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  1. MADR pDonor smFP-V5 (dark) WPRE

    Plasmid
    #131006
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-V5, a cytoplasmic fluorescent protein which includes multiple V5 epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    V5 - 10 total of V5 epitope tag
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131006-plasmid-map-sequence-id-305181

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  4. MADR pDonor smFP-Myc (dark) WPRE

    Plasmid
    #130987
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-Myc, a cytoplasmic fluorescent protein which includes multiple Myc epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-Myc WPRE
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    Myc - 10 total Myc tags
    Mutation
    "dark" variant with GGG fluorophore ver…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    130987-plasmid-map-sequence-id-305000

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  7. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(PAMmut)

    Plasmid
    #176139
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176139-plasmid-map-sequence-id-353012

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  8. MADR pDonor smFP-FLAG (dark) WPRE

    Plasmid
    #131005
    Purpose
    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-FLAG, a cytoplasmic fluorescent protein which includes multiple FLAG epitope tags
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    smFP-FLAG WPRE
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    FLAG - 10 total FLAG tags
    Mutation
    "dark" variant with GGG fluorphore comp…
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    131005-plasmid-map-sequence-id-305005

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  9. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131462
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A K27M, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131462-plasmid-map-sequence-id-305183

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  10. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131461
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131461-plasmid-map-sequence-id-305184

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  11. MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131463
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A G34R, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    131463-plasmid-map-sequence-id-305001

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  14. MADR pDonor TagBFP2-V5-HRAS G12VWPRE

    Plasmid
    #129420
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
    Use
    Mosaic analysis for dual recombinase-mediated cas…
    Tags
    TagBFP2-V5 tag
    Mutation
    G12V
    Promoter
    none (3x polyA to mitigate episomal expression)
    Available Since
    Sept. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    129420-plasmid-map-sequence-id-257068

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  15. Lenti-CMV-EGFP-MTHFD1 K56R-WPRE

    Plasmid
    #250371
    Purpose
    Lentiviral expression of human MTHFD1 carrying K56R mutation with N-terminal EGFP and puromycin resistance cassette under PGK promoter. Mutation was generated by Q5 site-directed mutagenesis.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    MTHFD1 (MTHFD1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    K56R
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250371-plasmid-map-sequence-id-498985

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  16. Lenti-CMV-EGFP-MTHFD1 K384E-WPRE

    Plasmid
    #250372
    Purpose
    Lentiviral expression of human MTHFD1 carrying K384E mutation with N-terminal EGFP and puromycin resistance cassette under PGK promoter. Mutation was generated by Q5 site-directed mutagenesis.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    MTHFD1 (MTHFD1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    K384E
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250372-plasmid-map-sequence-id-498984

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  20. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)

    Plasmid
    #176141
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutation in Asp256 to Ala, a…
    Promoter
    EF1A
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176141-plasmid-map-sequence-id-353041

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  21. EC71174

    Plasmid
    #154067
    Purpose
    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Expression
    Bacterial and Plant
    Mutation
    The Arabidopsis thaliana U5 small nuclear ribonuc…
    Promoter
    35S promoter, Hordeum vulgare HSP 17 promoter use…
    Available Since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154067-plasmid-map-sequence-id-313091

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  22. pUAP1

    Plasmid
    #63674
    Purpose
    Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.
    Depositor
    Nicola Patron
    Article
    Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np
    Insert
    RFP cloning selection cassette
    Use
    Synthetic Biology
    Available Since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63674-plasmid-map-sequence-id-106088

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  24. MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

    Plasmid
    #131730
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    TagBFP2-V5-HRAS G12V (HRAS Entacmaea quadricolor (TagBFP), Human)
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    TagBFP2-V5 tag
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    131730-plasmid-map-sequence-id-318177

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  26. Lenti-CMV-mCherry-GSG-P2A-NUDT5 G150V-WPRE

    Plasmid
    #250402
    Purpose
    Lentiviral expression of human NUDT5 G150V with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    NUDT5 (NUDT5 Human)
    Use
    Lentiviral
    Tags
    mCherry-GSG-P2A
    Expression
    Mammalian
    Mutation
    G150V
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250402-plasmid-map-sequence-id-498983

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  27. Lenti-CMV-mCherry-GSG-P2A-NUDT5 P158I-WPRE

    Plasmid
    #250403
    Purpose
    Lentiviral expression of human NUDT5 P158I with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    NUDT5 (NUDT5 Human)
    Use
    Lentiviral
    Tags
    mCherry-GSG-P2A
    Expression
    Mammalian
    Mutation
    P158I
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250403-plasmid-map-sequence-id-498982

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  29. Lenti-CMV-mCherry-GSG-P2A-NUDT5 W28A-WPRE

    Plasmid
    #250389
    Purpose
    Lentiviral expression of human NUDT5 W28A with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    NUDT5 (NUDT5 Human)
    Use
    Lentiviral
    Tags
    mCherry-GSG-P2A
    Expression
    Mammalian
    Mutation
    W28A
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250389-plasmid-map-sequence-id-498981

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  30. Lenti-CMV-mCherry-GSG-P2A-NUDT5 T45D-WPRE

    Plasmid
    #250388
    Purpose
    Lentiviral expression of human NUDT5 T45D with N-terminal mCherry seperated by a flexible GSG-linker and P2Aunder CMV promoter, as well as a puromycin resistance cassette under PGK promoter.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    NUDT5 (NUDT5 Human)
    Use
    Lentiviral
    Tags
    mCherry-GSG-P2A
    Expression
    Mammalian
    Mutation
    T45D
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250388-plasmid-map-sequence-id-498976

