We narrowed to 6,639 results for: WUS

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GFP-Rab9a

Plasmid

#169071

Purpose

Express human Rab9a in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab9a (RAB9A Human)

Tags

acGFP1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a- mYFP-kanMX6

Plasmid

#105147

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mYFP-kanMX6

Tags

mYFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Lari-BFP2-TSERex

Plasmid

#123311

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Lari-BFP2

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M

Plasmid

#22034

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-C450M (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Expression

Mammalian

Mutation

The LOV domain contains the mutation C450M.Rac1 s…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pBabe-TetCMV-puro-mPA-GFP-PA-Rac1

Plasmid

#22032

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mPA-GFP-PA-Rac1 (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
FLAG-mBAF45b IRES GFP

Plasmid

#17881

Depositor

Jerry Crabtree

Article

Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

Insert

BAF45b (Dpf1 Mouse)

Tags

FLAG and IRES EGFP

Expression

Mammalian

Available Since

May 13, 2008

Availability

Academic Institutions and Nonprofits only

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pGEX-NEMOL329P

Plasmid

#11967

Depositor

Jon Ashwell

Article

Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

Insert

NEMOL329P (IKBKG Human)

Tags

GST

Expression

Bacterial

Mutation

Leucine329 to Proline

Available Since

July 13, 2006

Availability

Academic Institutions and Nonprofits only
pCW57-mcGAS

Plasmid

#185458

Purpose

Inducible FLAG-tagged mouse cGAS

Depositor

Shitao Li

Article

Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

Insert

cGAS

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-α-synuclein

Plasmid

#205713

Purpose

Mammalian expression of mNeonGreen-alpha-synuclein

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein (SNCA Human)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pNapin-dsRED

Plasmid

#127798

Purpose

Napin Promoter driven dsRED expression

Depositor

Scott Poethig

Article

Wu et al Genetics. 2015 May;200(1):35-45. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

Plasmid

#22023

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Expression

Mammalian

Mutation

The LOV domain contains the mutation C450A.Rac1 s…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pET28a-PKM2-R399E

Plasmid

#122691

Purpose

Express His-tagged PKM2 (R399E) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Mutation

R399E

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-PKM2-G415R

Plasmid

#122693

Purpose

Express His-tagged PKM2 (G415R) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Mutation

G415R

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac HTB MBP-SARM1 A600G

Plasmid

#195764

Purpose

Purification of SARM1 from insect cells

Depositor

Hao Wu

Insert

SARM1 (SARM1 Human)

Tags

HIS-MBP-TEV

Expression

Insect

Mutation

A600G

Promoter

polyhedrin

Available Since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a- mEGFP-kanMX6

Plasmid

#105146

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mEGFP-kanMX6

Tags

mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCB301TOR-GFP

Plasmid

#164799

Purpose

Expression of GFP in oomycetes using Agrobacterium-mediated transformation

Depositor

Miaoying Tian

Article

Wu et al BMC Microbiol. 2016 Sep 6;16(1):204. doi

Insert

GFP

Use

Oomycete expression

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-OmpT

Plasmid

#68862

Purpose

construct for expression of OmpT protease in the pET28a plasmid using the restriction sites NcoI and EcoRI.

Depositor

Neil Kelleher

Article

Wu et al Nat Methods. 2012 Jun 17;9(8):822-4. doi

Insert

OmpT (ompT )

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pN3-pHluorinCAAX

Plasmid

#123313

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CMV promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

pHluorinCAAX

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-Gncp-iGluSnFR

Plasmid

#107337

Purpose

Noncircularly permutated (ncp) variant of iGluSnFR. Membrane-displayed.

Depositor

Robert Campbell

Article

Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

Insert

GltI based and noncircularly permutated glutamate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-TetCMV-puro-mCerulean-PA-Rac1

Plasmid

#22031

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mCerulean-PA-Rac1 (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Expression

Mammalian

Mutation

Rac1 starts at I4 and contains mutations Q61L, E9…

Available Since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pLenti-GRET

Plasmid

#158222

Purpose

Control lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

GFP-Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EFS-DIO-GRAB_Ado1.0med

Plasmid

#153292

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cells

Depositor

Yulong Li

Article

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Promoter

EFS

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-Rab7Q67L

Plasmid

#169038

Purpose

Express human constitutively active form of Rab7a(Q67L) in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab7a (RAB7A Human)

