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pMYT095

Plasmid

#198866

Purpose

CRISPR/Cas9 Vector with URA3 selectable marker to be used in the Ellis MYT system

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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LeGO-1xT/BSD

Plasmid

#27353

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

U6 promoter, SFFV promoter, dTomato/BSD-Fusion

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pYPQ148

Plasmid

#69300

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 8 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_mVenus

Plasmid

#118861

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Article

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

mVenus

Tags

His-tag

Expression

Bacterial

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->HA-mtC11L34

Plasmid

#162227

Purpose

Lentiviral vector for the expression of HA mtC11L34 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

mtC11L34

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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1x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64390

Purpose

encodes one a1 operator site upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Expression

Yeast

Available Since

May 5, 2015

Availability

Academic Institutions and Nonprofits only

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pLV hMyoD-IRES-dsRed

Plasmid

#66631

Purpose

Co-expresses human MyoD and dsRed

Depositor

Charles Gersbach

Insert

human MyoD IRES dsRed (MYOD1 Human)

Use

Lentiviral

Promoter

hUbc

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pH

Plasmid

#64178

Purpose

harbours marker gene HIS3

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pWS3868

Plasmid

#185745

Purpose

Sub-array backbone 4/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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p70 S6 kinase

Plasmid

#1892

Depositor

Joseph Avruch

Article

Weng et al Mol Cell Biol 1995 May;15(5):2333-40.

Insert

p70 alpha1 S6 kinase (Rps6kb1 Rat)

Tags

HA

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pEntr-Nkx2-5flbio

Plasmid

#32969

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

Nkx2-5 (Nkx2-5 Mouse)

Use

Gateway

Tags

flag-bio

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pM

Plasmid

#64179

Purpose

harbours marker gene MET17

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pED14xS-1

Plasmid

#134800

Purpose

IPTG inducible expression of S-TAGA qtRNA

Depositor

Ahmed Badran

Article

DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

Insert

S-TAGA qtRNA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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mEYFP-(L)-mCherry2-(L)-mEGFP-(L)-mApple

Plasmid

#182862

Purpose

Hetero-tetramer expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

Insert

mEYFP-mCherry2-mEGFP-mApple

Expression

Mammalian

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-sypHTomato

Plasmid

#164613

Purpose

Express the pHTomato inserted in the synaptophysion in mammalian cells

Depositor

Yulong Li, Richard Tsien

Article

Li et al Nat Neurosci. 2012 May 27;15(7):1047-53.

Insert

A reporter of vesicular turnover sypHTomato

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pL

Plasmid

#64177

Purpose

harbours marker gene LEU2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Aug. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pJFRC170-3XUAS-IVS-Cre::PEST

Plasmid

#32144

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

Cre::PEST

Expression

Insect

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pYPQ147

Plasmid

#69299

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 7 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pBNJ24.6

Plasmid

#66873

Purpose

for transposon tagging with a fluorescent protein

Depositor

Thomas Hughes

Article

Sheridan et al BMC Biotechnol. 2004 Aug 18;4:17.

Insert

eGFP in a transposon

Use

Source for a tn7 transposon

Tags

eGFP

Promoter

none

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only
pTU1

Plasmid

#72934

Purpose

Backbone vector with internal BsaI removed by site-directed mutagenesis

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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