We narrowed to 22,825 results for: Sis

1. pGEX2TK-Osh2-PH

   Plasmid

   #71370

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Osh2-PH
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa 277-398

   Promoter

   tac

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pRATIO2214

   Plasmid

   #141303

   Purpose

   Gateway cloning compatible dual fluorescent ratiometric binary vector

   Depositor

   David Nelson

   Article

   Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

   Type

   Empty backbone

   Tags

   mCerulean and mScarlet-I

   Expression

   Plant

   Promoter

   UBQ10 promoter

   Available Since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MDH1-PGK-GFP microRNA-10b

   Plasmid

   #16070

   Depositor

   Bob Weinberg

   Article

   Ma et al Nature. 2007 Oct 11. 449(7163):682-8.

   Insert

   microRNA-10b (MIR10B Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

4. hOtub1

   Plasmid

   #26959

   Depositor

   Cynthia Wolberger

   Article

   Wang et al J Mol Biol. 2009 Mar 6. 386(4):1011-23.

   Insert

   Otub1 (OTUB1 Human)
   

   Tags

   His6 (TEV cleavable)

   Expression

   Bacterial

   Available Since

   Jan. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

5. APT3-VAMP7

   Plasmid

   #168002

   Purpose

   positive selection PACE Accessory Plasmid

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   VAMP7-T3'PAP
   

   Use

   Synthetic Biology

   Available Since

   April 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. cavin-3-HA

   Plasmid

   #27711

   Depositor

   Ari Helenius

   Article

   Hayer et al Traffic. 2010 Mar . 11(3):361-82.

   Insert

   cavin-3 (CAVIN3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R8P

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL2_171

   Plasmid

   #199103

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pWKS1 ORI plus NGS barcode 17, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAGM53151

   Plasmid

   #169093

   Purpose

   Level 1 cloning vector for transient expression. Already contains the 35S promoter and the Nos terminator.

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

   Type

   Empty backbone

   Use

   Moclo cloning vector

   Expression

   Plant

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX2TK-Num1-PH

   Plasmid

   #71369

   Purpose

   bacterial expression of PH domain for phosphoinositide binding assay

   Depositor

   Mark Lemmon

   Article

   Yu et al Mol Cell. 2004 Mar 12;13(5):677-88.

   Insert

   Num1-PH
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Pleckstrin homology (PH) domain; aa2563-2692

   Promoter

   tac

   Available Since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pYFAC-pyroA

    Plasmid

    #168976

    Purpose

    Filamentous fungi vector with AMA1; 2µ replicon; URA3; AmpR ; pyroA

    Depositor

    Yit Heng Chooi

    Article

    Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

    Type

    Empty backbone

    Use

    Synthetic Biology; Expression in aspergillus nidu…

    Available Since

    Dec. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHis-zfCSF3a

    Plasmid

    #65612

    Purpose

    express soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli

    Depositor

    Petr Bartunek

    Article

    Stachura et al Blood. 2011 Aug 4;118(5):1274-82. doi: 1

    Insert

    Csf3a (csf3a Zebrafish)
    

    Tags

    (His)6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

12. Flag-ciBAR1

    Plasmid

    #200440

    Purpose

    Expresses Flag-tagged human ciBAR1 in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Li et al Mol Cell Biol. 2016 Oct 13;36(21):2668-2

    Insert

    ciBAR1
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Flag-ciBAR2

    Plasmid

    #200441

    Purpose

    Expresses Flag-tagged human ciBAR2 in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Li et al Mol Cell Biol. 2016 Oct 13;36(21):2668-2

    Insert

    ciBAR2
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p413TEF-Sir2-H364Y

    Plasmid

    #51743

    Purpose

    overexpression of Sir2 in S. cerevisiae (H364Y mutant), HIS3 marker

    Depositor

    Markus Ralser

    Article

    Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

    Insert

    Sir2 (SIR2 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    H364Y

    Promoter

    TEF1

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENTR-Rabin8

    Plasmid

    #24910

    Depositor

    Maxence Nachury

    Article

    Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

    Insert

    Rabin8 (RAB3IP Human)
    

    Use

    Gateway entry vector

    Available Since

    July 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-T1ER

    Plasmid

    #47928

    Purpose

    T1ER is a calcium sensor targeted to ER

    Depositor

    Tobias Meyer

    Article

    Bandara et al Sci Signal. 2013 Jul 9;6(283):ra56. doi:

    Insert

    T1ER
    

    Expression

    Mammalian

    Mutation

    KDEL

    Promoter

    CMV

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

17. LI F-G Spec

    Plasmid

    #104521

    Purpose

    LI resistance gene module

    Depositor

    Thomas Lahaye

    Article

    Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

    Insert

    Spectinomycin resistance gene
    

    Use

    Cloning vector

    Available Since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. YIplac204-pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL

