We narrowed to 18,334 results for: met

1. pET28a-ybbR-HIS-Xyl-DocI

   Plasmid

   #58711

   Purpose

   Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   Xyl-DocI
   

   Use

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   changed Threonin 120 to Cysteine in Xylanase Doma…

   Promoter

   T7

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SNF-5 pXOOM

   Plasmid

   #48697

   Purpose

   Expressed SNF-5 in Xenopus oocytes or mammalian cells

   Depositor

   Dmitri Boudko

   Article

   Metzler et al J Exp Biol. 2013 Aug 1;216(Pt 15):2843-5

   Insert

   snf-5 (snf-5 Nematode)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mammalian

   Mutation

   K42E (when compared to NP_496735.1)

   Promoter

   T7

   Available Since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Clover-PDHA1-N-10

   Plasmid

   #56304

   Purpose

   Localization: Mitochondria, Excitation: 505, Emission: 515

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   PDHA1 (PDHA1 Human)
   

   Use

   Tags

   Clover

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pMV-HIS3

   Plasmid

   #65333

   Purpose

   standard plasmids for exogenus pathway assembly haboring yeast auxotrophic marker HIS3 which could be released and ligated to series of TUs

   Depositor

   Junbiao Dai

   Article

   Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

   Insert

   HIS3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFAB815

   Plasmid

   #47854

   Purpose

   BIOFAB reporter plasmid for measuring rnpB T1 termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   rnpB T1 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLTetO9

   Available Since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEN-X-hPGK-PuroR-Z

   Plasmid

   #218904

   Purpose

   golden gate cloning

   Depositor

   Sven Eyckerman

   Article

   Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

   Insert

   hPGK-PuroR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   246T>A and 398T>C

   Promoter

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro

   Plasmid

   #31480

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Traffic Light Reporter 1.1 (ani target)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   mCherry has M9S, M16L to reduce background mCherr…

   Promoter

   Available Since

   Aug. 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFU-MVIIA-PE

   Plasmid

   #24144

   Depositor

   Ines Ibanez-Tallon

   Article

   Auer et al Nat Methods. 2010 Mar;7(3):229-36. doi:

   Insert

   omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Conus magus, Aequorea victoria, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 28, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. peCALWY-2

   Plasmid

   #22234

   Depositor

   Maarten Merkx

   Article

   Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

   Insert

   eCALWY-2 Zn(II) sensor (ATOX1 Human, GFP's)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   sequence identical to eCALWY-1, but linkerlength …

   Promoter

   Available Since

   Nov. 20, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pRSFDuet-1_VNp15-DARPinOFF7

    Plasmid

    #182399

    Purpose

    Bacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp15-DARPinOFF7
    

    Use

    Tags

    Vesicle Nucleating peptide 15 (VNp15)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEMT-Map7-1333-end

    Plasmid

    #46070

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-1333-end (Map7 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21-pelB-LaG-17

    Plasmid

    #172747

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-17
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCFJ1208

    Plasmid

    #44490

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1 [Peft-3 tdTomato(NLS) tbb-2 UTR, unc-119]
    

    Use

    Gateway expression clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. eGFP-RAPH1-F1

    Plasmid

    #194860

    Purpose

    Express the RAPH1 fragment (1-535) tagged to eGFP in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

    Insert

    RAPH1 (RAPH1 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    amino acids 1-535

    Promoter

    CMV

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mIFP-Lysosomes-20

    Plasmid

    #56228

    Purpose

    Localization: Lysosome Membrane, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    LAMP1
    

    Use

    Tags

    mIFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. piGECI-NES-N1

    Plasmid

    #160422

    Purpose

    Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI-NES
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mRuby2-Nup50-N-10

    Plasmid

    #55908

    Purpose

    Localization: Nuclear Pore, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    Nup50 (NUP50 Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pMXS-IRES-Blast SHMT1

    Plasmid

    #106304

    Purpose

    Express SHMT1

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    serine hydroxymethyltransferase 1 (SHMT1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-SMVP-Cas9N-U6-gRNA TdL

    Plasmid

    #80938

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Gi-c009

    Plasmid

    #173079

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    GNAI1 (GNAI1 Human)
    

    Use

    Tags

    mCitrine

    Expression

    Bacterial

    Mutation

    full length, codon optimized

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge