We narrowed to 87,489 results for: lias
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pTE4398
Plasmid#74042PurposeExpresses human codon-optimized LbCpf1 and Lb crRNA.DepositorInsertsLb crRNA
LbCpf1
UseCRISPRTags3xHA and NLSExpressionMammalianMutationPromoterCMV and human U6Available sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
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TOM20MTS-mCherry-EGFP-Tet-On
Plasmid#109016PurposeTet-On construct to induce expression of tandem eGFP-mCherry tagged TOM20MTS (mitochondriaDepositorInsertTOM20MTS (MTS: mitochondria targeting signal) (TOMM20 Human)
UseLentiviralTagsmCherry-eGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLX303-ABI1-dCas9
Plasmid#154474PurposeExpresses ABI1 fused to the N terminus of dCas9DepositorInsertABI1 (ABI1 Mustard Weed)
UseLentiviralTagsExpressionMammalianMutationIncludes ABI1 aa 126-423, D143APromoterCMVAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
P2RY11-DuET
Plasmid#213364PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertP2RY11 (P2RY11 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330a dCas9-KRAB
Plasmid#92361PurposeModified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.DepositorInsertdCas9-KRAB
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC3-hGli3
Plasmid#65435PurposeExpresses human Gli3 protein in mammalian cellsDepositorInsertGli3 (GLI3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
lenti CAG-FLAG-dCas9-VPR
Plasmid#114197PurposeLentivirus compatible dCas9-VPR construct driven by the CAG promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG dCas9-KRAB-2A-EGFP
Plasmid#92396PurposeCAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.DepositorInsertdCas9_KRAB_2A_EGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-AP-Sstr3-GFP-DEST
Plasmid#49098PurposeExpressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell linesDepositorInsertSstr3 (Sstr3 Mouse)
UseTagsGFP and acceptor peptide (AP)ExpressionMammalianMutationPromoterT7Available sinceNov. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcU6_1 sgRNA
Plasmid#92395PurposeChicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.DepositorInsertchick U6.1 promoter and gRNA cloning cassette
UseCRISPRTagsExpressionMammalianMutationPromoterchick U6.1Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
OPN3-DuET
Plasmid#213357PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertOPN3 (OPN3 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2
Plasmid#215327PurposeLentiviral mammalian vector with firefly luciferase reporter for MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV promoter.DepositorInsertMUC5AC Promoter
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterMUC5ACAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2
Plasmid#215328PurposeLentiviral mammalian vector with firefly luciferase reporter for FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV promoter.DepositorInsertFOXJ1 Promoter
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterFOXJ1Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet-I3
Plasmid#189755PurposeProduces an mTurquoise-antiFRET-mScarlet-I3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet-I3 FPs for ratiometric analysisDepositorInsertmTurquoise2_spatial-linker-P2A_mScarlet-I3
UseTags6xHisExpressionBacterial and MammalianMutationPromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-AP-Smo-YFP-DEST
Plasmid#49099PurposeExpressing AP-Smo-YFP in mammalian cells, can be used for establishing Flp-In stable cell linesDepositorInsertSmo (Smo Mouse)
UseTagsYFP and acceptor peptide (AP)ExpressionMammalianMutationPromoterT7Available sinceNov. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
GST-Rab8a-wt
Plasmid#86078PurposeA bacterial expression plasmid encoding Rab8a wild type with N-terminus GST.DepositorInserthRab8A (RAB8A Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTone-gata2aECE-Kaede
Plasmid#132976Purposegata2a endothelial enhancer (x6) and basal promoter driving kaede in pTol1 backboneDepositorInsertsKaede
gata2a endothelial enhancer (x6) and a carp b-actin basal promoter
UseUnspecified; Transposon-mediatedTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1 MOSPD3
Plasmid#226405PurposeExpression of human MOSPD3 fused to EGFP in mammalian cellsDepositorInsertMOSPD3 (MOSPD3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only