    Plasmid

    #140153

    Purpose

    Expresses a regulatable vacuolar cargo for yeast at the TRP1 locus

    Depositor

    Benjamin Glick

    Article

    Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

    Insert

    pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPL
    

    Expression

    Yeast

    Promoter

    TPI1

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET15bLCFPT7

    Plasmid

    #53545

    Purpose

    Activation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant

    Depositor

    Lingchong You

    Article

    Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10

    Insert

    T7 RNAP
    

    Expression

    Bacterial

    Available Since

    July 28, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMaCTag-P31

    Plasmid

    #120042

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with V5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    V5
    

    Use

    Pcr template

    Tags

    V5

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pI1-MTU-DO-HIS

    Plasmid

    #160142

    Purpose

    Integrative vector for K. marxianus, targeting the LAC4 locus. Histidine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pI4-MTU-DO-HPH

    Plasmid

    #160216

    Purpose

    Integrative vector for K. marxianus, targeting integration site I4 described in (Rajkumar et al.,2019). Hygromycin selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Expression

    Yeast

    Promoter

    n/a

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET15b/GgVcl 1-1066 (full length)

    Plasmid

    #46171

    Depositor

    Susan Craig

    Article

    Bakolitsa et al Nature. 2004 Jul 29;430(6999):583-6. Epu

    Insert

    Vcl (VCL Chicken)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TRPML2-HA

    Plasmid

    #18828

    Depositor

    Craig Montell

    Article

    Venkatachalam et al J Biol Chem. 2006 Jun 23. 281(25):17517-

    Insert

    TRPML2 (Mcoln2 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 14, 2008

    Availability

    Academic Institutions and Nonprofits only

25. pMaCTag-12

    Plasmid

    #119991

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mScarlet

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet
    

    Use

    Pcr template

    Tags

    mScarlet

    Promoter

    None

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pUG-248

    Plasmid

    #104709

    Purpose

    Cloning Module

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    PUB22 C13A (PUB22 Mustard Weed)
    

    Use

    Synthetic Biology

    Mutation

    C13A, BpiI/BsaI sites removed by point-mutation

    Available Since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUG-21

    Plasmid

    #104707

    Purpose

    Cloning Module

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    PUB22 WT (PUB22 Mustard Weed)
    

    Use

    Synthetic Biology

    Mutation

    BpiI/BsaI sites removed by point-mutation

    Available Since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUG-UBC1

    Plasmid

    #104666

    Purpose

    Cloning Module

    Depositor

    Marco Trujillo

    Article

    Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

    Insert

    AtUBC1 (UBC1 Mustard Weed)
    

    Use

    Synthetic Biology

    Available Since

    Feb. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHX_gRNA_hPAX7_6-9S

    Plasmid

    #160453

    Purpose

    sgRNA targeting human PAX7 3' UTR with 17bp spacer

    Depositor

    April Pyle

    Article

    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

    Insert

    gRNA_hPAX7_6-9S
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPOTv7-blast-eGFP

    Plasmid

    #179781

    Purpose

    Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

    Depositor

    Keith Gull, Samuel Dean

    Article

    Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

    Insert

    eGFP
    

    Use

    Unspecified

    Promoter

    read through

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCfB4666

    Plasmid

    #106144

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)

    Plasmid

    #173453

    Purpose

    Contains mCherry-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mCherry-mNeonGreen
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MSCVhyg-F-Axin1

    Plasmid

    #24928

    Depositor

    Kai Ge

    Article

    Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

    Insert

    Axin1 (AXIN1 Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    June 27, 2010

    Availability

    Academic Institutions and Nonprofits only

34. pWH-DI

    Plasmid

    #47385

    Purpose

    Expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

    Depositor

    Heng-Phon Too

    Article

    Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

    Inserts

    dxs
    idi
    

    Expression

    Bacterial

    Promoter

    PxylA

    Available Since

    Oct. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

35. K18iresCreER-T2

    Plasmid

    #44580

    Depositor

    Robert Oshima

    Article

    Wen et al Genesis. 2003 Feb;35(2):100-6.

    Insert

    keratin 18 gene (KRT18 Human)
    

    Use

    Mouse Targeting

    Tags

    IRES_CreER-T2

    Available Since

    June 18, 2013

    Availability

    Academic Institutions and Nonprofits only

36. pThuS

    Plasmid

    #171674

    Purpose

    ThuS expression in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Fujinami et al Cell Chem Biol. 2021 Dec 16;28(12):1740-

    Insert

    Glycosyltransferase ThuS
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCDNA4-Myc-HisB-Sirt3

    Plasmid

    #24918

    Depositor

    Toren Finkel

    Article

    Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

    Insert

    Sirt3 (SIRT3 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

38. pHR-SIN-PTEN-WT

    Plasmid

    #30370

    Depositor

    Todd Waldman

    Article

    Kim et al Mol Cell Biol. 2011 Jul . 31(13):2756-71

    Insert

    PTEN (PTEN Human)
    

    Expression

    Mammalian

    Mutation

    wild type

    Available Since

    Aug. 11, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pMB62

    Plasmid

    #47944

    Purpose

    Expresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, egl-13 NLS, and mEGFP

    Expression

    Worm

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRATIO1267

    Plasmid

    #141301

    Purpose

    Gateway cloning compatible dual bioluminescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Tags

    gLUC and redLUC

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge