We narrowed to 6,266 results for: wus

1. pU-eCFP

   Plasmid

   #176557

   Purpose

   Expression of eCFP for auxotrophic selection in the absence of uracil

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PL752-OTX2-LA-p2a-EGFPnls-hGHpA-PGK-puroR-OTX2-RA

   Plasmid

   #191690

   Purpose

   For knocking p2a-EGFP into OTX2 locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   OTX2 homology arms and p2a-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PL752-LMX1A-LA-p2a-tdTomato-hGHpA-PGK-puroR-LMX1A-RA

   Plasmid

   #191683

   Purpose

   For knocking p2a-tdTomato into LMX1A locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   LMX1A homology arms and p2a-tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-hA3A-eBE-Y132D

   Plasmid

   #113429

   Purpose

   Expresses hA3A-eBE-Y132D in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-eBE-Y132D (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y132D

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU-mRuby2

   Plasmid

   #176549

   Purpose

   Expression of mRuby2 for auxotrophic selection in the absence of uracil

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   yomRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pH-mRuby2

   Plasmid

   #176547

   Purpose

   Expression of mRuby2 for auxotrophic selection in the absence of histidine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   yomRuby2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330A-Yap1-E1-#1

   Plasmid

   #171517

   Purpose

   deletion of a genomic locus in Yap1 gene

   Depositor

   Hans Schöler

   Article

   Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

   Insert

   Yap1 (Yap1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330A-Yap1-E2-#1

   Plasmid

   #171518

   Purpose

   deletion of a genomic locus in Yap1 gene

   Depositor

   Hans Schöler

   Article

   Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

   Insert

   Yap1 (Yap1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-EGFP barcode-10-SV40 polyA

   Plasmid

   #190874

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode10
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mEos3-caRac1

    Plasmid

    #92041

    Purpose

    Expresses mEos3 fusion with caRac1 (Q61L)

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Use

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Q61L (constitutively active)

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE2-TetOminiCMV-Klf4

    Plasmid

    #136613

    Purpose

    Dox-inducible lentiviral vector expressing mouse Klf4

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    Klf4 (Klf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe))

    Plasmid

    #105210

    Purpose

    Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 3 intron - see PMID: 29056342 for detailed information)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pINDUCER21-puro_TAOK1

    Plasmid

    #209777

    Purpose

    doxycycline-inducible expression of TAOK1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    TAOK1 (TAOK1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiV_Neo_SIK3_CR_T221A

    Plasmid

    #108107

    Purpose

    Lentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    SIK3 (SIK3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    change guanine 501 to cytosine (silent mutation),…

    Promoter

    EFS promoter

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. PA-DreN

    Plasmid

    #169707

    Purpose

    The optimized DreN246-nMag

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    Dre246-nMag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. PA-DreC

    Plasmid

    #169705

    Purpose

    The optimized pMag-Dre247

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    pMag-Dre247
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

    Plasmid

    #136381

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-TV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D570A

    Promoter

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR221 NLRP1 A66V

    Plasmid

    #166817

    Purpose

    Gateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Expression

    Mutation

    A66V

    Promoter

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. SLC9A3R1_PDZ_2

    Plasmid

    #103933

    Purpose

    Protein expression and purification of SLC9A3R1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    SLC9A3R2 (NHERF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL421_MLL_promoter

    Plasmid

    #118695

    Purpose

    Express MLL/KMT2A promoter for luciferase assay

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Insert

    MLL_promoter
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    MLL/KMT2A promoter

    Available Since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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