-
Plasmid#215009PurposeExpress a Lysophagy reporter in mammalian cells (untested)DepositorInsertLGALS3 (LGALS3 Human)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-tdMCP2-GyG-(GGS)X6-HaloTag C261
Plasmid#171434PurposeExpression of tdMCP2-GyG-(GGS)X6-HaloTag C261 in mammalian cellsDepositorInserttdMCP2-GyG-(GGS)X6-HaloTag C261
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha-HaloTag TMD Cys-Ala
Plasmid#179716PurposeLentiviral expression of mouse Syt7 palmitoylation site mutant with C terminal HaloTagDepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)
Plasmid#153005PurposeExpresses high-affinity split-TurboID C-terminal fragment in mammalian cellsDepositorInsertsTurboID-high affinity (C) (G79)
UseLentiviralTagsFRB, HA, and HaloTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP)
Plasmid#215015PurposeExpress an autophagy (LC3) reporter in mammalian cells (untested)DepositorInsertMAP1LC3B (Map1lc3b Rat)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)
Plasmid#215010PurposeExpress a Mitophagy reporter in mammalian cells (untested)DepositorInsertpSu9 (Presequence of Fo-ATPase subunit 9)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-HaloTag-GB1-NLS-dWPRE
Plasmid#106303PurposeThe plasmid encodes the scFv against GCN4 peptide fused with HaloTag and a NLS for SunTag imagingDepositorInsertscFv-GCN4-HaloTag-GB1-NLS
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME Actin Vhh Halo Tag (JDW 1223)
Plasmid#224498PurposeGateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)DepositorInsertActin-Vhh-Halo-Tag
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf
Plasmid#175524PurposeCo-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cellsDepositorInsertBeta4Gal-HaloTag7 and Lyn11-SNAPf
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)
Plasmid#215002PurposeShuttling vector for an ERphagy reporterDepositorInsertKDEL
UseGatewayTagsHaloTag(7)-mGFPExpressionMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)
Plasmid#215014PurposeExpress an autophagy (LC3) reporter in mammalian cells (untested)DepositorInsertMAP1LC3B (Map1lc3b Rat)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)
Plasmid#215011PurposeExpress a Mitophagy reporter in mammalian cells (untested)DepositorInsertpSu9 (Presequence of Fo-ATPase subunit 9)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
R726-M01-892: His6-Halotag-tev-Hs.RAF1(52-131)
Plasmid#159696PurposeE. coli protein expression of His6-Halotag-tev-Hs.RAF1(52-131)DepositorInsertHis6-Halotag-tev-Hs.RAF1(52-131) (RAF1 Human)
UseTagsHis6-Halotag-tevExpressionBacterialMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCA26-pLenti.CAG.-NLS-dCas13b-NLS-VPR-T2A-HaloTag
Plasmid#199457PurposeExpression of dCas13-VPR in mammalian cellsDepositorInsertdCas13
UseLentiviralTagsVPR-T2A-HaloTagExpressionMammalianMutationNuclease-dead H133A and H1058APromoterCAGAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2059 - [1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)
Plasmid#159872PurposeThree-fragment Gateway compatible protein tag for expression in C. elegansDepositorInsert[1-2] ENTR - Tag - Halo (intron(1), syntrons(3), no_atg, no_stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPW3761 : CMVd3-HsIRE1(WLLI-GSGS)[359-362]-HaloTag
Plasmid#185683PurposeLow-level transient expression of IF2 mutant HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Synthetic, Human)
UseTagsHaloTagExpressionMammalianMutation(WLLI-GSGS)[359-362]PromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti UBC ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)
Plasmid#215012PurposeExpress a ERphagy reporter in mammalian cells (untested)DepositorInsertKDEL
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE pGK ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)
Plasmid#215013PurposeExpress a ERphagy reporter in mammalian cells (untested)DepositorInsertKDEL
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r
Plasmid#162956PurposeGateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteinsDepositorInsertHis6-Halotag fusion tag with upstream HTLV RU5 5' UTR
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r
Plasmid#162965PurposeGateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expressionDepositorInsertHis6-Halotag fusion tag with upstream E. coli ribosome binding site
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only