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pIM1773-PRHIII-sfGFP-pNW33N

Plasmid

#52217

Purpose

Constitutive sfGFP expression plasmid for use in Geobacillus and E. coli

Depositor

Ichiro Matsumura

Article

Blanchard et al Appl Microbiol Biotechnol. 2014 May 2.

Insert

PRHIII-sfGFP

Expression

Bacterial

Promoter

PRHIII

Available Since

May 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAC1801_pmax-dCasRx

Plasmid

#118634

Purpose

Transient transfection; Expresses dCasRx; CAGGS promoter

Depositor

Albert Cheng

Article

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

dCasRx

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A

Promoter

CAGGS

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pYY10-2µ-TP901

Plasmid

#235771

Purpose

2µ plasmid for constitutive expression of TP901 under RNR2 promoter.

Depositor

Chang Liu

Article

Pisera et al ACS Synth Biol. 2025 Apr 18;14(4):1002-1

Insert

TP901 integrase

Expression

Yeast

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-Brainbow-2.1 R

Plasmid

#18723

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

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CMV-Brainbow-1.1 M

Plasmid

#18722

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pTRC-APEX2

Plasmid

#72558

Purpose

Expression of cytosolic APEX2 in bacteria

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2

Tags

His6

Expression

Bacterial

Mutation

A134P relative to APEX

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pPTK021-7-PARS

Plasmid

#84990

Purpose

Type 7, Pichia autonomously replicating sequence

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Pichia autonomously replicating sequence

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5-BALF5

Plasmid

#72630

Purpose

Mammalian expression vector for Epstein-Barr virus DNA polymerase

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BALF5

Expression

Mammalian

Mutation

G249S

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
pMOD3-mCardinal-nat

Plasmid

#120333

Purpose

Bacterial mCardinal promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mCardinal

Expression

Bacterial

Promoter

(None)

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEERM4 Cm

Plasmid

#64026

Purpose

Plasmid for integration into neutral site 2 of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PnrsB

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pEERM3 Km

Plasmid

#64025

Purpose

Plasmid for integration into neutral site of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PnrsB

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-Rev(NL4.3)

Plasmid

#115776

Purpose

pCMV-RevNL4.3 allows the expression of Rev protein.

Depositor

Damian Purcell

Article

Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

Insert

HIV-1 Rev protein (NL4.3)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pEERM1 Km

Plasmid

#64024

Purpose

Plasmid for integration into psbA2 site of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PpsbA2

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pKJ0463

Plasmid

#228715

Purpose

Bacterial expression of epT7 RNAP; for protein production and purification

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Science 0, eadr6006

Insert

Sumo_epT7

Expression

Bacterial

Mutation

T3M/G47A/E643G

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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mVenus-N1

Plasmid

#54640

Purpose

Localization: N1 Cloning Vector, Excitation: 515, Emission: 528

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Tags

mVenus

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pWS3805

Plasmid

#185746

Purpose

Sub-array spacer 1/4

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Insert

CRISPRai sub-array spacer 1/4

Use

CRISPR

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-rtTA

Plasmid

#185562

Purpose

Ubiquitous expression of reverse tetracycline transactivator for TetON system

Depositor

Connie Cepko

Article

Amamoto et al Elife. 2022 Mar 22;11. pii: 76389. doi:

Insert

rtTA

Expression

Mammalian

Promoter

CAG

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pZ8-T_dCas9

Plasmid

#74062

Purpose

pZ8-1 plasmid carrying dcas9, driven by the IPTG-inducible Ptac promoter, KanR

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Insert

dcas9

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

ptac

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGL0_65 [StayGold]

Plasmid

#198976

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

StayGold

Use

Synthetic Biology

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pEERM6 Km

Plasmid

#64027

Purpose

Plasmid for integration into sqs site of Synechocystis 6803

Depositor

Pia Lindberg

Article

Englund et al ACS Synth Biol. 2015 Jul 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

PnrsB

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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