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pBABE-puro-mTwist

Plasmid

#1783

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

Twist (Twist1 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp8 C360A

Plasmid

#11818

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 8 catalytic mutant (CASP8 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C360A

Promoter

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-BAX

Plasmid

#16587

Depositor

Bert Vogelstein

Article

Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Insert

BAX alpha (BAX Human)

Use

Tags

2x HA

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE7.9

Plasmid

#102918

Purpose

expresses ABE7.9 in mammalian cells

Depositor

David Liu

Article

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Insert

ABE7.9

Use

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Atg12

Plasmid

#24923

Depositor

Toren Finkel

Article

Lee et al J Biol Chem. 2009 Mar 6. 284(10):6322-8.

Insert

Atg12 (ATG12 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

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T7-RelA

Plasmid

#21984

Depositor

Warner Greene

Article

Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

Insert

NFkB p65 subunit (RELA Human)

Use

Tags

T7 gp10

Expression

Mammalian

Mutation

For more details for this expression construct,…

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pCMVInt

Plasmid

#18935

Depositor

Michele Calos

Article

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

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pLNCX chick src Y527F

Plasmid

#13660

Depositor

Joan Brugge

Insert

src Y527F (SRC Chicken)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Y527F. Constitutive active mutant.

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

Plasmid

#64218

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E1B

Expression

Mammalian

Mutation

Promoter

CBh and U6

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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YFP-FKBP (YF)

Plasmid

#20175

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP (YF): (FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

FKBP was taken out from pC4M-F2E with a flanking …

Promoter

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-CFP II (pMIC II)

Plasmid

#52109

Purpose

a bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

Depositor

Dario Vignali

Article

Holst et al Nat Protoc. 2006;1(1):406-17.

Type

Empty backbone

Use

Retroviral

Tags

IRES-ECFP

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Fos WT

Plasmid

#8966

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only

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pCI-neo-sOVA

Plasmid

#25098

Depositor

Maria Castro

Article

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

soluble ovalbumin

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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Ai6

Plasmid

#22798

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

Insert

CAG-Floxed ZsGreen

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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Nanog5P reporter

Plasmid

#16337

Depositor

Austin Cooney

Article

Gu et al Mol Cell Biol. 2005 Oct . 25(19):8507-19

Insert

Nanog promoter (Nanog Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Mouse Nanog promoter

Available since

Dec. 6, 2007

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Sox9

Plasmid

#41080

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell Stem Cell. 2012 Sep 7;11(3):373-86.

Insert

Sox9 (Sox9 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TetO + minimal CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-H2B-SunTag24x_v1

Plasmid

#60911

Purpose

Enocdes a histone H2B fused to the SunTag for single molecule imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

H2B

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO-RB1-shRNA63

Plasmid

#25641

Purpose

3rd generation lentiviral vector

Depositor

Todd Waldman

Article

Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Insert

RB1-shRNA 63 (RB1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pIREShyg-PA-GFP-alpha Tubulin

Plasmid

#12296

Depositor

Patricia Wadsworth

Article

Tulu et al Curr Biol. 2003 Oct 28. 13(21):1894-9.

Insert

alpha Tubulin (TUBA1B Human)

Use

Tags

PA-GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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