We narrowed to 17,015 results for: por

1. Myog'-2A-mCherryNLS-PGK-Purodtk

   Plasmid

   #69547

   Purpose

   Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

   Depositor

   Charles Gersbach

   Inserts

   2A-mCherry-NLS
   Myogenin 5' homology arm (Myog Mouse)
   Myogenin 3' homology arm (Myog Mouse)
   Puro-delta-tk
   

   Use

   Mouse Targeting

   Tags

   2x nuclear localization signal and PVT1-2A "…

   Mutation

   Silent V205V mutation in myogenin gene to prevent…

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-Blast

   Plasmid

   #205548

   Purpose

   Express EPN11-MCP-p6-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN11-MCP-p6
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. A3F-Cas9n-UGI

   Plasmid

   #119138

   Purpose

   Base editor made from APOBEC3F

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3F-Cas9 nickase-UGI
   

   Use

   CRISPR

   Mutation

   None

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFH2.96_PB-EPN1-T2A-eGFP-PGK-Blast

   Plasmid

   #205541

   Purpose

   Express EPN1-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN1
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFH2.100_PB-EPN11-T2A-eGFP-PGK-Blast

   Plasmid

   #205545

   Purpose

   Express EPN11-T2A-eGFP and blasticidin resistance marker

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   EPN11
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLD-puro-PGKnVA

   Plasmid

   #24589

   Depositor

   Jason Moffat

   Article

   Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

   Insert

   Gateway(TM) cassette
   

   Use

   Lentiviral

   Tags

   VA

   Expression

   Mammalian

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pBSU6-shTim23/CMV-eGFP

   Plasmid

   #89795

   Purpose

   Expresses shRNA against TIM23 with a separate promoter for expression of GFP.

   Depositor

   Robert Friedlander

   Article

   Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

   Insert

   shRNA Tim23 (Timm23 Mouse)
   

   Tags

   eGFP under separate promoter

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. A3C-Cas9n-UGI

   Plasmid

   #119136

   Purpose

   Base editor made from APOBEC3C

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   APOBEC3C-Cas9 nickase-UGI
   

   Use

   CRISPR

   Mutation

   None

   Promoter

   CMV

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pfTRAP

   Plasmid

   #50048

   Purpose

   Expresses plasmodium falciparum TRAP adhesion domains (26-299) in mammalian cells

   Depositor

   Timothy Springer

   Article

   Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

   Insert

   plamodium falciparum TRAP (TRAP Synthetic)
   

   Tags

   6Xhis tag

   Expression

   Mammalian

   Mutation

   C55G, N132S

   Promoter

   CMV

   Available Since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pfTRAP(VWA)

    Plasmid

    #50049

    Purpose

    Expresses plasmodium falciparum TRAP VWA domain (41-240) in mammalian cells

    Depositor

    Timothy Springer

    Article

    Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

    Insert

    pfTRAP(vwa) (TRAP Synthetic)
    

    Tags

    6Xhis tag

    Expression

    Mammalian

    Mutation

    C55G, N132S

    Promoter

    CMV

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pOIMars1CyN

    Plasmid

    #75221

    Purpose

    Mammalian cell surface expression of fluorogen activating protein Mars1Cy

    Depositor

    Alan Waggoner

    Article

    Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

    Insert

    ss-HA-Mars1Cy-CD80(237-306)
    

    Tags

    HA epitope

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTK-EdnrbE2-Citrine

    Plasmid

    #127770

    Purpose

    Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Citrine activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    ednrbE2
    

    Use

    Enhancer reporter construct

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PIGAZFN-R2

    Plasmid

    #37800

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    PIGA zinc finger R2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. THI VP64 pGAP T5

    Plasmid

    #161492

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T5 in the THI11 promoter)
    

    Expression

    Yeast

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. THI VP64 pGAP T1

    Plasmid

    #161488

    Purpose

    CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2

    Depositor

    Diethard Mattanovich, Brigitte Gasser

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Inserts

    dCAS9
    MS2-VP64
    gRNA (Targeting T1 in the THI11 promoter)
    

    Expression

    Yeast

    Promoter

    pGAP, pPOR1, and pTEF2

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLMB577

    Plasmid

    #83778

    Purpose

    Phenylalanine biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    promoter of RL1860
    

