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Showing: 7281 - 7320 of 14669 results

  5. pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40]

    Plasmid
    #124069
    Purpose
    CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony and Dm-yellow for linearization; (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Insert
    eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40
    Use
    CRISPR and Cre/Lox
    Expression
    Insect
    Promoter
    bhsp68
    Available Since
    July 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    124069-plasmid-map-sequence-id-240664

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  6. pCMV-T7-ABE8e-SpCas9-HF1-P2A-EGFP (LM422)

    Plasmid
    #197505
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-HF1(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-ABE8e-nSpCas9-HF1-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA and nSpCas9-HF1(D10A/N497…
    Promoter
    CMV and T7
    Available Since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    197505-plasmid-map-sequence-id-386783

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  8. KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pA

    Plasmid
    #121800
    Purpose
    pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven GFP-P2A-TETa
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    HA epitope tag-polyA + CMV::eGFP-P2A-TETa-polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121800-plasmid-map-sequence-id-237308

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  12. pBac[3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP]

    Plasmid
    #86456
    Purpose
    Lox responder construct with floxed (same orientation; 2xloxP, 2xlox2272) mCherry (cy) body marker and H2Av::EGFP (nu) for Cre dependent cell clone marking; (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Insert
    3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86456-plasmid-map-sequence-id-171156

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  14. pWPT-ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49228
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    EF1-short
    Available Since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49228-plasmid-map-sequence-id-73645

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  15. pBEST-p70a-UTR1-deGFP Y35TAG-6xHis-T500

    Plasmid
    #92225
    Purpose
    Expression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1
    Depositor
    Lital Alfonta
    Article
    Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021
    Insert
    deGFP Y35TAG
    Use
    Synthetic Biology; Cell free protein synthesis (c…
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Residue Y35 mutated into TAG (Amber) stop codon f…
    Promoter
    p70a
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    92225-plasmid-map-sequence-id-197052

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  18. pCru5-ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid
    #49219
    Purpose
    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Article
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Retroviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    Viral LTR (or CMV)
    Available Since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49219-plasmid-map-sequence-id-73627

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  21. pLVX-EF1_-EGFR- FCRL3-50aa-Chimera-puro

    Plasmid
    #248150
    Purpose
    Lentiviral vector encoding a chimeric protein composed of the transmembrane region of FCRL3 and its truncated cytoplasmic tail (aa 595-644; first 50 residues), fused to the extracellular domain of EGFR.
    Depositor
    Silvia Monticelli
    Article
    Bianchi et al J Exp Med (2026) 223 (1): e20242474.
    Insert
    EGFR-FCRL3 (FCRL3 Human)
    Use
    Lentiviral
    Promoter
    EF1_
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    248150-plasmid-map-sequence-id-495277

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  22. pLVX-EF1_-EGFR-FCRL3-93aa-Chimera-puro

    Plasmid
    #248149
    Purpose
    Lentiviral vector encoding a chimeric protein composed of the transmembrane region of FCRL3 and its truncated cytoplasmic tail (aa 595-687; first 93 residues), fused to the extracellular domain of EGFR.
    Depositor
    Silvia Monticelli
    Article
    Bianchi et al J Exp Med (2026) 223 (1): e20242474.
    Insert
    EGFR-FCRL3 (FCRL3 Human)
    Use
    Lentiviral
    Promoter
    EF1_
    Available Since
    Dec. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    248149-plasmid-map-sequence-id-495275

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  23. pLV-hU6-ZNF865-hUbC-dCas9-KRAB-eGFP

    Plasmid
    #241308
    Purpose
    3rd gen lenti vector encoding dCas9 (s. Pyogenes) fused with the KRAB and a 2A eGFP fluorescence marker. Includes guide for suppression of the ZNF865 gene
    Depositor
    Robby Bowles
    Insert
    ZNF865 sgRNA (ZNF865 Human)
    Use
    Lentiviral
    Available Since
    Dec. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    241308-plasmid-map-sequence-id-483070

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  24. pCMV-SpCas9-VRQR(+L1111R)-P2A-EGFP (LRF167)

    Plasmid
    #242673
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(L1111R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + L1111R mutations in SpCas9(L1111R/D1135V/G…
    Promoter
    CMV and T7
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242673-plasmid-map-sequence-id-494379

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  25. pCMV-SpCas9-VRQR(+A1322R)-P2A-EGFP (LRF59)

