We narrowed to 17,234 results for: Emb;
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BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8
Plasmid#207031PurposeTet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
TagsmRuby3ExpressionYeastAvailable SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 8
Plasmid#36106DepositorAvailable SinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 2
Plasmid#36102DepositorAvailable SinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mNINJ1-K45Q-GFP
Plasmid#208777PurposeMSCV vector expressing K45Q mouse NINJ1DepositorAvailable SinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
mcherry Beta 1
Plasmid#36111DepositorAvailable SinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET17b-yebST
Plasmid#175804PurposeComplementation of yebST knockout in E. coli K-12DepositorInsertyebST
TagsNoneExpressionBacterialMutationsilent mutations to introduce restriction sitesPromoterT7Available SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
C1-MPAct(delta1-6)-mRuby3
Plasmid#155223PurposeF-actin bidning mutant of MPActDepositorInsertMutated version of F-tractin (aa 15-52 of rat ITPKA) fused to mRuby3 C-term tagged with the CaaX sequence derived from Kras4b
TagsmRuby3ExpressionMammalianMutationDeletion of first 6 AA from F-tractin (9-14 from …Available SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAF215
Plasmid#52402PurposeExpresses Flag-tagged mouse Arf1DepositorAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
C1-FT-mCitrine-EAAARx8-CaaX
Plasmid#155224PurposeMPAct with increased search radiusDepositorInsertF-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b
TagsmCitrineExpressionMammalianAvailable SinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-mNINJ1-D53A-GFP
Plasmid#208778PurposeMSCV vector expressing D53A mouse NINJ1DepositorInsertNINJ1 (Ninj1 Mouse)
UseRetroviralTagsGFPMutationChanged aspartic acid 53 to alanine (D53A)Available SinceAug. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK615
Plasmid#110609PurposeBTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9DepositorInsertsgRNA
UseSynthetic BiologyAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pJAF211
Plasmid#52407PurposeExpresses Flag-tagged mouse Arf6DepositorAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
mcherry Beta 4
Plasmid#36114DepositorAvailable SinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
mcherry Beta 3
Plasmid#36113DepositorAvailable SinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 13
Plasmid#36110DepositorAvailable SinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 12
Plasmid#36109DepositorAvailable SinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4A
Plasmid#115171PurposeExpresses membrane-targeted Sema4A with N-terminal myc tagDepositorInsertSema4A (Sema4a Mouse)
Tagsmembrane targeting sequence and mycExpressionMammalianMutationFirst 32 amino acids of Sema4A replaced with memb…PromoterCMVAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
TC1464
Plasmid#164881PurposeCMV-bpNLS-dCas13d-bpNLS-ADARdd, ADARdd embeded in loop1DepositorInsertdcas13d-ADARdd
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAF216
Plasmid#52405PurposeExpresses Flag-tagged mouse Arf4DepositorAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOmpT
Plasmid#112123PurposeBacterial expression outer membrane protein T (OmpT), wild typeDepositorInsertOmpT (ompT E. coli)
ExpressionBacterialMutationN-terminal signal sequence not included and repla…PromoterT7Available SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOmpW
Plasmid#112121PurposeBacterial expression of outer membrane protein W (OmpW), wild typeDepositorInsertOmpW (ompW E. coli)
ExpressionBacterialMutationN-terminal signal sequence not included and repla…PromoterT7Available SinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 7
Plasmid#36105DepositorInsertG protein subunit Gamma 7 (GNG7 Bovine)
TagsYFPExpressionMammalianMutationHIs tag between YFP and insertPromoterCMVAvailable SinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 4
Plasmid#36104DepositorAvailable SinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAL950
Plasmid#119748Purposeprecursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to CysteineDepositorInsertprecusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
TagsnoneExpressionBacterialMutationInternal NdeI site removed and mutations Alanine …PromoterT7Available SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS_AKAR5’
Plasmid#114141PurposeMammalian expression of the AKAR5’ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInsertAKAR5'
ExpressionMammalianAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJAF210