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKI-cjVASA-tdTomato

    Plasmid

    #160872

    Purpose

    Marmoset Targeting

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al Stem Cell Res. 2021 Mar 24;53:102308. do

    Insert

    tdTomato
    

    Use

    Marmoset targeting

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLI_C-tag TNF linker mCherry

    Plasmid

    #171181

    Purpose

    inducible expression of mCherry fused to C-tag TNF

    Depositor

    Veit Hornung

    Article

    Pinci et al J Biol Chem. 2020 Dec 25;295(52):18065-1

    Insert

    C-tag TNF linker mCherry
    

    Use

    Lentiviral

    Tags

    C-tag (internal) and mCherry

    Expression

    Mammalian

    Promoter

    tight TRE promoter

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLI_BFP_C-tag TNF

    Plasmid

    #171187

    Purpose

    inducible expression of C-tag TNF

    Depositor

    Veit Hornung

    Article

    Pinci et al J Biol Chem. 2020 Dec 25;295(52):18065-1

    Insert

    C-tag TNF
    

    Use

    Lentiviral

    Tags

    C-tag (internal)

    Expression

    Mammalian

    Mutation

    ADAM cleavage site of TNF (AA73-76) was exchanged…

    Promoter

    tight TRE promoter

    Available Since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. SH4-FRB-mRFP

    Plasmid

    #100741

    Purpose

    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    Fyn
    

    Tags

    FRB and mRFP

    Expression

    Mammalian

    Mutation

    SH4 domain only

    Promoter

    CMV

    Available Since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3 Glt1a

    Plasmid

    #98705

    Purpose

    mammalian expression of Glt1a

    Depositor

    Paul Rosenberg

    Article

    Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

    Insert

    Glt1a (Slc1a2 Rat)
    

    Expression

    Mammalian

    Mutation

    I288V (please see depositor comments below)

    Promoter

    CMV

    Available Since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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22. pILGFP3

    Plasmid

    #65451

    Purpose

    pILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Type

    Empty backbone

    Tags

    yEGFP reporter

    Expression

    Yeast

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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23. pITGFP3

    Plasmid

    #65452

    Purpose

    pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Type

    Empty backbone

    Tags

    yEGFP-CLN2PEST reporter

    Expression

    Yeast

    Available Since

    June 2, 2015

    Availability

    Academic Institutions and Nonprofits only

24. A3G-Cas9n-UGI

    Plasmid

    #119139

    Purpose

    Base editor made from APOBEC3G

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3G-Cas9 nickase-UGI
    

    Use

    CRISPR

    Mutation

    None

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. pMRX-IP/SECFP-mp62

    Plasmid

    #58785

    Purpose

    Expresses SECFP-mp62 in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    Sequestosome-1 (Sqstm1 Mouse)
    

    Use

    Retroviral

    Tags

    SECFP

    Available Since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

26. PIGAZFN-L2

    Plasmid

    #37798

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    PIGA zinc finger L2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAP1638

    Plasmid

    #137089

    Purpose

    Plasmid for PCR-generated donor DNA for rapid tagging of proteins with 4*(GFP11)

    Depositor

    Geraldine Seydoux

    Article

    Paix et al Curr Protoc Mol Biol. 2019 Dec;129(1):e1

    Insert

    4*(GFP11)
    

    Use

    Pcr template for donor dna

    Expression

    Bacterial

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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28. p alpha-actinin:GAL4/UAS:TdTomato

    Plasmid

    #161712

    Purpose

    Express TdTomato through amplification of GAL4/UAS system

    Depositor

    Hollis Cline

    Article

    Hiramoto et al Proc Natl Acad Sci U S A. 2014 Nov 25;11

    Insert

    TdTomato, GAL4
    

    Use

    Xenopus laevis expression

    Tags

    TdTomato

    Expression

    Mammalian

    Promoter

    actin-GAL4, UAS

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. PcDNA-DEST53-CypB-Golgi N-term GFP

    Plasmid

    #36127

    Depositor

    Patrick Van Oostveldt

    Article

    Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

    Insert

    cyclophilin B (PPIB Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains amino acids 1-211 of cyclophilin B

    Promoter

    CMV

    Available Since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pLMB686

    Plasmid

    #83780

    Purpose

    myo-Inositol biosensor plasmid

    Depositor

    Philip Poole

    Article

    Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

    Insert

    upstream region of RL4655 (intA)
    

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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