163,606 results

1. pTRE3G-Bi-β-chain

   Plasmid

   #133731

   Purpose

   Expresses the gePSI-β-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

   Depositor

   Erin Schuman

   Article

   Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

   Inserts

   b-Chain_gePSI
   ZsGreen1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Only one part of the gePSI-system (startcodon + a…

   Promoter

   pTRE3G-Bi

   Available Since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SNX9-pmCherryC1

   Plasmid

   #27678

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   SNX9 (Snx9 Mouse)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-Tet-OsTIR1(WT)-V5

   Plasmid

   #158663

   Purpose

   Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   Tet-OsTIR1(WT) AAVS1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.10-Claudin-1

   Plasmid

   #46387

   Purpose

   Claudin-1 promoter reporter assay

   Depositor

   David Mu

   Article

   Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

   Insert

   CLDN1 Promoter (CLDN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   CLDN1 promoter

   Available Since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pCDNA5FRT/TO_HA-mAID-nanobody

   Plasmid

   #117713

   Purpose

   Expression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)

   Depositor

   Joerg Mansfeld

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Insert

   mAID-nanobody
   

   Use

   Tags

   triple HA

   Expression

   Mammalian

   Mutation

   codon optimized mAID for murine expression

   Promoter

   CMV

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-CMV-human MYCN-EF1-mRFP

   Plasmid

   #193708

   Purpose

   Lentiviral expression of human MYCN

   Depositor

   Martine Roussel

   Insert

   MYCN (MYCN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSL3713 (pTnsC_Pse)

   Plasmid

   #200900

   Purpose

   Expresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseTnsC
   

   Use

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-Solo-mStayGold-CANX

   Plasmid

   #227280

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   CANX Homology Arms flanking a mStayGold Tag (CANX Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

   Plasmid

   #160736

   Purpose

   Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. LBR pEGFP-N2 (646)

    Plasmid

    #61996

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    LBR (LBR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. BcLOV4-mammalian-recoded_mCherry_pcDNA3.1

    Plasmid

    #114595

    Purpose

    Encodes mCherry-tagged BcLOV4 for expression in mammalian cells

    Depositor

    Brian Chow

    Article

    Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

    Insert

    BcLOV4
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDV05.2_pEF1a-iRFP720_secreted

    Plasmid

    #200409

    Purpose

    Expresses a secreted version of iRFP720 constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (secreted)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-HaloAKAR2.2-T2A-EGFP

    Plasmid

    #216490

    Purpose

    Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.2
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMPRAdonor2

    Plasmid

    #49353

    Purpose

    minP-luc2 donor plasmid for MPRA library construction

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Insert

    luc2
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    minP

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNUT N6His hTFNG

    Plasmid

    #67240

    Purpose

    Expresses N-His tagged nonglycosylated human serum transferrin in mammalian cells

    Depositor

    Anne Mason, Ross MacGillivray

    Article

    Mason et al Biochemistry. 2002 Jul 30;41(30):9448-54

    Insert

    human serum transferrin (TF Human)
    

    Use

    Tags

    N-terminal signal peptide, 4 aa link, 6 His, Fact…

    Expression

    Mammalian

    Mutation

    Asn413 Asp, Asn611Asp

    Promoter

    SV40

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR_Gal4UAS_Blinatumomab_PGK_mCherry

    Plasmid

    #85437

    Purpose

    [Edit] Lentiviral vector for Gal4 inducible Blinatumomab and constitutive mCherry expression

    Depositor

    Wendell Lim

    Article

    Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

    Insert

    Blinatumomab
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-EFS-LSL-nlsGFP-shRNA

    Plasmid

    #175584

    Purpose

    Conditional express shRNA with control of LoxP

    Depositor

    Christian Wolfrum

    Article

    Sun et al Nature. 2020 Nov;587(7832):98-102. doi:

    Type

    Empty backbone

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pN1-RnKIF5C(1-560,Δ6)-Halo-Flag

    Plasmid

    #188072

    Purpose

    Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags. The delta6 mutation removes the first 6 amino acids.

    Depositor

    Kristen Verhey

    Article

    Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

    Insert

    rat KIF5C (Kif5c Rat)
    

    Use

    Tags

    Flag and Halo

    Expression

    Mammalian

    Mutation

    aa1-560, deletion of aa2-6

    Promoter

    CMV

    Available Since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL3402 (pTnsABf_Pse)

    Plasmid

    #200901

    Purpose

    Expresses human codon-optimized Pseudoalteromonas TnsA-TnsB fusion from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseTnsA-TnsB
    

    Use

    Tags

    Internal NLS between TnsA-TnsB fusion

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-flex-taCasp3-TEVp

    Plasmid

    #45580

    Depositor

    Nirao Shah, Jim Wells

    Article

    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

    Has Service

    AAV1 and AAV5

    Inserts

    Caspase 3 (CASP3 Human, Synthetic)
    Tobacco Etch Virus protease
    

    Use

    AAV; Adeno associated viral vector

    Tags

    Expression

    Mutation

    Linker replaced with a TEV protease cleavage site

    Promoter

    EF1a

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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