We narrowed to 4,056 results for: lenti crispr plasmid

1. pLKO5.sgRNA.EFS.tRFP

   Plasmid

   #57823

   Purpose

   3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   tagRFP
   

   Use

   CRISPR and Lentiviral

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHAGE-TO-dCas9-P2A-HSA

   Plasmid

   #121936

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   dCas9-P2A-HSA
   

   Use

   CRISPR and Lentiviral

   Tags

   HSA

   Expression

   Mammalian

   Promoter

   CMV-TO

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657

   Plasmid

   #212961

   Purpose

   Lentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

   Depositor

   Roland Rad

   Article

   Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

   Insert

   DR1_CasRx_backbone
   

   Use

   CRISPR and Lentiviral

   Tags

   TagRFP657

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LRG2.1-BFP

   Plasmid

   #120577

   Purpose

   Lentiviral plasmid to co-express a guide RNA and EBFP.

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   Lentiviral

   Available Since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pXPR_050

   Plasmid

   #96925

   Purpose

   lentiviral expression of gRNA scaffold

   Depositor

   John Doench, David Root

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TRRAP sgRNA3

   Plasmid

   #138184

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRRAP

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   TRRAP (TRRAP Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TRRAP sgRNA1

   Plasmid

   #138182

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRRAP

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   TRRAP (TRRAP Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TRRAP sgRNA2

   Plasmid

   #138183

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRRAP

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   TRRAP (TRRAP Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TLCV2

   Plasmid

   #87360

   Purpose

   LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Insert

   Cas9-2A-eGFP
   

   Use

   CRISPR and Lentiviral; Doxycycline inducible; egf…

   Tags

   Cas9-T2A-eGFP

   Expression

   Mammalian

   Promoter

   Tight TRE promoter

   Available Since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXPR_206

    Plasmid

    #96920

    Purpose

    for SaCas9 knockout, lentiviral expression of S. aureus Cas9 (SauCas9) and gRNA scaffold

    Depositor

    John Doench, David Root

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO5.sgRNA.EFS.PAC

    Plasmid

    #57825

    Purpose

    Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    PAC
    

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJRH051

    Plasmid

    #171625

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-BsmBI-Destination
    mNeon
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1-a and U6

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLX-sgRNA-BfuAI-2k

    Plasmid

    #112915

    Purpose

    Empty sgRNA expression plasmid

    Depositor

    Ren-Jang Lin

    Article

    Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMCB306

    Plasmid

    #89360

    Purpose

    sgRNA expression vector with GFP, puromycin resistance, BlpI+BstXI cloning sites

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKHH030

    Plasmid

    #89358

    Purpose

    hU6 sgRNA cloning vector for CDKO system

    Depositor

    Michael Bassik

    Article

    Han et al Nat Biotechnol. 2017 Mar 20. doi: 10.103

    Insert

    GFP, Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV hU6-gRNA(anti-sense) hUbC-VP64-dCas9-VP64-T2A-GFP

    Plasmid

    #66707

    Purpose

    expresses a single gRNA and VP64-dCas9-VP64

    Depositor

    Charles Gersbach

    Inserts

    hU6-gRNA expression cassette
    S.Pyogenes VP64-dCas9-VP64
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    VP64

    Mutation

    D10A and H840A

    Promoter

    hU6 and hUbC

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPUR-hU6-sgRNA-Sirius-8XMS2

    Plasmid

    #121942

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XMS2
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    human U6 promoter

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLH-sgRNA-Sirius-8XMS2

    Plasmid

    #121939

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XMS2
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    human U6 promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPUR-mU6-sgRNA-Sirius-8XPP7

    Plasmid

    #121943

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XPP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    mouse U6 promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLH-sgRNA-Sirius-8XPP7

    Plasmid

    #121940

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-8XPP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    human U6 promoter

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge