We narrowed to 70,738 results for: LAS

1. pSBbi-Pur-AktAR2-D

   Plasmid

   #125196

   Purpose

   SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporter

   Depositor

   Eric Davis

   Insert

   AktAR2-D
   

   Use

   Transposon

   Tags

   6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…

   Expression

   Mammalian

   Mutation

   FoxO1 Thr-24 changed to Val rendering this inacti…

   Promoter

   EF1a/RPBSA

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZNF696.1.0-gDNA

   Plasmid

   #113773

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF696

   Depositor

   Kevin White

   Insert

   ZNF696 (ZNF696 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZNF561.1.0-gDNA

   Plasmid

   #113768

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF561

   Depositor

   Kevin White

   Insert

   ZNF561 (ZNF561 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZNF700.1.0-gDNA

   Plasmid

   #112481

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF700

   Depositor

   Kevin White

   Insert

   ZNF700 (ZNF700 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZNF169.2.0-gDNA

   Plasmid

   #112472

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF169

   Depositor

   Kevin White

   Insert

   ZNF169 (ZNF169 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZNF227.1.0-gDNA

   Plasmid

   #112464

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF227

   Depositor

   Kevin White

   Insert

   ZNF227 (ZNF227 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTX1.1.0-gDNA

   Plasmid

   #112460

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor OTX1

   Depositor

   Kevin White

   Insert

   OTX1 (OTX1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZNF766.1.0-gDNA

   Plasmid

   #112448

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF766

   Depositor

   Kevin White

   Insert

   ZNF766 (ZNF766 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZNF586.1.0-gDNA

   Plasmid

   #112420

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF586

   Depositor

   Kevin White

   Insert

   ZNF586 (ZNF586 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZNF565.1.0-gDNA

    Plasmid

    #112411

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF565

    Depositor

    Kevin White

    Insert

    ZNF565 (ZNF565 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCWXPGR-pTF-EpoRtm

    Plasmid

    #114286

    Purpose

    TET-inducible expression of Erythropoietin receptor without cytoplasmic tail

    Depositor

    Patrick Salmon

    Insert

    Erythropoietin Receptor (Epor Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    deletion of the cytoplasmic domain

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. 4MYI

    Plasmid

    #60103

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PVX_084705:MAC04G-C11:C228845
    

    Use

    Baculovirus expression

    Tags

    6x His and TEV cleavage site

    Expression

    Mutation

    See comments

    Promoter

    polyhedrin

    Available since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pLV6-Bmal-luc

    Plasmid

    #68833

    Purpose

    Expression of Bmal1-luc in mammalian cells

    Depositor

    Steven Brown

    Insert

    Bmal1 regulatory sequences (Bmal1 Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFGC-pcoCas9

    Plasmid

    #52256

    Purpose

    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

    Depositor

    Jen Sheen

    Insert

    35SPPDK:pcoCas9:NOS cassette
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    constitutive 35SPPDK promoter

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti-UCOE-dCas9-BFP-Zim3-KRAB

    Plasmid

    #196716

    Purpose

    CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-TagBFP-KRAB(ZIM3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG/HA-hBIG2

    Plasmid

    #226254

    Purpose

    Expresses N-terminally HA-tagged hBIG2 in mammalian cells

    Depositor

    Hye-Won Shin

    Insert

    BIG2 (ARFGEF2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCellFree_KA1_SARS-COV-2_ORF7a

    Plasmid

    #169393

    Purpose

    Cell-free/mammalian expression vector for SARS-COV-2 virus ORF7a with N-term eGFP

    Depositor

    Kirill Alexandrov

    Insert

    SARS-COV-2 _ORF7a (ORF7a SARS-Cov2)
    

    Use

    Cell-free expression vector

    Tags

    His and eGFP

    Expression

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    T7- CMV/CAG

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-FLEX-SaCas9-U6-sgOprk1

    Plasmid

    #159903

    Purpose

    Mutagenesis of Oprk1

    Depositor

    Larry Zweifel

    Insert

    Oprk1 (Oprk1 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCB265

    Plasmid

    #69748

    Purpose

    Retroviral expression of HURP

    Depositor

    Iain Cheeseman

    Insert

    HURP (DLGAP5 Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

21. pSPneoHHgRNAH

    Plasmid

    #63557

    Purpose

    Expresses gRNA in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

    Insert

    guide sequence
    

    Use

    Leishmania expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV asyn S129A

    Plasmid

    #36066

    Depositor

    Patrick Aebischer, Hilal Lashuel

    Insert

    alpha-synuclein S129A (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S129A

    Promoter

    CMV

    Available since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

23. pENTR1A-Huwe1 C4341S

    Plasmid

    #37432

    Depositor

    Jean Cook

    Insert

    Huwe1 (HUWE1 Human)
    

    Use

    Gateway entry

    Tags

    Expression

    Mutation

    C4341S

    Promoter

    Available since

    Sept. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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24. pAAV asyn S129D

    Plasmid

    #36069

    Depositor

    Patrick Aebischer, Hilal Lashuel

    Insert

    alpha-synuclein S129D (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S129D

    Promoter

    CMV

    Available since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

25. pJPC13

    Plasmid

    #80773

    Purpose

    Cloning vector compatible with ColE1 plasmids with medium copy number

    Depositor

    Gregory Phillips

    Article

    Peterson et al Plasmid. 2008 May;59(3):193-201. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pSPneogRNAH

    Plasmid

    #63556

    Purpose

    Expresses gRNA in Leishmania

    Depositor

    Greg Matlashewski

    Article

    Zhang et al MBio. 2015 Jul 21;6(4). pii: e00861-15.

    Insert

    guide sequence
    

    Use

    Leishmania expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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27. pLNHA-C1-HsPAN2_M

    Plasmid

    #146941

    Purpose

    Mammalian Expression of HsPAN2

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsPAN2 (PAN2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pMTF1.1.0-gDNA

    Plasmid

    #113797

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor MTF1

    Depositor

    Kevin White

    Insert

    MTF1 (MTF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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29. pJPK13

    Plasmid

    #80775

    Purpose

    Cloning vector compatible with ColE1 plasmids with high copy number

    Depositor

    Gregory Phillips

    Article

    Peterson et al Plasmid. 2008 May;59(3):193-201. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. p404MET25

    Plasmid

    #17420

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    MET25 promoter + polylinker + CYC1 terminator (MET17 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    MET25 promoter is between SacI and XbaI restricti…

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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