59,538 results

1. pGEX-6P1-hPXR-LBD

   Plasmid

   #67769

   Purpose

   Expression GST-hPXR-LBD in bacteria

   Depositor

   Sridhar Mani

   Article

   Li et al J Biol Chem. 2013 May 10;288(19):13655-6

   Insert

   human pregnane X receptor (PXR) ligand binding domain (NR1I2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains amino acids 107 to 434 of human PXR

   Promoter

   Tac

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF1431

   Plasmid

   #143271

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF219 (ZNF219 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-COSMC-KO

   Plasmid

   #80008

   Purpose

   gRNA to knock out expression of COSMC (C1GalT1C1) gene. The product of this gene is a chaperone aiding in synthesis of Core1 structures on O-linked glycans.

   Depositor

   Sriram Neelamegham

   Article

   Stolfa et al Sci Rep. 2016 Jul 26;6:30392. doi: 10.10

   Insert

   COSMC (C1GALT1C1 Human)
   

   Use

   CRISPR

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. R777-E125 Hs.MAPK1

   Plasmid

   #70409

   Purpose

   Gateway ORF clone of human MAPK1 [NM_002745.4] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Gateway entry clone

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-Ef1a-hMGT-PGK-puro

   Plasmid

   #172394

   Purpose

   Lentiviral expression vector for human direct cardiac reprogramming

   Depositor

   Li Qian

   Article

   Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

   Insert

   Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
   

   Use

   Lentiviral

   Available Since

   July 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. MAC-NRP1

   Plasmid

   #158384

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

   Insert

   NRP1 (NRP1 Human)
   

   Tags

   MAC-tag

   Expression

   Mammalian

   Available Since

   Aug. 25, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pcDNA3-GFP-IMP2-2

   Plasmid

   #42175

   Depositor

   Alexandra Kiemer

   Article

   Kessler et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   Insulin-like growth factor 2 mRNA binding protein 2-2 (IGF2BP2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5FRT/Flag-WDR5

   Plasmid

   #29759

   Depositor

   Joan Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   WDR5 (WDR5 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 29, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pBa.TfR.GFP

   Plasmid

   #45060

   Depositor

   Gary Banker, Marvin Bentley

   Article

   Burack et al Neuron. 2000 May;26(2):465-72.

   Insert

   TfR (TFRC Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Chicken Beta Actin

   Available Since

   Oct. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. alpha7nACHR_Nacho_mCherry

    Plasmid

    #182939

    Purpose

    alpha7 nACHR expression in mammalian cell lines

    Depositor

    Robert Brenner

    Article

    Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

    Insert

    NACH7 (CHRNA7 Human)
    

    Use

    Synthesized

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. hsgk1

    Plasmid

    #83432

    Purpose

    full length human sgk1 with Flag tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Renal Physiol. 2008 Nov;295

    Insert

    human Sgk1 (SGK1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. G1A

    Plasmid

    #24690

    Depositor

    Jeanne Lawrence

    Article

    Clemson et al J Cell Biol. 1996 Feb . 132(3):259-75.

    Insert

    human XIST (XIST Human)
    

    Use

    Plasmid cloning vector

    Mutation

    Genomic insert. Includes introns 5-8.

    Available Since

    Jan. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF0883

    Plasmid

    #141749

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HOXB7 (HOXB7 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. ALKBH5-dCas9

    Plasmid

    #134783

    Purpose

    Expression fusion protein ALKBH5-dCas9 in Mammalian cells

    Depositor

    Shu-Bing Qian

    Article

    Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

    Insert

    ALKBH5 and inactive dCas9
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pN3-MGA

    Plasmid

    #107715

    Purpose

    Expression plasmid for 3xFLAG-tagged MGA

    Depositor

    Guntram Suske

    Article

    Stielow et al PLoS Genet. 2018 Jan 30;14(1):e1007193.

    Insert

    MGA (MGA Human)
    

    Tags

    3xFlag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pT7-EGFP-C1-Hs4E-T_AF

    Plasmid

    #148687

    Purpose

    Mammalian Expression of Hs4E-T

    Depositor

    Elisa Izaurralde

    Article

    Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

    Insert

    Hs4E-T (EIF4ENIF1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEYFP-C1-Drp1

    Plasmid

    #45160

    Depositor

    Richard Youle

    Article

    Frank et al Dev Cell. 2001 Oct;1(4):515-25.

    Insert

    Drp1 (DNM1L Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVS1-TRE-SHH

    Plasmid

    #134350

    Purpose

    Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus

    Depositor

    Lorenz Studer

    Article

    Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.

    Insert

    SHH (SHH Human)
    

    Expression

    Mammalian

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

    Plasmid

    #103866

    Purpose

    dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. BFP-Sec61 beta

    Plasmid

    #49154

    Purpose

    expression of Sec61 beta fused to BFP, used as a general ER marker

    Depositor

    Gia Voeltz

    Article

    Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

    Insert

    Sec61 beta (SEC61B Human)
    

    Tags

    BFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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