Plasmid#52406PurposeExpresses Flag-tagged mouse Arf5DepositorAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJAF214
Plasmid#52404PurposeExpresses Flag-tagged mouse Arf3DepositorAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet LRRK1 D1409A
Plasmid#241257PurposeFor exogenous expression of LRRK1 D1409A as an N-terminal fusion to mScarlet in mammalian cellsDepositorAvailable SinceJan. 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GG
Plasmid#213039PurposeAAV backbone for Golden Gate assembly of donor templates using BbsI/BpiI (overhangs AGCG and GCAA, respectively)DepositorTypeEmpty backboneUseAAV; Vector for golden gate assemblyTagsN/APromoterN/AAvailable SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-D384A Y387A-GFP
Plasmid#207024PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
TagsGFPExpressionYeastMutationD384A Y387APromoterpTEF1Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A I375A-GFP
Plasmid#207023PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
TagsGFPExpressionYeastMutationW371A I375APromoterpTEF1Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-Y387A-GFP
Plasmid#207022PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
TagsGFPExpressionYeastMutationY387APromoterpTEF1Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-D384A-GFP
Plasmid#207021PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
TagsGFPExpressionYeastMutationD384APromoterpTEF1Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-I375A-GFP
Plasmid#207020PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
TagsGFPExpressionYeastMutationI375APromoterpTEF1Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
BS-ZJLEU-PTEF1-HFL1-W371A-GFP
Plasmid#207019PurposeExpression of Hfl1 point mutant. Uses auxotrophic marker LEU2 (Saccharomyces cerevisiae).DepositorInsertHFL1
TagsGFPExpressionYeastMutationW371APromoterpTEF1Available SinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8-MET15
Plasmid#207055PurposeExpression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
Tags3xVSVExpressionYeastPromoterpATG8Available SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_Notch1-L24_sfGFP
Plasmid#207865PurposeYeast 2µ vector for expression of Suc2SP-Notch1-L24_100-sfGFP-GAL4DepositorInsertSuc2SP-Notch1-L24_100-sfGFP-GAL4 fusion protein
TagsStrep-TagIIExpressionBacterial and YeastAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_Notch1_sfGFP
Plasmid#207801PurposeYeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4DepositorInsertSuc2SP-Notch1_100-sfGFP-GAL4 fusion protein
TagsStrep-TagIIExpressionBacterial and YeastPromoterTEFAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEVA424::tsi5
Plasmid#192960PurposeExpresses the protein Tsi51-76 for biological function studies in a broad-spectrum of organismsDepositorInserttsi5
ExpressionBacterialAvailable SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29a(+)::D1141A
Plasmid#192963PurposeTest the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGLDepositorInsertTse5_D1141A
Tags9xhis + TEV tagExpressionBacterialMutationD1141AAvailable SinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29a(+)::9xhis-Tse5
Plasmid#192962PurposeHeterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CTDepositorInsertTse5
Tags9xhis + TEV tagExpressionBacterialAvailable SinceDec. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB6-Occludin
Plasmid#136637PurposeExpression of Occludin in mammalian cellsDepositorInsertOccludin (OCLN Chicken)
ExpressionMammalianAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB6-HAOccludin
Plasmid#136638PurposeExpression of HAOccludin in mammalian cellsDepositorAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB6-OccludinCT3
Plasmid#136639PurposeExpression of OccludinCT3 in mammalian cellsDepositorInsertOccludinCT3 (OCLN Chicken)
ExpressionMammalianMutationDeletion of C-terminal cytoplasid domainAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCB6-HAOccludinCT3
Plasmid#136640PurposeExpression of HAOccludinCT3 in mammalian cellsDepositorInsertHAOccludinCT3 (OCLN Chicken)
TagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationDeletion of C-terminal cytoplasid domainAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjPLP1e2-A39T
Plasmid#129752PurposeGene targeting in marmoset cellsDepositorAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjPLP1e2-P15L
Plasmid#129753PurposeGene targeting in marmoset cellsDepositorAvailable SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAL832
Plasmid#119305Purposeprecusor outer membrane protein A, ompA, truncated after amino acid 176DepositorInsertprecursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
TagsnoneExpressionBacterialMutationtruncated after amino acid 176, mutations Threoni…PromoterlacAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only
