We narrowed to 10,449 results for: T7

1. pET21a - TDR

   Plasmid

   #89627

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   thiol dependent reductase
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET21a - NFDD

   Plasmid

   #89624

   Purpose

   Expresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   NAD/FAD dependent dehydrogenase
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET151-Vps4(101-437) L151D

   Plasmid

   #87736

   Purpose

   Bacterial expression of a monomeric variant of the S. cerevisiae Vps4 AAA ATPase cassette

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100, mutated Leu 151 to Asp

   Promoter

   T7

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pC3R112C/T180C

   Plasmid

   #85088

   Purpose

   Expresses PCNA3 variant (R112C/T180C) fused to P450cam (CYP101A1) in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   The R112C/T180C variant of PCNA3 fused to P450cam
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C in PCNA3

   Promoter

   T7 promoter

   Available since

   Dec. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p3R112C/T180C-GG

   Plasmid

   #85093

   Purpose

   Host vector to fuse a gene at the C-terminus of PCNA3 variant (R112C/T180C)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   DNA polymerase sliding clamp 3
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Arginine 112 to Cysteine and Threonine 18…

   Promoter

   T7 promoter

   Available since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-Hook3 Q147A, M151A, I154A

   Plasmid

   #74616

   Purpose

   Expresses a triple mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, Q147A, M151A, I154A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Q147A, M151A, I154A

   Promoter

   T7

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-Hook3 D102A, E108A

   Plasmid

   #74618

   Purpose

   Expresses a double mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, D102A, E108A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   D102A, E108A

   Promoter

   T7

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-Hook3 N68A, W69A, K77A

   Plasmid

   #74619

   Purpose

   Expresses a triple mutant truncation of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-239, N68A, W69A, K77A (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRSETb-rsGreen0.9

   Plasmid

   #78179

   Purpose

   Expresses-rsGreen0.9 in E. coli strains.

   Depositor

   Peter Dedecker

   Article

   Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

   Insert

   rsGreen0.9
   

   Use

   Tags

   6xHis-tag; Xpress tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSETb-rsGreen0.7b

    Plasmid

    #78178

    Purpose

    Expresses rsGreen0.7b in E. coli strains.

    Depositor

    Peter Dedecker

    Article

    Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

    Insert

    rsGreen0.7b
    

    Use

    Tags

    6xHis-tag; Xpress tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJK516

    Plasmid

    #71578

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Gln mutant (AarCH6-E435Q)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-435 to glutamine

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJK524

    Plasmid

    #71580

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Asn347>Ala mutant (AarCH6-N347A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes asparagine-347 to alanine

    Promoter

    T7

    Available since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJK513

    Plasmid

    #71576

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu294>Ala mutant (AarCH6-E294A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-294 to alanine

    Promoter

    T7

    Available since

    April 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETM6-Vv4CL-PhCHS-MsCHI

    Plasmid

    #73400

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Vv4CL
    PhCHS
    MsCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pETM6-Vv4CL-CmCHS-CmCHI

    Plasmid

    #73403

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Vv4CL
    CmCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETM6-Pc4CL-CmCHS-CmCHI

    Plasmid

    #73399

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    Pc4CL
    CmCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK287

    Plasmid

    #71120

    Purpose

    Produces Acetobacter aceti 1023 formyl-CoA:oxalate CoA-transferase (H6UctB) in E. coli

    Depositor

    T. J. Kappock

    Article

    Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

    Insert

    formyl-CoA:oxalate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK564

    Plasmid

    #71581

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Ser71>Ala mutant (AarCH6-S71A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes serine-71 to alanine

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK517

    Plasmid

    #71579

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Asp mutant (AarCH6-E435D)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamate-435 to aspartate

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK514

    Plasmid

    #71577

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Glu435>Ala mutant (AarCH6-E435A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes glutamine-435 to alanine

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJK512

    Plasmid

    #71575

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag and Arg228>Glu mutant (AarCH6-R228E)

    Depositor

    T. J. Kappock

    Article

    Mullins et al Biochemistry. 2012 Oct 23;51(42):8422-34

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    changes arginine-228 to glutamate

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJK360

    Plasmid

    #71571

    Purpose

    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)

    Depositor

    T. J. Kappock

    Article

    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

    Insert

    acetyl-CoA:oxalate CoA-transferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changes threonine-2 to alanine

    Promoter

    T7

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJK385

    Plasmid

    #71572

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6)

    Depositor

    T. J. Kappock

    Article

    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJK174

    Plasmid

    #71835

    Purpose

    Expression of Acetobacter aceti 1023 PurE and PurK in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Inserts

    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    additional N-terminal methionine encoded by this …

    Promoter

    T7 and endogenous

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJK175

    Plasmid

    #71132

    Purpose

    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli

    Depositor

    T. J. Kappock

    Article

    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    additional N-terminal methionine encoded by this …

    Promoter

    T7

    Available since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCellFree_G03 STAT1

    Plasmid

    #67116

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

27. pCellFree_G03 GTF2A1L

    Plasmid

    #67096

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2A1L (GTF2A1L Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pCellFree_G03 AKT1

    Plasmid

    #67102

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pCellFree_G03 GTF2H1

    Plasmid

    #67108

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2H1 (GTF2H1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

30. pCellFree_G03 TP53

    Plasmid

    #67078

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TP53 (TP53 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

31. pCellFree_G03 E-Cad

    Plasmid

    #67046

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    E-Cad (CDH1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pCellFree_G03 TCEB2

    Plasmid

    #67049

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TCEB2 (ELOB Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    M6R and Q115K

    Promoter

    T7

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

33. pDEST14-ARL6

    Plasmid

    #67380

    Purpose

    Expression of native hArl6 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL6 (ARL6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pDEST14-ARL8B

    Plasmid

    #67383

    Purpose

    Expression of native hArl8b in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8B (ARL8B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pDEST14-ARL14

    Plasmid

    #67385

    Purpose

    Expression of native hArl14 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL14 (ARL14 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    bp 172 C to T; aa L58F

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pDEST14-ARFRP1

    Plasmid

    #67387

    Purpose

    Expression of native hArfrp in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARFRP1 (ARFRP1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pDEST14-ARF1

    Plasmid

    #67369

    Purpose

    Expression of native hArf1 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF1 (ARF1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pDEST14-ARF5

    Plasmid

    #67372

    Purpose

    Expression of native hArf5 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF5 (ARF5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    bp 477 T to C; silent mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pDEST14-ARL1

    Plasmid

    #67374

    Purpose

    Expression of native hArl1 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL1 (ARL1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    bp 231 C to T; silent mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

40. pDEST14-ARL4

    Plasmid

    #67377

    Purpose

    Expression of native hArl4 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL4 (ARL4A Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    bp 105 A to T; silent mutation

    Promoter

    T7

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only