

Promoter

Unknown

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only
pSR-siKlf5

Plasmid

#41740

Depositor

Jonathan Katz

Article

Yang et al FASEB J. 2007 Feb;21(2):543-50. Epub 200

Insert

Klf5 (Klf5 Mouse)

Use

RNAi

Available Since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only
pSK247

Plasmid

#131148

Purpose

dual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreen

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Expression

Yeast

Promoter

pSUP35, min pCYC1

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pIBA3-SpyCatcherEQ-sfGFP

Plasmid

#107421

Purpose

For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

Depositor

Jack Leo

Article

Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

Insert

SpyCatcher

Tags

His tag, StrepII, and superfolder GFP

Expression

Bacterial

Mutation

mutation of glutamate 77 to glutamine

Promoter

Tet

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-SpyTag003 Loop A

Plasmid

#171776

Purpose

Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-SpyTag003 Loop A

Tags

His6

Expression

Bacterial

Mutation

SpyTag003 and linkers inserted between residues V…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159671

Purpose

Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

ExuL-NLS-hSpCas9-T2A-GFP

Tags

T2A-GFP

Expression

Insect

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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sspa-GCaMP6f

Plasmid

#67561

Purpose

short photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

sspa-GCaMP6f

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
spa-GCaMP6f

Plasmid

#67558

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

CMV

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pSR353

Plasmid

#125078

Purpose

Expression of narL under PLtetO-1, output promoter PdcuS77

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

narL (narL )
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pRJPaph-HcRed

Plasmid

#67035

Purpose

Plasmid for Paph-HcRed integration downstream of B. diazoefficiens (B. japonicum) scoI

Depositor

Julia Vorholt

Article

Ledermann et al Mol Plant Microbe Interact. 2015 Jun 2.

Inserts

B. diazoefficiens DNA from scoI downstream region (comprising blr1132')
HcRed

Use

Conjugative bacterial vector

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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HA-VHL T157I-pBabe-puro

Plasmid

#19240

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Threonin 157 to Isoleucin

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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KHBD00340

Plasmid

#34463

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8573 (su(Hw) Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
HA-VHL A149T-pBabe-puro

Plasmid

#19242

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Alanin 149 to Threonin

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-239

Plasmid

#74609

Purpose

Expresses Hook domain and first coiled coil of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-239 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET-spa-GCaMP6f

Plasmid

#67563

Purpose

bacterial expression of a photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6f

Tags

HIS-tag

Expression

Bacterial

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
IRS1-B1

Plasmid

#25698

Depositor

Eric Fearon

Article

Bommer et al J Biol Chem. 2010 Jan 15. 285(3):1928-38

Insert

IRS1 (IRS1 Human)

Use

RNAi and Retroviral

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
MSCV T274V KSR1 IRES GFP

Plasmid

#25977

Depositor

Rob Lewis

Article

Razidlo et al J Biol Chem. 2004 Nov 12. 279(46):47808-

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

changed threonine 274 to valine

Available Since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only