We narrowed to 24,603 results for: Nes

1. Nano-lantern(Ca2+)_300/pcDNA3

   Plasmid

   #51983

   Purpose

   mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM_E104Q-3GS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   April 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDsRed (GB0100)

   Plasmid

   #68202

   Purpose

   Provides the CDS of DsRed as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   DsRed coding region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLIK 3xFLAG-MTUS1 zeo

   Plasmid

   #192323

   Purpose

   Lentiviral expression vector for an inducible 3xFLAG-MTUS1

   Depositor

   Kevin Janes

   Article

   Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

   Insert

   MTUS1 (MTUS1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMaCTag-Z09

   Plasmid

   #120052

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mVenus

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mVenus
   

   Use

   Pcr template

   Tags

   mVenus

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX330A_D10A-1x6

   Plasmid

   #58776

   Purpose

   Expresses Cas9 nickase and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

   Insert

   humanized S. pyogenes Cas9 (D10A) nickase
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D10A nickase-converting mutation

   Promoter

   CBh

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMaCTag-Z17

   Plasmid

   #120060

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mEos4b

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mEos4b
   

   Use

   Pcr template

   Tags

   mEos4b

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMaCTag-P14

   Plasmid

   #120025

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mNeptune2.5

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mNeptune2.5
   

   Use

   Pcr template

   Tags

   mNeptune2.5

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT7-Venus-Emi1 (914)

   Plasmid

   #39854

   Depositor

   Jonathon Pines

   Article

   Di Fiore et al J Cell Biol. 2007 May 7;177(3):425-37.

   Insert

   Emi1 (FBXO5 Human)
   

   Use

   In vitro transcription

   Tags

   Venus

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. p3XP3-WhiteA2

   Plasmid

   #165875

   Purpose

   Basic transgenesis 3xP3 white CDS physical eye marker cassette in Alpha2, lacks attB site

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Eye Marker Cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMaCTag-P15

    Plasmid

    #120026

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    miRFP670
    

    Use

    Pcr template

    Tags

    miRFP670

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMaCTag-Z33

    Plasmid

    #120050

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with FLAG

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    FLAG
    

    Use

    Pcr template

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CeNL(Ca2+)_1.2μ-pcDNA3

    Plasmid

    #111926

    Purpose

    Cyan color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_1.2μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTRF.1-mCMV-udsVenus

    Plasmid

    #58518

    Purpose

    Empty lentiviral expression vector for ultradestabilized Venus that indicates the activity of a transcriptional element of interest

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    None
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Nano-lantern(Ca2+)_600/pcDNA3

    Plasmid

    #51982

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=600nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-2G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pMaCTag-P24

    Plasmid

    #120035

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    TAP
    

    Use

    Pcr template

    Tags

    TAP

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMaCTag-P10

    Plasmid

    #120021

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKOk

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mKOk
    

    Use

    Pcr template

    Tags

    mKOk

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. OeNL(Ca2+)_18μ-pcDNA3

    Plasmid

    #111928

    Purpose

    Orange color bright luminescent calcium indicator with intermediate affinity

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    OeNL(Ca2+)_18μ
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMaCTag-P18

    Plasmid

    #120029

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mKeima8.5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mKeima8.5
    

    Use

    Pcr template

    Tags

    mKeima8.5

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCLC-mEos2

    Plasmid

    #38010

    Depositor

    Xiaowei Zhuang

    Article

    Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    mEos2

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR221-Firefly

    Plasmid

    #79495

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Firefly Luciferase CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    Firefly Luciferase
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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