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pAc5.1B-lambdaN-HA-DmSmaug_584-999-V5His6_C

Plasmid

#146012

Purpose

Insect Expression of DmSmaug_584-999

Depositor

Elisa Izaurralde

Insert

DmSmaug_584-999 (smg Fly)

Expression

Insect

Mutation

5 silent mutations and a deletion of the AA 961-9…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmSmaug-A642H-V5His6_C

Plasmid

#146014

Purpose

Insect Expression of DmSmaug-A642H

Depositor

Elisa Izaurralde

Insert

DmSmaug-A642H (smg Fly)

Expression

Insect

Mutation

10 silent mutations and a deletion of the AA 961-…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmSmaug-K606AR609A-V5His6_C

Plasmid

#146016

Purpose

Insect Expression of DmSmaug-K606AR609A

Depositor

Elisa Izaurralde

Insert

DmSmaug-K606AR609A (smg Fly)

Expression

Insect

Mutation

10 silent mutations and a deletion of the AA 961-…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSumo-His6- Sumo-OsTPL(1-209)

Plasmid

#177855

Purpose

Bacterial Expression of TPL

Depositor

Karsten Melcher

Article

Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

Insert

TPL

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT/6xHis mCherry F30 Broccoli

Plasmid

#169234

Purpose

Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with Broccoli RNA aptamer.

Depositor

Dora Tang

Article

Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

Insert

6xHis mCherry F30-Broccoli

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-T7Max-UTR1-NTerminus8xHis-T500

Plasmid

#178421

Purpose

Empty backbone. T7Max promoter with N-His Tag

Depositor

Kate Adamala

Article

Deich et al J Biol Eng. 2023 Jan 23;17(1):4. doi: 10

Type

Empty backbone

Use

Synthetic Biology

Tags

8xHis

Expression

Bacterial

Promoter

T7Max

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pSumo-His6-SUMO-AtTPR2(1-209)

Plasmid

#177860

Purpose

Bacterial Expression of TPR2

Depositor

Karsten Melcher

Article

Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

Insert

TPR2 (TPR2 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSumo-His6-SUMO-OsTPR2(1-210)

Plasmid

#177859

Purpose

Bacterial Expression of TPR2

Depositor

Karsten Melcher

Article

Ke et al Sci Adv. 2015 Jul 24;1(6):e1500107. doi:

Insert

TPR2

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO SnRK2.3 (19-361)

Plasmid

#177846

Purpose

Bacterial Expression of SnRK2.3

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2.3 (SNRK2.3 Mustard Weed)

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET Duet1 His6 SUMO D14

Plasmid

#177854

Purpose

Bacterial Expression of D14

Depositor

Karsten Melcher

Article

Zhao et al Cell Res. 2013 Mar;23(3):436-9. doi: 10.

Insert

D14

Tags

6xHis-SUMO

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET24a His6 GST PYL2(14-188)

Plasmid

#177842

Purpose

Bacterial Expression of PYL2

Depositor

Karsten Melcher

Article

Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

Insert

PYR1-like 2 (PYL2 Mustard Weed)

Tags

6xHis-GST

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-DSep1-msfGFP

Plasmid

#174494

Purpose

bacterial expression of Drosophila Sep1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Insert

Sep1 (Sep1 Fly)

Tags

His6-TEV and msfGFP

Expression

Bacterial

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgβ-anticalin-His-ybbr

Plasmid

#168040

Purpose

Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Tags

6x Histag, Fgβ peptide, and ybbr tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET15b p97-Strep-His E314Amb

Plasmid

#169021

Purpose

Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97

Tags

His and SBP

Expression

Bacterial

Mutation

E314TAG

Promoter

T7

Available Since

July 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmTral_394-543-V5His6_D

Plasmid

#146134

Purpose

Insect Expression of DmTral_394-543

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_394-543 (tral Fly)

Expression

Insect

Mutation

two silent mutations (nucleotides A198C, A387G) a…

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

Plasmid

#169162

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)

Tags

6His-3xFlag

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

Plasmid

#169163

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-6His-nsp14 (ORF1ab Synthetic, SARS-CoV-2)

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmUPF2-siRNAres-V5His6_E

Plasmid

#146166

Purpose

Insect Expression of DmUPF2-siRNAres

Depositor

Elisa Izaurralde

Insert

DmUPF2-siRNAres (Upf2 Fly)

Expression

Insect

Mutation

3 non silent mutations: L1022P, V1097L and N1213S…

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmSmaug_763-999-V5His6_C

Plasmid

#146013

Purpose

Insect Expression of DmSmaug_763-999

Depositor

Elisa Izaurralde

Insert

DmSmaug_763-999 (smg Fly)

Expression

Insect

Mutation

2 silent mutations and a deletion of the AA 961-9…

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmSmaug_1-762-V5His6_C

Plasmid

#146001

Purpose

Insect Expression of DmSmaug_1-762

Depositor

Elisa Izaurralde

Insert

DmSmaug_1-762 (smg Fly)

Tags

V5-6xHis

Expression

Insect

Mutation

NP_523987.1, 7 silent mutations compared to the s…

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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