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We narrowed to 5,640 results for: pcas

Showing: 5281 - 5310 of 5640 results

  8. VIPR2-DuET

    Plasmid
    #213387
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    VIPR2 (VIPR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213387-plasmid-map-sequence-id-430740

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  13. pORANGE_NFL linker-3xFLAG KI

    Plasmid
    #182679
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182679-plasmid-map-sequence-id-363017

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  14. pORANGE_NFL linker(A6TAG)-3xFLAG KI

    Plasmid
    #182680
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182680-plasmid-map-sequence-id-363164

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  25. pLCHKO_HPRT1_exon3_1_As

    Plasmid
    #155057
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 3
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155057-plasmid-map-sequence-id-308520

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  26. pLCHKO_HPRT1_exon3_2_As

    Plasmid
    #155058
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 3
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155058-plasmid-map-sequence-id-308441

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  27. pLCHKO_HPRT1_exon2_2_As

    Plasmid
    #155056
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155056-plasmid-map-sequence-id-308440

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  28. pLCHKO_HPRT1_exon2_1_As

    Plasmid
    #155055
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155055-plasmid-map-sequence-id-308438

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  29. pLCHKO_HPRT1_exon2_2_Lb

    Plasmid
    #155052
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155052-plasmid-map-sequence-id-308434

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Showing: 5281 - 5310 of 5640 results