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pcDNA5-hygro-eGFP-HEC1-8A/S55D

Plasmid

#114032

Purpose

Inducible expression of eGFP-tagged HEC1-8A/S55D in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1-8A/S55D (NDC80) (NDC80 Human)

Expression

Mammalian

Mutation

Hec1 - 8A/S55D

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag Jnk3a2(2nd methione)

Plasmid

#15747

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk3a2(2nd methione) (MAPK10 Human)

Expression

Bacterial

Available Since

March 6, 2008

Availability

Academic Institutions and Nonprofits only
DVA_DH

Plasmid

#66049

Purpose

MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [D:LacZa:H]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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DVA_EB

Plasmid

#66050

Purpose

MoClo Destination Vector: (based on pSB1A2 with fixed illegal site) [E:LacZa:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Type

Empty backbone

Use

Synthetic Biology; Destination vector

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-160

Plasmid

#74607

Purpose

Expresses Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2473

Plasmid

#36854

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CCND1-TALEN_Right (Ccnd1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2304

Plasmid

#36733

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CCND1-TALEN_Left (Ccnd1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2386

Plasmid

#36795

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CYLD-TALEN_Left (Cyld Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pFastBac Dual PNG-FLAG PLU-HIS

Plasmid

#113173

Purpose

Baculovirus expression of PNG and PLU

Depositor

Terry Orr-Weaver

Article

Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

Insert

PNG, PLU

Use

Baculovirus

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pTAtUbq3 (GB0219)

Plasmid

#68192

Purpose

Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtUbq3 terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag BimL (S44D)

Plasmid

#24235

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44D) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed serine 44 to aspartic acid

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pGST1-GGA1-VHS

Plasmid

#44418

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

GGA1-VHS (1-148) (GGA1 Human)

Tags

GST

Expression

Bacterial

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157)

Plasmid

#86923

Purpose

To express Ubl4A (90-157 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 1-89 are deleted

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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TAL2320

Plasmid

#36746

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FLT4-TALEN_Left (Flt4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2321

Plasmid

#36747

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FLT4-TALEN_Right (Flt4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
CCD+BATS

Plasmid

#30499

Depositor

Qing Zhong

Article

Fan et al Proc Natl Acad Sci U S A. 2011 May 10;10

Insert

Barkor (ATG14 Human)

Tags

3xFlag and hrGFP

Expression

Mammalian

Mutation

71-180aa (CCD) + 413-492aa (BATS)

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
pET15b+PCNA3 R112C/T180C

Plasmid

#66182

Purpose

Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Mutation

R112C, T180C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pET15b+PCNA2 E105C/L171C

Plasmid

#66178

Purpose

Expresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Mutation

E105C, L171C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pKG427

Plasmid

#69754

Purpose

Retroviral expression of DSN1

Depositor

Iain Cheeseman

Article

Gascoigne et al J Cell Biol. 2013 Apr 1;201(1):23-32. do

Insert

DSN1 (DSN1 Human)

Use

Retroviral

Tags

eGFP-TEV-S

Expression

Mammalian

Mutation

all K to R mutations

Available Since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only
TAL2387

Plasmid

#36796

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CYLD-TALEN_Right (Cyld Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2206

Plasmid

#36669

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FLCN-TALEN_Left (Flcn Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2207

Plasmid

#36670

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FLCN-TALEN_Right (Flcn Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2270

Plasmid

#36707

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

GLI1-TALEN_Left (GLI1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag BimL (S44A)

Plasmid

#24234

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44A) (BCL2L11 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed Serine 44 to Alanine

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
5mCUC1 pBluescript

Plasmid

#12065

Depositor

David Bartel

Article

Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Insert

CUC1 mutant (CUC1 Mustard Weed)

Expression

Bacterial

Mutation

miR-resistant CUC1. Made by introducing five sile…

Available Since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (T56D)

Plasmid

#24532

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(T56D) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed threonine 56 to aspartic acid

Available Since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
FRIG-myc-Sema4DdeltaC

Plasmid

#51607

Purpose

expresses myc-tagged Sema4D lacking C terminus in lentiviral backbone

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4DdeltaC (Sema4d Mouse)

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Mutation

deleted last 70 amino acids in the C-terminus

Promoter

RSV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
pCINTBeta1

Plasmid

#14067

Depositor

Richard Hynes

Article

Tamkun et al Cell. 1986 Jul 18. 46(2):271-82.

