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We narrowed to 917 results for: tac promoter

Showing: 341 - 360 of 917 results

  1. pN565

    Plasmid
    #49990
    Purpose
    expresses an attenuated T7 RNAP under an isopropyl β-d-1-thiogalactopyranoside (IPTG) inducible control
    Depositor
    Christopher Voigt
    Article
    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1
    Insert
    T7 RNAP*
    Use
    Synthetic Biology
    Tags
    umuD degradation tag
    Expression
    Bacterial
    Mutation
    R632S
    Promoter
    pTac-symmetric
    Available Since
    Jan. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    49990-plasmid-map-sequence-id-384231

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  2. ATP1A1_G6_T804N_Dual_pegRNA

    Plasmid
    #173200
    Purpose
    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified pegRNA from two independent U6 promoters. pU6-pegRNA-GG-acceptor-like plasmid
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G6 T804N pegRNA
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available Since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173200-plasmid-map-sequence-id-339515

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  3. pSC51

    Plasmid
    #104825
    Purpose
    CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter
    Depositor
    Robert Stupar
    Article
    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10
    Insert
    Glyma.12g075700, Glyma.11g145900
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available Since
    July 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    104825-plasmid-map-sequence-id-203148

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  4. pAP05

    Plasmid
    #186261
    Purpose
    sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter
    Depositor
    Yongku Cho
    Article
    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35
    Inserts
    sfGFP
    EL222 (ELI_04755 Erythrobacter litoralis)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…
    Available Since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    186261-plasmid-map-sequence-id-369315

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  7. EW263 CMV-TO-ADAR2dd(E488Q, T501A)-PUF-9R(FLP-IN)

    Plasmid
    #236126
    Purpose
    Plasmid encoding ADAR2dd attached to PUF-9R under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    ADAR2dd-PUF-9R (ADARB1 Human, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E488Q, T501A in ADAR2dd
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236126-plasmid-map-sequence-id-468414

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  8. EW499 CMV-TO-NZF-(GGS)x8[G1W S3D S6T G7L S9L G11M G14N G17D G19V G22W]-FRB(N2093W, T2098L)

    Plasmid
    #236146
    Purpose
    Plasmid encoding the NZF transcriptional activation domain attached by a deimmunized linker to FRB with N2093W and T098L mutations, under control of CMV promoter with two TetR binding sites
    Depositor
    Xiaojing Gao
    Article
    Wolfsberg et al bioRxiv 2025.02.24.639918
    Insert
    NZF-deImmunLink-FRB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N2093W, T2098L in FRB
    Promoter
    CMV promoter with two TetR binding sites
    Available Since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    236146-plasmid-map-sequence-id-468416

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  9. LT3GEPIR-NEK10shRNA2

    Plasmid
    #220557
    Purpose
    To inducibly knockdown NEK10 expression
    Depositor
    Sean Lee
    Insert
    NEK10 shRNA2 (NEK10 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220557-plasmid-map-sequence-id-437949

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  10. pZ8-T_dCas9

    Plasmid
    #74062
    Purpose
    pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR
    Depositor
    Timothy Lu
    Article
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    dcas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ptac
    Available Since
    April 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    74062-plasmid-map-sequence-id-137972

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  11. pBRα

    Plasmid
    #53731
    Purpose
    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    αNTD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available Since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53731-plasmid-map-sequence-id-83909

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  15. pCOTS-pyl-GFP(35TAG)

    Plasmid
    #92047
    Purpose
    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.
    Depositor
    Lital Alfonta
    Article
    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/
    Inserts
    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    Use
    Replicative expression plasmid for cyanobacteria …
    Tags
    Histag
    Expression
    Mutation
    Tyrosine in position 35 of the GFP was mutated f…
    Promoter
    LeuP (native S. elongatus promoter), PpsbII, and …
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92047-plasmid-map-sequence-id-194905

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  18. pNSSCPG

    Plasmid
    #183187
    Purpose
    PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p
    Depositor
    Hsiao-Chun Huang
    Article
    Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1
    Inserts
    superfolder GFP
    SpmX450 and T7 181+ fusion protein
    T7 RNAP 1-179 variant and SpmX450 fusion protein
    mRFP1 and popZ fusion protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    183187-plasmid-map-sequence-id-366657

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Showing: 341 - 360 of 917 results