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pMON-HsNMT1

Plasmid

#67475

Purpose

Expression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteria

Depositor

Richard Kahn

Article

Van Valkenburgh et al Methods Enzymol. 2002;344:186-93.

Insert

HsNMT1 (NMT1 Human)

Expression

Bacterial

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pTD-NStrepHis

Plasmid

#45936

Purpose

use to create N-terminal Strep-tagII fusion proteins or dual tagged fusion with N-terminal Strep-tagII and C-terminal 6x His-tag

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Type

Empty backbone

Tags

6xHis and Strep II

Expression

Bacterial

Promoter

Trc (lactose/IPTG inducible)

Available Since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pST-2K

Plasmid

#44564

Purpose

for ectopic constitutive expression of two mycobacterial proteins in one vector

Depositor

Vinay Nandicoori

Article

Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

Type

Empty backbone

Use

E.coli-mycobacteria shuttle vector

Tags

Flag tag and His tag

Expression

Bacterial

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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XE107 XPKC alpha-GFP-CS2+

Plasmid

#16743

Depositor

Randall Moon

Article

Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

Insert

PKC-alpha (prkca Frog)

Use

Xenopus expression

Tags

GFP

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
Clomeleon

Plasmid

#203723

Purpose

A genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration

Depositor

George Augustine

Article

Kuner et al Neuron. 2000 Sep;27(3):447-59. doi: 10.1

Insert

cyan fluorescent protein linked yellow florescent protein

Expression

Bacterial and Mammalian

Mutation

Cyan Fluorescent Protein, Topaz Fluorescent Prote…

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pRS41K-mScarlet-I-mNeonGreen (pKBJ010)

Plasmid

#173461

Purpose

C-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-I-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJL017

Plasmid

#53532

Purpose

This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

Depositor

James Fleckenstein

Article

Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

Insert

etpBA

Tags

6His and myc

Expression

Bacterial

Promoter

pBAD

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pUC19/Pd-mChe

Plasmid

#183172

Purpose

Construct for transient expression of plasmodesmata-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

Pd-mCherry

Tags

AtPDCB1, A. thaliana PLASMODESMATA CALLOSE-BINDIN…

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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EGFP-MCPIP1

Plasmid

#127460

Purpose

Expressing EGFP-MCPIP1 fusion protein in mammalian cells

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

Plasmid

#199346

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19/CF-mChe

Plasmid

#183166

Purpose

Construct for transient expression of cytoplasmic foci-targeted mCherry in plants.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

CF-mCherry

Tags

AtTZF1, A. thaliana TANDEM ZINC FINGER PROTEIN 1

Expression

Plant

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMGS58

Plasmid

#135311

Purpose

A HygR-P2A-AID cassette to tag the protein of interest with auxin-inducible degron at the n-terminal

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

HygR-P2A-AID

Use

N terminal tagging plasmid

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pK-myc-Par6C

Plasmid

#15474

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Insert

Partitioning-defective protein 6C (PARD6A Human)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-Neo-LUC

Plasmid

#214368

Purpose

Expression of the firefly luciferase Protein

Depositor

Bertrand Collet

Article

Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

Insert

LUC

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3 T7 Jip4

Plasmid

#58925

Purpose

JIP4 serves as an activator of the p38 mitogen-activated protein (MAP) kinase pathway by a mechanism that requires the MAP kinase kinases MKK3 and MKK6

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2005 Apr;25(7):2733-43.

Insert

Mapk8ip4 (Spag9 Mouse)

Tags

T7

Expression

Mammalian

Available Since

Sept. 15, 2014

Availability

Academic Institutions and Nonprofits only
pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

Plasmid

#199356

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTE5400_pMBP_CDS dropout vector

Plasmid

#221189

Purpose

Expression vector for N-terminally His-tagged maltose binding protein (10xHis-MBP) fused to a protein of interest

Depositor

Tobias Erb

Article

Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

Type

Empty backbone

Tags

10x His-tag + E. coli maltose binding protein (MB…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR-PCBP1

Plasmid

#16177

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

Poly(rC) binding protein 1 (PCBP1 Human)

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
MAC-N-GFP

Plasmid

#172974

Purpose

MAC-tagged GFP expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

GFP

Tags

MAC-tag

Expression

Bacterial and Mammalian

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET12a-AL-09 FL

Plasmid

#47081

Depositor

Marina Ramirez-Alvarado

Article

McLaughlin et al Protein Sci. 2006 Jul;15(7):1710-22. Epu

Insert

AL-09 (IGKV3-20 Human)

Tags

ompT

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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