We narrowed to 26,320 results for: sta

1. pENTR1A DHFR.cHSF1

   Plasmid

   #134737

   Purpose

   Gateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domain

   Depositor

   Matthew D. Shoulders

   Article

   Phillips et al Elife. 2017 Sep 26;6. doi: 10.7554/eLife

   Insert

   DHFR.cHSF1 (HSF1 Human)
   

   Use

   Entry vector for gateway cloning

   Tags

   DHFR

   Expression

   Mutation

   Deletion of amino acids 186-202

   Promoter

   None

   Available since

   Oct. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNAintron-CHIKV-nsp1-c3xFLAG

   Plasmid

   #215694

   Purpose

   Produces the Chikungunya virus nonstructural protein 1 with a c-terminal 3xFLAG tag

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV nsp1
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pcDNAintron-CHIKV-nsp2-c3xFLAG

   Plasmid

   #215695

   Purpose

   Produces the Chikungunya virus nonstructural protein 2 with a c-terminal 3xFLAG tag

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV nsp2
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pcDNAintron-CHIKV-nsp4-c3xFLAG

   Plasmid

   #215697

   Purpose

   Produces the Chikungunya virus nonstructural protein 4 with a c-terminal 3xFLAG tag

   Depositor

   Melinda Brindley

   Article

   Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

   Insert

   CHIKV nsp4
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA3-Ha-Brca1 S1387A

   Plasmid

   #32532

   Depositor

   Michael Kastan

   Article

   Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

   Insert

   Brca1 (BRCA1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S1387A

   Promoter

   CMV/Rabbit B-Globin

   Available since

   Oct. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE

   Plasmid

   #123360

   Purpose

   Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Methods. 2019 May;16(5):405-408. doi

   Insert

   Empty LbCpf1 crRNA cloning cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSP2253

   Plasmid

   #70704

   Purpose

   Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Inserts

   mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
   SaCas9 sgRNA (+84)
   

   Use

   CRISPR

   Tags

   NLS-3xFLAG

   Expression

   Bacterial

   Mutation

   E782K, N968K and R1015H in SaCas9

   Promoter

   T7

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Ha-Brca1 S1423A/S1524A

   Plasmid

   #32539

   Depositor

   Michael Kastan

   Article

   Xu et al Cancer Res. 2002 Aug 15;62(16):4588-91.

   Insert

   BRCA1 (BRCA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S1423A/S1524A

   Promoter

   CMV/rabbit B-Globin

   Available since

   Oct. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

9. PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA

   Plasmid

   #172226

   Purpose

   Expression vector with Shield-1 stabilizable hSOX17 fused to TagBFP.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Piggybac transposon

   Tags

   3XFLAG, DD-domain, GS-linker, and TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. DDX5 D248N V5

    Plasmid

    #199577

    Purpose

    Expresses helicase dead DDX5 cloned into pLenti puro

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    DDX5 (DDX5 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    D248N

    Promoter

    CMV

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. UBQLN2_450-624W

    Plasmid

    #176852

    Purpose

    Expresses human UBQLN2 construct 450-624 with added C-terminal W in bacterial cells

    Depositor

    Carlos Castañeda

    Article

    Dao et al Mol Cell. 2018 Mar 15;69(6):965-978.e6.

    Insert

    UBQLN2_450C_W (UBQLN2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    deletion of residues 1-449 and addition of C-term…

    Promoter

    T7

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

    Plasmid

    #156441

    Purpose

    For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SA2 (Stag2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PDF1::mCitrine-MBD

    Plasmid

    #115222

    Purpose

    Microtubule visualization

    Depositor

    Jan Traas

    Article

    Armezzani et al Development. 2018 Jun 4;145(11). pii: de

    Insert

    Microtubule binding domain
    

    Use

    Tags

    mCitrine

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJL1-mRFP1 gRNA

    Plasmid

    #117052

    Purpose

    In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    anti-mRFP1 SpCas9 gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDIRECT_26G

    Plasmid

    #91149

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. HOXB2 (human) HIS-tag pET

    Plasmid

    #8522

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB2 (HOXB2 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Starts at AA #2; makes protein by Western

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pEGFP-C1-KSENB-Nup133-mut

    Plasmid

    #87339

    Purpose

    To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes; deletion of Nup133 AA1-7

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_SNORD27h

    Plasmid

    #73066

    Purpose

    expression clone for human SNORD27 (C/D box snoRNA U27)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD27 (SNORD27 Human)
    

    Use

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    expression cassette for SNORD27

    Promoter

    CMV

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4/TO-BcRF1-2xStrep

    Plasmid

    #138436

    Purpose

    Expresses full-length BcRF1 with a C-terminal 2xStrep tag in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    BcRF1 (BcRF1 EBV (HHV-4))
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. Clim-DC2-NMM-tdtomato

    Plasmid

    #129557

    Purpose

    pClimDC-NMM:tdT cell membrane marker

    Depositor

    Inaki Ruiz-Trillo

    Article

    Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

    Insert

    N-Myristoylation motif (NMM) fused to tdtomato
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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