-
Plasmid#60151PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB682
Plasmid#60143PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.DepositorInsertFLuc-yEVenus-PEST
UseTagsPEST degronExpressionYeastMutationA4VPromoterMET17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB693
Plasmid#60145PurposeDisintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertYeLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640a-1
Plasmid#42092DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 820-842 nt segment of ZEB1 mRNA (NM_…PromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640s-3
Plasmid#42091DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 4565-4587 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-302s-1
Plasmid#42087DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 4146-4168 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceApril 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-215a-1
Plasmid#42085DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 1259-1281 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640s-2
Plasmid#42090DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 1956-1981 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640s-1
Plasmid#42089DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 1419-1441 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-302a-1
Plasmid#42088DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 4055-4078 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-640a-2
Plasmid#42093DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 1635-1657 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-215a-2
Plasmid#42086DepositorInsertZEB1 (ZEB1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains the 2444-2466 nt segment of ZEB1 mRNA (N…PromoterAvailable sinceFeb. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDD2022
Plasmid#101366PurposeName: pCBG68-RBPjkΔB. RBPjk binding site B mutation in pCBG68-LANApi (pDD2002).DepositorInsertRBPjk binding site B mutation in pCBG68-LANApi (pDD2002).
UseLuciferaseTagsExpressionMutationRBPjk binding site B mutation in pCBG68-LANApi (p…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDD2026
Plasmid#101369PurposeName: pCBG68-LANApiΔTATA. LANApi TATA box mutation in pCBG68-LANApi (pDD2002).DepositorInsertLANApi TATA box mutation in pCBG68-LANApi (pDD2002).
UseLuciferaseTagsExpressionMutationLANApi TATA box mutation in pCBG68-LANApi (pDD200…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDD2027
Plasmid#101370PurposeName: pCBR-LANApiΔTATA. LANApi TATA box mutation in pCBR-K14 (pDD2005).DepositorInsertLANApi TATA box mutation in pCBR-K14 (pDD2005).
UseLuciferaseTagsExpressionMutationLANApi TATA box mutation in pCBR-K14 (pDD2005).PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
p5xNF-kb_RE
Plasmid#118050PurposeGBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motifDepositorInsert5 copies of the NF-kb DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4_CRE-CMVmin-luc2
Plasmid#194384PurposeReporter plasmid for cAMP/Calcium assays; firefly luciferase reporter gene driven by 6x clustered CRE binding sites linked to a minimal CMV promoterDepositorInsert6xCRE-CMVmin-luc2
UseTagsExpressionMammalianMutationPromoterCMV minimalAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
proE-cad178-Luc
Plasmid#42081DepositorInsertE-cadherin (CDH1 Human)
UseLuciferaseTagsExpressionMammalianMutationcontains E-cadherin promoter region from -178 to …PromoterE-cadherinAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMEH31 Lamp2b HA
Plasmid#168208PurposeMammalian expression of lysosome-associated membrane protein 2b fused to a C-terminal HA tagDepositorInsertLamp2b (LAMP2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti HRE-Luc pGK Hygro
Plasmid#118706Purposereporter plasmid HIF-responsive promoter (3xHRE) wt- fused to firefly luciferaseDepositorInsertHRE (HIF-responsive promoter (3xHRE)-luc
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only