We narrowed to 63,680 results for: cloning
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Plasmid#17414PurposeGateway entry vector for Degron, N-term, frame ADepositorPublicationTypeEmpty backboneUseGateway CloningTagsFKBP12DD (L106P)ExpressionMammalianAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pEX-SP-CFP-STIM1(23-685)
Plasmid#18858DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStromal interaction molecule 1 (STIM1 Human)
TagsCFPExpressionMammalianMutationCFP inserted between residues 1-23 (signal peptid…Available sinceOct. 15, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pB1H2wL-Prd
Plasmid#18040DepositorInsertPaired (prd Fly)
ExpressionBacterialMutationPaired is simply used as an insert between Kpn1 a…Available sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFI1
Plasmid#16168DepositorPublicationInsertGrowth factor independent 1 (GFI1 Human)
ExpressionMammalianAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
kiCAP-AAV-MyoAAV4A
Plasmid#224448PurposeRep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationRGDYNSL insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Flag-TurboID-NES_pLX304
Plasmid#202008Purposeexpresses TurboID in the mammalian cytosol, lentiviral vectorDepositorInsertFlag-TurboID-NES
UseLentiviralTagsFlagExpressionMammalianPromoterCMVAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAWP78-PVCpnf_pvc13-Ad5Knob
Plasmid#198289PurposeProduces PVCs retargeted with knob domain from hAd5 virusDepositorInsertPVCpnf_pvc13-Ad5Knob
ExpressionBacterialAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-EFS-SaABE8e-bGH-U6-sgRNA-BsmBI
Plasmid#189922PurposeAAV genome encoding SaABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installationDepositorInsertSaABE8e
UseAAVMutationSaCas9 D10APromoterEFSAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-PEmax
Plasmid#187893PurposeAll-in-one prime editor piggyBac transposon with PEmaxDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK
UseCRISPR and Synthetic Biology; Piggybac transposonTagsBPSV40 NLS and c-Myc NLSExpressionMammalianPromoterCAGAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
HIF-TRE-mStrawberry reporter
Plasmid#158681PurposeThis plasmid is a HIF1A pathway reporter. It has a 6 x HIF binding element promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
RAR-TRE-mStrawberry reporter
Plasmid#158683PurposeThis plasmid is a RAR pathway reporter. It has a Retinoic Acid Receptor Response Element promoter driving the expression of mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti-EML4-ALK variant 1
Plasmid#183828PurposeExpress EML4-ALK fusion variant 1 (E13;A20) in mammalian cellsDepositorUseLentiviralTagsThe fusion protein of EML4 exon 1-13 and ALK exon…ExpressionMammalianPromoterCMVAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-rtTA
Plasmid#175274PurposeAAV vector mediating neuron-specific expression of rtTADepositorHas serviceAAV1InsertReverse tetracycline-controlled transactivator (rtTA)
UseAAVPromoterSynapsinAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi)
Plasmid#175573PurposeInactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repressionDepositorTypeEmpty backboneUseCRISPRAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AiP11633 - pAAV-hsA2-AiE0140h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1633)
Plasmid#163503PurposeDirect-expressing SYFP2 AAV VirusDepositorInsertSYFP2
UseAAVPromoterMinRhoAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-S5E6-dTom-nlsdTom
Plasmid#135641PurposeAAV vector to drive the expression of dTomato in VIP cortical interneurons under the control of the E6 regulatory elementDepositorHas serviceAAV1InsertdTom-nlsdTom
UseAAVMutationNoneAvailable sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-NLS-dPspCas13b-3xsfGFP-NLS
Plasmid#132404Purposeoverexpression in human cellsDepositorInsertdPspCas13b
UseLentiviralExpressionMammalianMutationH133A; H1059AAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-EF1A-PSD95.FingR-eGFP-CCR5TC
Plasmid#125691PurposeGlobal labeling of excitatory synapses (green fluorescence)DepositorInsertPSD95.FingR-eGFP-CCR5TC
UseAAVExpressionMammalianPromoterEF1AAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-GGamma9-GFP2
Plasmid#140991PurposeEncodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGGamma9-GFP2 (GNGT2 Human)
TagsGFP2 and GSAG linkerExpressionMammalianPromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5/FRT/TO-GAlphai1-RLuc8
Plasmid#140973PurposeEncodes a G alpha subunit (GNAl1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGAlphai1-RLuc8 (GNAI1 Human)
UseSynthetic BiologyExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by