    Plasmid
    #242679
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A1322R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(A1322R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + A1322R mutations in SpCas9(D1135V/G1218R/A…
    Promoter
    CMV and T7
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242679-plasmid-map-sequence-id-494380

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  27. pAAV CMV-hTau-T2A-EGFP-WPRE3-BGHpA

    Plasmid
    #240284
    Purpose
    The plasmid will package wild-type human Tau (hMAPT) as a T2A fusion with EGFP from a CMV promoter
    Depositor
    Bradley Hyman, Andrew Steele
    Insert
    hMAPT (MAPT Human)
    Use
    AAV
    Tags
    T2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240284-plasmid-map-sequence-id-479124

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  28. pCMV-SpCas9-VRQR(+R221K)-P2A-EGFP (LRF95)

    Plasmid
    #242666
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(R221K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(R221K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + R221K mutations in SpCas9(R221K/D1135V/G12…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242666-plasmid-map-sequence-id-489088

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  29. pCMV-SpCas9-VRQR(+N394K)-P2A-EGFP (KAC956)

    Plasmid
    #242670
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N394K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(N394K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + N394K mutations in SpCas9(N394K/D1135V/G12…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242670-plasmid-map-sequence-id-488989

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  30. pCMV-SpCas9-VRQR(+D269A)-P2A-EGFP (LRF64)

    Plasmid
    #242668
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D269A)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(D269A)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + D269A mutations in SpCas9(D269A/D1135V/G12…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242668-plasmid-map-sequence-id-488987

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  31. pCMV-SpCas9-VRQR(+G366R)-P2A-EGFP (KAC1026)

    Plasmid
    #242669
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(G366R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(G366R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + G366R mutations in SpCas9(G366R/D1135V/G12…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242669-plasmid-map-sequence-id-488988

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  32. pCMV-SpCas9-VRQR(+N1286Q)-P2A-EGFP (LRF247)

    Plasmid
    #242677
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N1286Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(N1286Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + N1286Q mutations in SpCas9(D1135V/G1218R/N…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242677-plasmid-map-sequence-id-488986

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  33. pCMV-SpCas9-VRQR(+G1104K)-P2A-EGFP (LRF54)

    Plasmid
    #242671
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + G1104K mutations in SpCas9(G1104K/D1135V/G…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242671-plasmid-map-sequence-id-488983

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  34. pCMV-SpCas9-VRQR(+A61R)-P2A-EGFP (KAC1030)

    Plasmid
    #242665
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A61R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(A61R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + A61R mutations in SpCas9(A61R/D1135V/G1218…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242665-plasmid-map-sequence-id-488982

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  35. pCMV-SpCas9-VRQR(+L244Q)-P2A-EGFP (LRF96)

    Plasmid
    #242667
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L244Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(L244Q)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + L244Q mutations in SpCas9(L244Q/D1135V/G12…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242667-plasmid-map-sequence-id-488981

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  36. pCMV-SpCas9-VRQR(+N1317R)-P2A-EGFP (LRF53)

    Plasmid
    #242678
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(N1317R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + N1317R mutations in SpCas9(D1135V/G1218R/N…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242678-plasmid-map-sequence-id-488980

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  37. pCMV-SpCas9-VRQR(+A1285K)-P2A-EGFP (KAC1134)

    Plasmid
    #242676
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(A1285K)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + A1285K mutations in SpCas9(D1135V/G1218R/A…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242676-plasmid-map-sequence-id-488979

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  38. pCMV-SpCas9-VRQR(+L1245V)-P2A-EGFP (LRF49)

    Plasmid
    #242675
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(L1245V)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + L1245V mutations in SpCas9(D1135V/G1218R/L…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242675-plasmid-map-sequence-id-488976

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  39. pCMV-SpCas9-VRQR(+D1332R)-P2A-EGFP (LRF45)

    Plasmid
    #242681
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(D1332R)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + D1332R mutations in SpCas9(D1135V/G1218R/D…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242681-plasmid-map-sequence-id-488974

    Enlarge

  40. pCMV-SpCas9-VRQR(+L1111H)-P2A-EGFP (LRF269)

    Plasmid
    #242672
    Purpose
    CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-SpCas9-VRQR(L1111H)-BPNLS(SV40)-3xFLAG-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    VRQR + L1111H mutations in SpCas9(L1111H/D1135V/G…
    Promoter
    CMV and T7
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242672-plasmid-map-sequence-id-488973

    Enlarge

Showing: 7281 - 7320 of 14669 results