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  31. Lenti-CMV-mCherry-GSG-P2A-NUDT5 E112Q-WPRE

    Plasmid
    #250391
    Purpose
    Lentiviral expression of human NUDT5 E112Q with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    NUDT5 (NUDT5 Human)
    Use
    Lentiviral
    Tags
    mCherry-GSG-P2A
    Expression
    Mammalian
    Mutation
    E112Q
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250391-plasmid-map-sequence-id-498977

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  32. Lenti-CMV-mCherry-GSG-P2A-NUDT5 T45A-WPRE

    Plasmid
    #250387
    Purpose
    Lentiviral expression of human NUDT5 T45A with N-terminal mCherry seperated by a flexible GSG-linker and P2Aunder CMV promoter, as well as a puromycin resistance cassette under PGK promoter.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    NUDT5 (NUDT5 Human)
    Use
    Lentiviral
    Tags
    mCherry-GSG-P2A
    Expression
    Mammalian
    Mutation
    T45A
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250387-plasmid-map-sequence-id-498973

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  34. Lenti-CMV-mCherry-GSG-P2A-NUDT5 P66L-WPRE

    Plasmid
    #250393
    Purpose
    Lentiviral expression of human NUDT5 P66L with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    NUDT5 (NUDT5 Human)
    Use
    Lentiviral
    Tags
    mCherry-GSG-P2A
    Expression
    Mammalian
    Mutation
    P66L
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250393-plasmid-map-sequence-id-498970

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  35. Lenti-CMV-mCherry-GSG-P2A-NUDT5 K175M-WPRE

    Plasmid
    #250405
    Purpose
    Lentiviral expression of human NUDT5 K175M with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    NUDT5 (NUDT5 Human)
    Use
    Lentiviral
    Tags
    mCherry-GSG-P2A
    Expression
    Mammalian
    Mutation
    K175M
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250405-plasmid-map-sequence-id-498972

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  37. Lenti-CMV-mCherry-GSG-P2A-NUDT5 C76Y-WPRE

    Plasmid
    #250394
    Purpose
    Lentiviral expression of human NUDT5 C76Y with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    NUDT5 (NUDT5 Human)
    Use
    Lentiviral
    Tags
    mCherry-GSG-P2A
    Expression
    Mammalian
    Mutation
    C76Y
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250394-plasmid-map-sequence-id-498967

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  39. Lenti-CMV-mCherry-GSG-P2A-NUDT5-WPRE

    Plasmid
    #250386
    Purpose
    Lentiviral expression of human NUDT5 with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    NUDT5 (NUDT5 Human)
    Use
    Lentiviral
    Tags
    mCherry-GSG-P2A
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250386-plasmid-map-sequence-id-498965

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  40. Lenti-CMV-mCherry-GSG-P2A-NUDT5 Y74E-WPRE

    Plasmid
    #250397
    Purpose
    Lentiviral expression of human NUDT5 Y74E with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    NUDT5 (NUDT5 Human)
    Use
    Lentiviral
    Tags
    mCherry-GSG-P2A
    Expression
    Mammalian
    Mutation
    Y74E
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250397-plasmid-map-sequence-id-498962

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  43. Lenti-CMV-mCherry-GSG-P2A-NUDT5 Y74F-WPRE

    Plasmid
    #250398
    Purpose
    Lentiviral expression of human NUDT5 Y74F with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    NUDT5 (NUDT5 Human)
    Use
    Lentiviral
    Tags
    mCherry-GSG-P2A
    Expression
    Mammalian
    Mutation
    Y74F
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250398-plasmid-map-sequence-id-498956

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  44. Lenti-CMV-mCherry-GSG-P2A-NUDT5 P158T-WPRE

    Plasmid
    #250404
    Purpose
    Lentiviral expression of human NUDT5 P158T with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    NUDT5 (NUDT5 Human)
    Use
    Lentiviral
    Tags
    mCherry-GSG-P2A
    Expression
    Mammalian
    Mutation
    P158T
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250404-plasmid-map-sequence-id-498955

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  47. PB-EEA-5g-OSK2M2L1-PGK-Puro

    Plasmid
    #102909
    Purpose
    PiggyBac transposon system construct for expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters and 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    EEA-5g-OSK2M2L1-gRNAs-PGK-Puro (POU5F1, SOX2, KLF4, MYC, LIN28A Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102909-plasmid-map-sequence-id-200136

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  49. Lenti-CMV-mCherry-GSG-P2A-NUDT5 W28A W46A Y74F-WPRE

    Plasmid
    #250400
    Purpose
    Lentiviral expression of human NUDT5 W28A W46A Y74F with N-terminal mCherry seperated by a GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    NUDT5 (NUDT5 Human)
    Use
    Lentiviral
    Tags
    mCherry-GSG-P2A
    Expression
    Mammalian
    Mutation
    W28A, W46A, Y74F
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250400-plasmid-map-sequence-id-498975

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  50. Lenti-CMV-mCherry-GSG-P2A-NUDT5 Y74F E112Q-WPRE

    Plasmid
    #250401
    Purpose
    Lentiviral expression of human NUDT5 Y74F E112Q with N-terminal mCherry seperated by a flexible GSG-linker and P2A under CMV promoter, as well as a puromycin resistance cassette under PGK promoter.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    NUDT5 (NUDT5 Human)
    Use
    Lentiviral
    Tags
    mCherry-GSG-P2A
    Expression
    Mammalian
    Mutation
    Y74F, E112Q
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250401-plasmid-map-sequence-id-498971

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