Tags

acGFP1

Expression

Mammalian

Mutation

Q67L

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCB301TOR

Plasmid

#164798

Purpose

Oomycete expression vector using Agrobacterium-mediated transformation

Depositor

Miaoying Tian

Article

Wu et al BMC Microbiol. 2016 Sep 6;16(1):204. doi

Insert

NPTII

Use

Oomycete expression

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Tom20-mCh-FKBP

Plasmid

#162436

Purpose

for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

Tom20-mCh-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAV-U6+27-Tornado-F30-TAR Variant-1

Plasmid

#129406

Purpose

circular TAR Variant-1

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

circular F30-TAR Variant-1

Expression

Mammalian

Promoter

U6

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a- mCFP-kanMX6

Plasmid

#105145

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

mCFP-kanMX6

Tags

mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO-Bcl2-IRES-tdtomato(TET005)

Plasmid

#117857

Purpose

This plasmid expresses Bcl2 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

Depositor

C. Ron Yu

Article

Wu et al Neuron. 2018 Dec 5;100(5):1066-1082.e6.

Insert

Bcl2 (Bcl2 Mouse)

Use

Tta/teto

Expression

Mammalian

Promoter

tetO

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET29b-rhMOG

Plasmid

#225484

Purpose

Express the soluble domain of Myelin Oligodendrocyte Glycoprotein (rhMOG) in SHuffle E. coli

Depositor

John Pak

Article

Wu et al J Immunol Methods. 2024 Oct 13:113764. d

Insert

Soluble Domain of Myelin Oligodendrocyte Glycoprotein (MOG Human)

Tags

6xHis tag

Expression

Bacterial

Mutation

deleted amino acids 1-29 (signal peptide) and 150…

Promoter

T7

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBOBI TP53 S46D

Plasmid

#213002

Purpose

expresses human P53

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

P53 (TP53 Human)

Expression

Mammalian

Mutation

S46D

Promoter

cmv

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBOBI C-FLAG PHGDH T78A

Plasmid

#212997

Purpose

expresses human PHGDH

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

PHGDH (PHGDH Human)

Tags

FLAG

Expression

Mammalian

Mutation

T78A

Promoter

cmv

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBOBI C-FLAG PHGDH R135W

Plasmid

#212998

Purpose

expresses human PHGDH

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

PHGDH (PHGDH Human)

Tags

FLAG

Expression

Mammalian

Mutation

R135W

Promoter

cmv

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBOBI N-HA AXIN1

Plasmid

#213000

Purpose

expresses mouse AXIN1

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

AXIN1 (Axin1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

cmv

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBOBI C-FLAG PHGDH V261M

Plasmid

#212999

Purpose

expresses human PHGDH

Depositor

Sheng-cai Lin

Article

Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

Insert

PHGDH (PHGDH Human)

Tags

FLAG

Expression

Mammalian

Mutation

V261M

Promoter

cmv

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAC-BETAipi-ispA/dxs

Plasmid

#178302

Purpose

Produces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Inserts

ispA
dxs

Expression

Bacterial

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRV FOXP3 WRR

Plasmid

#13624

Depositor

Anjana Rao

Article

Wu et al Cell. 2006 Jul 28. 126(2):375-87.

Insert

FOXP3 (FOXP3 Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

WRR: N361W, E399R, E401R

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
p23-mNeongreen-DDX21 WT

Plasmid

#179228

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Wu et al Science. 2021 Jul 30;373(6554):547-555.

Insert

DDX21 (DDX21 Human)

Tags

mNeongreen

Expression

Mammalian

Promoter

EF-1alpha

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC119 T7 hTRmin

Plasmid

#127858

Purpose

T7 RNA Pol for in vitro transcription

Depositor

Kathleen Collins

Article

Wu et al EMBO J. 2014 Apr 16;33(8):921-35. doi: 1

Insert

hTR (TERC Human)

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-Orange_CaMBI_110

Plasmid

#124094

Purpose

orange calcium-modulated bioluminescent indicator, 110nM

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Orange CaMBI 110

Expression

Mammalian

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pWay21-MKP-1L16A/L17A

Plasmid

#13478

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L16A/L17A (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 16 to Alanine; changed Leucine 1…

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only

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