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Addgene

We narrowed to 8,240 results for: FIE

Showing: 7201 - 7240 of 8240 results
  1. pPRISM-QF2-he1.1:YFP

    Plasmid
    #184819
    Purpose
    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.
    Depositor
    Insert
    QF2-pA-he1.1:YFP
    Use
    CRISPR; Backbone for cloning of homology arms (fo…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pPRISM-QF2-he1.1:YFP-rln3a-HA

    Plasmid
    #184820
    Purpose
    Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.
    Depositor
    Insert
    rln3a-HA
    Use
    CRISPR; For integration of qf2 by homology-direct…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 29, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pJL1-MBP-DQNAT

    Plasmid
    #198215
    Purpose
    pJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag
    Depositor
    Insert
    MBP_DQNAT
    Use
    Tags
    6x His Tag and DQNAT glycosylation tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pJL1-PD-DQNAT

    Plasmid
    #198216
    Purpose
    pJL1 plasmid encoding H. influenzae protein D (PD) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag
    Depositor
    Insert
    PD-DQNAT
    Use
    Tags
    6x His Tag and DQNAT glycosylation tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. EGFP–acVHH–PB

    Plasmid
    #169685
    Purpose
    An anti-caffeine nanobody responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Insert
    anti-caffeine nanobody
    Use
    Tags
    modified STIM1-PB tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 7201 - 7240 of 8240 results