4,143 results

1. Flag-HA-USP46

   Plasmid

   #22584

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP46 (USP46 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. FCK-ChR2-GFP

   Plasmid

   #15814

   Purpose

   Lentiviral expression of ChR2-GFP for neuronal excitation

   Depositor

   Edward Boyden

   Article

   Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

   Insert

   ChR2
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDULE-ABK

   Plasmid

   #49086

   Purpose

   A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments

   Depositor

   Peter Schultz

   Article

   Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.

   Inserts

   tRNA synthetase
   tRNA for a photocrosslinking amino acid
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   L274M, C313A, Y349F

   Promoter

   GlnRS and lpp

   Available since

   Nov. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-SENP1m

   Plasmid

   #17358

   Depositor

   Edward Yeh

   Article

   Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

   Insert

   SENP1 (SENP1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   R630L, K631M

   Promoter

   Available since

   March 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. HSF1-GFPN3

   Plasmid

   #32538

   Depositor

   Stuart Calderwood

   Article

   Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

   Insert

   HSF1 (HSF1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   see depositor comments below

   Promoter

   CMV

   Available since

   Oct. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG.PKCepsilon

   Plasmid

   #10795

   Depositor

   Alex Toker

   Article

   Cenni et al Biochem J. 2002 May 1. 363(Pt 3):537-45.

   Insert

   PKC epsilon (PRKCE Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-HA-JMJD2A

   Plasmid

   #24180

   Purpose

   Mammalian expression of human JMJD2A with HA tag

   Depositor

   Kristian Helin

   Article

   Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

   Insert

   JMJD2A (KDM4A Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-EF1a-BbChT

   Plasmid

   #45186

   Depositor

   Dawen Cai, Joshua Sanes

   Article

   Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

   Has Service

   AAV9

   Insert

   pAAV-Ef1a-Brainbow/mCherry/mTFP-WPRE
   

   Use

   AAV; Adeno-associated viral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human EF1a

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR207 SARS-CoV-2 M

   Plasmid

   #141274

   Purpose

   Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Has Service

   Cloning Grade DNA

   Insert

   M (M SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pBABE-hygro p53 DD

    Plasmid

    #9058

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    p53 DD (Trp53 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    DD. Dominant negative mutant carries deletion of…

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGFP-HBx

    Plasmid

    #24931

    Depositor

    Xin Wang

    Article

    Forgues et al J Biol Chem. 2001 Jun 22. 276(25):22797-

    Insert

    HBx
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSico PGK puro

    Plasmid

    #11586

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-EF1a-BbTagBY

    Plasmid

    #45185

    Depositor

    Dawen Cai, Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Has Service

    AAV9

    Insert

    pAAV-EF1a-Brainbow-invert tagBFP-EYFP-WPRE
    

    Use

    AAV; Adeno-associated viral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1a

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-Myc CDC4 WT*

    Plasmid

    #16652

    Depositor

    Bert Vogelstein

    Article

    Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

    Insert

    CDC4 (FBXW7 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    *Note that this insert is only WT relative to the…

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMKO.1 puro GFP shRNA

    Plasmid

    #10675

    Purpose

    Negative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.

    Depositor

    William Hahn

    Article

    Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

    Insert

    GFP shRNA
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pmEGFP-1

    Plasmid

    #36409

    Depositor

    Benjamin Glick

    Type

    Empty backbone

    Use

    Fluorescent protein cassette for sub-cloning

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    no promoter

    Available since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. CX-nestinGFPas

    Plasmid

    #32401

    Depositor

    Henryk Zulewski, Patrick Salmon

    Article

    Eberhardt et al Biochem Biophys Res Commun. 2006 Jul 7;3

    Insert

    nestin promoter/enhancer GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYPQ152

    Plasmid

    #69303

    Purpose

    Gateway entry vector with pco-dCas9-VP64

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pco-dCas9-VP64 (Plant codon-optimized)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    VP64 activator fusion; NLS fusion

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP-BLM

    Plasmid

    #80070

    Purpose

    Expresses GFP-tagged BLM helicase in mammalian cells (EGFP-C1 backbone)

    Depositor

    Nathan Ellis

    Article

    Hu et al Hum Mol Genet. 2001 Jun 1;10(12):1287-98

    Insert

    BLM (BLM Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    If correct ID, the cDNA is from HeLa and has char…

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA 3.1(-) mouse C/EBP beta (LAP)

    Plasmid

    #12557

    Depositor

    Peter Johnson

    Insert

    CCAAT/Enhancer-binding Protein beta (Cebpb Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge