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actc1b-mKate2-GPI-pA

Plasmid

#109490

Purpose

Muscle specific zebrafish expression of mKate2 fused to the 5' and 3' sequences from zebrafish folate receptor 1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-GPI-pA

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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FAK

Plasmid

#186141

Purpose

For expresseion of recombinant FAK using baccolovirus in SF9 cells

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

PTK2 (PTK2 Mouse)

Tags

His6

Expression

Insect

Promoter

polyhedrin promoter

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab5b

Plasmid

#109650

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab5b

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab5b (rab5b Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab11bb

Plasmid

#109597

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab11bb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab11bb (rab11bb Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab11a

Plasmid

#109594

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab11a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab11a (rab11a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab11al

Plasmid

#109595

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab11al

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab11al (rab11al Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDGB3_omega1

Plasmid

#68238

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha2

Plasmid

#68229

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmSmaug-V5His6_C

Plasmid

#146040

Purpose

Insect Expression of DmSmaug

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

DmSmaug (smg Fly)

Expression

Insect

Mutation

10 silent mutations and a deletion of the AA 961-…

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt9B-V5

Plasmid

#35938

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt9B (WNT9B Human)

Tags

V5

Expression

Mammalian

Mutation

SNP Met106-->Thr

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 PSMC1

Plasmid

#67087

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PSMC1 (PSMC1 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

E275G, I287V and R294G

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pMEH295 NLS dTomato 1x wild type MS2 loop

Plasmid

#168214

Purpose

Mammalian expression of nuclear-localized dTomato with 1 wild type MS2 loop

Depositor

Joshua Leonard

Article

Hung et al J Extracell Vesicles. 2016 May 13;5:3102

Insert

NLS dTomato

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-zeo small T 110

Plasmid

#9063

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

SV40 small T Antigen 110 (SV40gp7 simian virus)

Use

Retroviral

Expression

Mammalian

Mutation

Contains only the N-termial 110 amino acids

Available Since

Sept. 23, 2005

Availability

Academic Institutions and Nonprofits only
RhoD

Plasmid

#55700

Purpose

expression of mouse RhoD

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoD (Rhod Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pJUMP18-B0034-MV_RN

Plasmid

#127017

Purpose

Basic Part RN- RBS+N-terminus; pET vector Ribosome Binding Site + Met-Val N-terminus start.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part B0034m-MV_RN

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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CXCL5 toehold switch sensor

Plasmid

#111907

Purpose

Toehold switch sensor to detect CXCL5 mRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

CXCL5 toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag 3A/myc-M1L195V

Plasmid

#87716

Purpose

Expresses myc-M1L195V spastin isoform in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

Insert

SPAST (SPAST Human)

Tags

Myc

Expression

Mammalian

Mutation

M1 isoform; L195V

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pRG711_lexO-AscI_LexA-TF_HIS3MX

Plasmid

#154820

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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KJ101: pMVP (R4-R3) osTir1

Plasmid

#121742

Purpose

pMVP R4-R3 entry plasmid, contains osTir1 for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

osTir1

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

9x myc epitope tag

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-RhoAa

Plasmid

#109577

Purpose

Multisite gateway vector for 3' tagging with RhoAa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAa (rhoaa Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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IG401: pMVP (L1-R5) RIP

Plasmid

#121694

Purpose

pMVP L1-R5 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

RIP

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IG603: pMVP (L1-L4) TRE promoter

Plasmid

#121691

Purpose

pMVP L1-L4 entry plasmid, contains TRE promoter for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TRE promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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rGSKmyc-pXT7

Plasmid

#15492

Depositor

Sergei Sokol

Article

Dominguez et al Proc Natl Acad Sci U S A. 1995 Aug 29. 9

Insert

GSK-3 beta (Gsk3b Rat)

Use

Xenopus rna expression

Tags

myc

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pHCKan-yibDp-mdlC

Plasmid

#134590

Purpose

Low Phosphate inducible mdlC benzylformate decarboxylase

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

mdlC (benzylformate decarboxylase) C -terminal his tag

Tags

6x-His

Expression

Bacterial

Promoter

yibDp

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAflaB

Plasmid

#149642

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLX-V5-TRAF2 S11A-puro

Plasmid

#44112

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S11A (TRAF2 Human)

Use

Lentiviral

Tags

V5

Mutation

Serine 11 to Alanine

Available Since

July 22, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 Hmga2 m2

Plasmid

#14790

Depositor

David Bartel

Article

Mayr et al Science. 2007 Mar 16. 315(5818):1576-9.

Insert

Hmga2 m2 (Hmga2 Mouse)

Expression

Mammalian

Mutation

Hmga2 m2 mutant (see article and author's ma…

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
pBbdCas9S_Psyn-sgRNAmreB

Plasmid

#149653

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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RAP2C

Plasmid

#55668

Purpose

expression of mouse RAP2C

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RAP2C (Rap2c Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
Pr2-T7RNAP

Plasmid

#67739

Purpose

OR2-OR1-P2 promoter expressing T7 RNAP

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-T7RNAP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr2

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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