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Showing: 8951 - 9000 of 23360 results

  2. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid
    #174140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174140-plasmid-map-sequence-id-341918

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  3. pCfB6627

    Plasmid
    #106159
    Purpose
    EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6682 into Yarrowia lipolytica chromosomal location IntC_2, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Insert
    unknown
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106159-plasmid-map-sequence-id-393637

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  6. pRS416gT-GalL-Cas9

    Plasmid
    #79903
    Purpose
    pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression
    Depositor
    Ronald Davis
    Article
    Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw
    Inserts
    Cas9
    guide RNA (gRNA)
    Tetracycline Repressor
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    GPM1 promoter, GalL, and RPR1 promoter with TetO …
    Available since
    July 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    79903-plasmid-map-sequence-id-157872

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  10. pXGFPC-5 Pxyl-dcas9 (Spa)

    Plasmid
    #133318
    Purpose
    expresses dcas9 (Streptococcus pasteurianus); repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    dcas9 (Streptococcus pasteurianus)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    xylose
    Available since
    Feb. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    133318-plasmid-map-sequence-id-266833

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  12. pMOD6-pGRR-r11-r10-yeGFP

    Plasmid
    #74368
    Purpose
    pGRR NOR gate promoter responsive to gRNA-r11 and gRNA-r10. Integrates in the URA locus of W303 and recovers URA function.
    Depositor
    Eric Klavins
    Article
    Gander et al bioRxiv 041871
    Insert
    yeGFP
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pGRR-r11-r10
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74368-plasmid-map-sequence-id-141319

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  17. pJEC581

    Plasmid
    #174369
    Purpose
    RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.
    Depositor
    James Chappell
    Article
    Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793
    Insert
    RFP with PAM rich sequence upstream promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23117
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    174369-plasmid-map-sequence-id-345702

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  20. pU6_(GLuc)_INT[Spinach2]

    Plasmid
    #68430
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing the "Spinach2" aptamer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68430-plasmid-map-sequence-id-120208

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  31. pARNTL.1.0-gDNA

    Plasmid
    #112480
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ARNTL
    Depositor
    Kevin White
    Insert
    ARNTL (BMAL1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112480-plasmid-map-sequence-id-230516

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  35. pCfB6677

    Plasmid
    #106155
    Purpose
    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6632, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106155-plasmid-map-sequence-id-206140

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  36. pLib6.6

    Plasmid
    #176652
    Purpose
    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
    Depositor
    Norbert Perrimon
    Article
    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:
    Type
    Empty backbone
    Use
    Crispr
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176652-plasmid-map-sequence-id-348545

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  42. pLib6.4B

    Plasmid
    #176665
    Purpose
    Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
    Depositor
    Norbert Perrimon
    Article
    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:
    Type
    Empty backbone
    Use
    Crispr
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176665-plasmid-map-sequence-id-348636

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  47. pCfB6684

    Plasmid
    #106154
    Purpose
    EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6631, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106154-plasmid-map-sequence-id-206237

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Showing: 8951 - 9000 of 23360 results