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pCMV 3xFLAG-GABARAP G116

Plasmid

#123099

Purpose

Expresses 3xFLAG-GABARAP G116 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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p3E-CDC42-DN

Plasmid

#109584

Purpose

Multisite gateway vector for 3' tagging with dominant negative CDC42

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

CDC42 (cdc42 Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-pegRNA_KCNA1-EGFP

Plasmid

#159787

Purpose

S. pyogenes pegRNA for C to A mutation at the KCNA1 site of human cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA targeting KCNA1 gene (KCNA1 Human)

Expression

Mammalian

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pH3R-P2A-mCherry-N1

Plasmid

#84332

Purpose

Co-expresses (untagged) Histamine-3 receptor and mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H3R-P2A-mCherry (HRH3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pPBpuro-ErbB1(DEL1011)

Plasmid

#197366

Purpose

Encoding human ErbB1 mutant.

Depositor

Michiyuki Matsuda

Article

Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

Insert

EGFR mutant. (EGFR Human)

Expression

Mammalian

Mutation

deleted amino acids 1011-1186

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCR2.1-promoter-mIL-6

Plasmid

#109296

Purpose

IL-6 promoter reporter construct

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

murine IL-6 promoter (Il6 Mouse)

Expression

Mammalian

Mutation

CMV promoter in pEGFP-N1 replaced with murine IL-…

Promoter

murine IL-6 promoter

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2-Cx43-siResist

Plasmid

#49852

Purpose

Encodes human connexin 43 with wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Contains several wobble mutations to confer resis…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
YFP-Stim1-ΔCTD

Plasmid

#186621

Purpose

Encodes Stim1 with C-terminal domain deletion fused to YFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Stromal Interaction Molecule 1 (STIM1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Deletion of C-terminal domain 601 to 685

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR Del2

Plasmid

#32065

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Delete S752-I759

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2b-K660N

Plasmid

#45707

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

K660N

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pLPCX-Cx43-FS-IRES-GFP

Plasmid

#65438

Purpose

Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

frameshift (FS) mutation 35delA

Promoter

CMV

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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F11-R pEBio

Plasmid

#61486

Purpose

Expresses full-length F11R (JAM1) ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag and biotinylation sequence

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

F11R (F11R Human)

Tags

enzymatic biotinylation sequence and rat CD4 d3+4

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pBa.FRB-3myc-GS-KIF3A tail 419-702

Plasmid

#134614

Purpose

Mammalian expression of human KIF3A 419-702

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF3A (KIF3A Human)

Tags

FRB, 3myc

Expression

Mammalian

Mutation

WT with deletion of the 418 N-terminal residues.

Promoter

Chicken Beta actin

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-sACt-mCherry

Plasmid

#138216

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; plasma membrane targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

Lyn-sACt-mCherry (Adcy10 Rat)

Tags

N-terminal targeting sequence from Lyn kinase and…

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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CC2

Plasmid

#21207

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek1 (Esr1, MAP2K1 Mouse, Human)

Use

Subclone vector

Mutation

Mek1 R4F fused to ER*, constitutive active

Available Since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
pWZLblasti-HA-MEK1

Plasmid

#53161

Purpose

Expresses HA tagged human MEK1 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only
sgRNA1_IL1RN

Plasmid

#64140

Purpose

Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

Depositor

Moritoshi Sato

Article

Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

Insert

IL1RN (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR3C1_Hormone-recep

Plasmid

#110018

Purpose

Protein expression and purification of NR3C1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR3C1_Hormone-recep (NR3C1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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DN9

Plasmid

#21209

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek1 (Esr1, MAP2K1 Mouse, Human)

Use

Subclone vector

Mutation

Mek1 K97M fused to ER*, catalytically inactive

Available Since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
mRuby2-Actin-C-18

Plasmid

#55889

Purpose

Localization: Actin, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

Actin (ACTB Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits