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We narrowed to 12,455 results for: cel.2;

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Showing: 7041 - 7080 of 12455 results

  1. pcDNA4/TO-ORF66 C244A-2xStrep

    Plasmid
    #131121
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C244A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 244 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131121-plasmid-map-sequence-id-257526

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  2. pcDNA4/TO-ORF66 C427A-2xStrep

    Plasmid
    #131109
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C427A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 427 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131109-plasmid-map-sequence-id-257472

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  3. pcDNA4/TO-ORF66 C424A-2xStrep

    Plasmid
    #131110
    Purpose
    Expresses KSHV ORF66 a.a. 1-429 (full length) C424A mutant with a C-terminal 2xStrep tag in mammalian cells
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.
    Insert
    ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    ORF66 a.a. 1-429 (full-length); cysteine 424 muta…
    Promoter
    CMV
    Available Since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    131110-plasmid-map-sequence-id-257473

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  9. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Article
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Human, Synthetic)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CBh
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

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  10. pMini-CMV-NLS-dead R-IscB_ADAR2dd-NES_T2A_mCherry (W98X)_U6-ωRNA

    Plasmid
    #246432
    Purpose
    All-in-one plasmid. Expresses R-IscB_ADAR2dd in mammalian cells for A-to-I RNA editing. Inactive mCherry included to assess A-to-I editing. Spacer included can direct mCherry fluorescence recovery.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID, fused to human ADAR2 deaminase domain
    mCherry
    ωRNA
    Tags
    HIV NES, SGGSSGGSSGSETPGTSESATPESSGGSSGGS linker …
    Expression
    Mammalian
    Mutation
    R-IscB: changed Aspartic Acid 60 to Alanine, chan…
    Promoter
    CMV and U6
    Available Since
    Nov. 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    246432-plasmid-map-sequence-id-490908

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  11. pBig1a zz TEV YBBR POT1 MBP TEV TPP1 MBP TEV TIN2 ZZ TEV TRF2 (4comp2)

    Plasmid
    #185448
    Purpose
    Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF2 with a zz tag and TEV site, and human TIN2 and TPP1 each with an MBP affinity tag and TEV site in insect cells
    Depositor
    Ahmet Yildiz
    Article
    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.
    Inserts
    TRF2 (TERF2 Human)
    POT1 (POT1 Human)
    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    Tags
    MBP, YBBR, and ZZ
    Expression
    Insect
    Mutation
    See Depositor Comments Below
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    185448-plasmid-map-sequence-id-364976

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  12. pCS2-NES-Myc(340-439)-GFP-IRES-mCherry (NES-DBD)

    Plasmid
    #231237
    Purpose
    MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NES for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Expression
    Mammalian
    Mutation
    NES fused to the DNA-binding-domain of MYC (resid…
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231237-plasmid-map-sequence-id-459172

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  13. pCS2-MYC(Δ1-143; Δ340-439)-GFP-IRES-mCherry (ΔTADΔDBD)

    Plasmid
    #231235
    Purpose
    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and DNA-binding-domain (residues 340-439) for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Expression
    Mammalian
    Mutation
    TAD deleted (delta1-143), DBD deleted (delta340-4…
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231235-plasmid-map-sequence-id-459171

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  14. pCS2-NLS-Myc(340-439)-GFP-IRES-mCherry (NLS-DBD)

    Plasmid
    #231236
    Purpose
    MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NLS for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Expression
    Mammalian
    Mutation
    NLS fused to the DNA-binding-domain of MYC (resid…
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231236-plasmid-map-sequence-id-459169

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  16. LPAR2-DuET

    Plasmid
    #213330
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    LPAR2 (LPAR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213330-plasmid-map-sequence-id-430864

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  17. LTB4R2B-DuET

    Plasmid
    #213334
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    LTB4R2B (LTB4R2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213334-plasmid-map-sequence-id-431823

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  20. GPRC5B-DuET

    Plasmid
    #213296
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPRC5B (GPRC5B Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213296-plasmid-map-sequence-id-430840

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  21. pBrain-GFP-TACC3KDP(L566,567A)-shTACC3

    Plasmid
    #69117
    Purpose
    Dual-promoter plasmid to express knockdown-proof human TACC3 (L566A and L567A substitutions) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    LL (566-567aa) mutated to AA. Silent mutations i…
    Promoter
    CMV
    Available Since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    69117-plasmid-map-sequence-id-122092

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  23. pBrain-GFP-TACC3KDP∆678-681-shTACC3

    Plasmid
    #69115
    Purpose
    Dual-promoter plasmid to express knockdown-proof human TACC3 (amino acids 678-681 deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.
    Depositor
    Stephen Royle
    Article
    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:
    Inserts
    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    Use
    RNAi
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Deletion of 678-681aa region. Silent mutations i…
    Promoter
    CMV
    Available Since
    Oct. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    69115-plasmid-map-sequence-id-122094

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  24. CYSLTR2-DuET

    Plasmid
    #213226
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CYSLTR2 (CYSLTR2 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213226-plasmid-map-sequence-id-430788

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  26. pOPINN-GFP

    Plasmid
    #53541
    Purpose
    pTirex based GFP fusion vector for expression in mammalian, E. coli and insect cells using the baculovirus system
    Depositor
    Ray Owens
    Insert
    GFP
    Tags
    N-His-GFP
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53541-plasmid-map-sequence-id-83419

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  27. pcDNA3-His-SUMO2

    Plasmid
    #159324
    Purpose
    Mammalian cell expression of mouse His-SUMO2
    Depositor
    Jianjun Zhao
    Insert
    His-SUMO2 (Sumo2 Mouse)
    Expression
    Mammalian
    Available Since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    159324-plasmid-map-sequence-id-311873

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  33. pD649-HAsp-IL1R2-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156769
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    IL1R2 (IL1R2 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    156769-plasmid-map-sequence-id-382023

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Showing: 7041 - 7080 of 12455 results