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Showing: 681 - 720 of 4899 results

  6. H52-pUCM-hNGN2-inv

    Plasmid
    #110492
    Purpose
    Donor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuron, TALEN cut site outside of homology arm
    Depositor
    Michael Ward
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    mcherry
    rtTA3G
    Use
    CRISPR, Cre/Lox, and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG, EF-1 alpha, and TRE3G
    Available since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    110492-plasmid-map-sequence-id-207872

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  8. pUCmini-iCAP-AAV.CAP-B10

    Plasmid
    #175004
    Purpose
    non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
    Depositor
    Viviana Gradinaru
    Article
    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do
    Insert
    Synthetic construct isolate AAV.CAP-B10 VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    175004-plasmid-map-sequence-id-347131

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  12. pUCmini-iCAP-AAV.CAP-B22

    Plasmid
    #175005
    Purpose
    non-standard AAV2 rep-AAV.CAP-B22 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system
    Depositor
    Viviana Gradinaru
    Article
    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do
    Insert
    Synthetic construct isolate AAV.CAP-B22 VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    175005-plasmid-map-sequence-id-347133

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  19. pUC-dirTopo-P2A-aGFP

    Plasmid
    #61974
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61974-plasmid-map-sequence-id-101282

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  20. pUC-dirTopo-AU1-aGFP

    Plasmid
    #61973
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Promoter
    Available since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61973-plasmid-map-sequence-id-101284

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  22. pUCM-CLYBL-NGN2-BRN3A

    Plasmid
    #165597
    Purpose
    Donor construct for insertion of NGN2 and BRN3A into the CLYBL safe harbor site. This is an all-in-one doxycycline-inducible construct, enabling iPSC differentiation into peripheral sensory neurons.
    Depositor
    Carsten Bönnemann
    Article
    Nickolls et al Cell Rep. 2020 Jan 21;30(3):932-946.e7.
    Inserts
    Neurogenin 2 (NEUROG2 Human)
    BRN3A (POU4F1 Human)
    EGFP
    Puromycin N-acetyl transferase
    rtTA3G
    SBP Delta-LNGFR
    Use
    CRISPR and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG, EF-1alpha, Endogenous CLYBL (splice acceptor…
    Available since
    March 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    165597-plasmid-map-sequence-id-326012

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  35. pVH1002-Tier2(pUC ori)

    Plasmid
    #169600
    Purpose
    Tier-2 expression vector (A1-pA::A2-pA::A3-pA). The tier-2 expression vector consists of a custom MCS cassette containing the three Tier-1 compatible acceptor cassettes (A1, A2, and A3) each with its own non-homologous polyadenylation (pA) signals in a minimal backbone (AmpR and pUC ori).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    169600-plasmid-map-sequence-id-366610

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  37. pUC8 mouse IFN-gamma

    Plasmid
    #10955
    Depositor
    Howard Young
    Insert
    interferon-gamma (Ifng Mouse)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 14, 2006
    Availability
    Academic Institutions and Nonprofits only
    10955-plasmid-map-sequence-id-232759

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Showing: 681 - 720 of 4899 results