We narrowed to 4,074 results for: p2a

1. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA

   Plasmid

   #210501

   Purpose

   expresses LAUNCHER component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. mAC-POLR2A donor (Hygro)

   Plasmid

   #124496

   Purpose

   A donor plasmid for tagging the N-terminus of human POLR2A with mAID-mClover

   Depositor

   Masato Kanemaki

   Article

   Nagashima et al J Cell Biol. 2019 Mar 1. pii: jcb.201811

   Insert

   POLR2A homology arms and Hygro-P2A-mAID-mClover (POLR2A Human)
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-Syn-NS1

   Plasmid

   #175276

   Purpose

   AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)
   

   Use

   AAV

   Tags

   NLS-dTomato (P2A cleavage)

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWZ403

   Plasmid

   #208113

   Purpose

   mSwAP-In marker cassette 1 with mScarlet-PuroTK

   Depositor

   Jef Boeke

   Article

   Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

   Insert

   mScarlet-PuroTK
   

   Use

   Tags

   P2A

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Polymerase-unfused click editor - pCMV-T7-PCV2-nCas9 (LM2702)

   Plasmid

   #208961

   Purpose

   Polymerase-unfused click editor comprised of PCV2 fused to nCas9, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV_Nanog HMEJ donor

   Plasmid

   #97321

   Purpose

   HMEJ donor for fusing a p2A-mCherry reporter to mouse Nanog. AAV backbone.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Nanog HMEJ donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-PE SpRY

   Plasmid

   #179082

   Purpose

   All-in-one prime editor piggyBac transposon, SpRY variant

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9 SpRY_H480A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   A61R, L1111R, D1135L, S1136W, G1218K, E1219Q, N13…

   Promoter

   CAG

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57.1-FLAG-SRSF3_Full.Length_Codon.Optimized-Blast

   Plasmid

   #171951

   Purpose

   All-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, full-length SRSF3

   Depositor

   Sujatha Jagannathan

   Article

   Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

   Insert

   serine and arginine rich splicing factor 3 (SRSF3 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon-optimized

   Promoter

   TRE

   Available since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-PEmax NG

   Plasmid

   #187894

   Purpose

   All-in-one prime editor piggyBac transposon with PEmax, NG variant

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   BPSV40 NLS and c-Myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOT_1 - lenti-EFS-LTBR-2A-puro

    Plasmid

    #181970

    Purpose

    Expresses human LTBR linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    LTBR (LTBR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiCas9-Venus

    Plasmid

    #70267

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    Cas9
    Venus Reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS and P2A

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV_Actb HR donor

    Plasmid

    #97317

    Purpose

    HR donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HR donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSYC-176

    Plasmid

    #178961

    Purpose

    Expresses human GFAP fused to 3xFLAG tag in mammalian cells

    Depositor

    Seok-Yong Choi

    Article

    Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1

    Insert

    Glial fibrillary acidic protein (GFAP Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94 Promoter

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMIA3

    Plasmid

    #109399

    Purpose

    Expresses sgRNA cassette (BsmBI cloning site) and EF1-A driven eSpCas9 linked via P2A with mRuby2 and dominant negative mtp53 for enhanced survival of hES after DSB

    Depositor

    Roger Foo

    Article

    Ang et al Cell Rep. 2018 Feb 20;22(8):2190-2205. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    mRuby2 and mtp53dn

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

    Plasmid

    #229839

    Purpose

    A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV_Actb HMEJ donor

    Plasmid

    #97318

    Purpose

    HMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb HMEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV_Actb NHEJ donor

    Plasmid

    #97316

    Purpose

    NHEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb NHEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV_Actb MMEJ donor

    Plasmid

    #97315

    Purpose

    MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

    Depositor

    Hui Yang

    Article

    Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

    Insert

    Actb MMEJ donor
    

    Use

    AAV and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Control click editor - pCMV-T7-deadPCV2-nCas9-EcKlenow (EMK492)

    Plasmid

    #208943

    Purpose

    A control CE1 construct with catalytically attenuated PCV2 HUH, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    dPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    PCV2(Y96F); nSpCas9(H840A); EcKlenow(-exo;D355A/E…

    Promoter

    CMV and T7

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLNCX2-OptoTGFBRs

    Plasmid

    #118965

    Purpose

    Retroviral expression OptoTGFBRs for transient or stable constitutive expression

    Depositor

    Won Do Heo

    Article

    Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

    Insert

    OptoTGFBRs (Tgfb1, Tgfbr2 Mustard Weed, Mouse)
    

    Use

    Retroviral

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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