Insert

Chicken Beta 1 Integrin (ITGB1 Chicken)

Expression

Mammalian

Mutation

PstI-BglI fragment was blunt end cloned and EcoRI…

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pGST1-GGA2-VHS

Plasmid

#44419

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

GGA2-VHS (GGA2 Human)

Tags

GST

Expression

Bacterial

Mutation

Alanine 23 changed to Threonine

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
TAL2324

Plasmid

#36750

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MAP2K4-TALEN_Left (Map2k4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2325

Plasmid

#36751

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MAP2K4-TALEN_Right (Map2k4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2448

Plasmid

#36837

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PDGFRB-TALEN_Left (Pdgfrb Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2449

Plasmid

#36838

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PDGFRB-TALEN_Right (Pdgfrb Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (S58A)

Plasmid

#24249

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(S58A) (BCL2L11 Human)

Tags

T7

Expression

Mammalian

Mutation

Changed serine 58 to alanine

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
p072 pCS xGATA4 + xTRPS1(C119)

Plasmid

#11043

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

GATA4 + TRPS1(C119) (trps1, gata4-b Frog)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Fusion construct of XGATA4 (amino acids 1-352) wi…

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
DJ-L1 gRNA #5

Plasmid

#247545

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
DJ-L1 gRNA #4

Plasmid

#247544

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
DJ-L1 gRNA #3

Plasmid

#247543

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
DJ-L1 gRNA #1

Plasmid

#247542

Purpose

CRISPRi-KD of human DJ LINE1

Depositor

Miguel Ramalho-Santos

Article

Ataei et al Genes Dev. 2025 Feb 3;39(3-4):280-298. d

Insert

sgRNA targeting human L1DJ

Use

CRISPR

Expression

Mammalian

Mutation

none

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Syntaxin1A(E53TAG)-mCherry

Plasmid

#241293

Purpose

Mammalian expression of syntaxin-1(E53TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. 2025 Nov 24:S0969-2126(25)003

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E53TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Syntaxin1A(E206TAG)-mCherry

Plasmid

#241295

Purpose

Mammalian expression of syntaxin-1(E206TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. 2025 Nov 24:S0969-2126(25)003

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E206TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Syntaxin1A(K84TAG)-mCherry

Plasmid

#241297

Purpose

Mammalian expression of syntaxin-1(K84TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. 2025 Nov 24:S0969-2126(25)003

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

K84TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Syntaxin1A(E49TAG)-mCherry

Plasmid

#241291

Purpose

Mammalian expression of syntaxin-1(E49TAG) fused to mCherry for genetic code expansion via Amber codon supression

Depositor

Justin Taraska

Article

Obashi et al Structure. 2025 Nov 24:S0969-2126(25)003

Insert

Syntaxin-1A (Stx1a Rat)

Tags

mCherry

Expression

Mammalian

Mutation

E49TAG

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_DualsgRNA_TIMM23_Puro_T2A_BFP2

Plasmid

#236731

Purpose

Dual sgRNA targeting TIMM23 expressing BFP with Puromycin selection

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TIMM23 (TIMM23 Human)

Use

Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_DualsgRNA_TIMM23_ER-dAPEX-BFP2

Plasmid

#236734

Purpose

Dual sgRNA targeting TIMM23 expressing dAPEX-BFP targeting to ER

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

TIMM23 (TIMM23 Human)

Use

CRISPR and Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-Neo1 gRNA

Plasmid

#246575

Purpose

CRISPR vector co-expressing Cas9 and a mouse Neo1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Neo1 gRNA (Neo1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-Nrp1 gRNA

Plasmid

#246578

Purpose

CRISPR vector co-expressing Cas9 and a mouse Nrp1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Nrp1 gRNA (Nrp1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-Plxna1 gRNA

Plasmid

#246574

Purpose

CRISPR vector co-expressing Cas9 and a mouse Plxna1 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Plxna1 gRNA (Plxna1 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
sh-hCHIP

Plasmid

#246751

Purpose

Mammalian and lentiviral expression of shRNA targeting human CHIP (Stub1)

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Insert

sh-hCHIP (STUB1 Human)

Use

Lentiviral and RNAi

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His-Thrombin-Munc13-1 C1-C2B-MUN-C2C (aa529-1735 delta1408-1452 delta1533-1551)

Plasmid

#243821

Purpose

Munc13-1 protein purification

Depositor

Jose Rizo-Rey

Article

Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

Insert

Munc13-1 (Unc13a Rat)

Tags

His

Expression

Bacterial

Mutation

delta1408-1452 delta1533-1551

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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